Conclusion
Lanthanides, as alternative probes to conventional fluorophores, can lead to enhanced sensitivity in applications where autofluorescence is a problem. Multiple labeling with lanthanide chelates have been shown to be an effective method for further increases in sensitivity. These probes, being nonisotopic, avoid the many practical problems associated with radioactive probes. Lanthanide probes are particularly advantageous in resonance energy transfer, whether for measuring distances in biocomplexes or when used to generate new lifetime tailored dyes.
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Selvin, P.R. (2003). Lanthanide-Labeled DNA. In: Lakowicz, J.R. (eds) Topics in Fluorescence Spectroscopy. Topics in Fluorescence Spectroscopy, vol 7. Springer, Boston, MA. https://doi.org/10.1007/0-306-47947-8_6
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