Abstract
The bilayer hydrophobic thickness l is estimated from the 2H-NMR spectra of acyl chain perdeuteriated membrane phospholipids. Values obtained from individual quadrupolar splittings and those from the first moment of a powder spectrum are in close agreement. In the fluid (L a) phase, the thermal variation of dimyristoylphosphatidylcholine (DMPC), dimyristoylphosphatidic acid (DMPA) at pH 4.2 and 8.2, and DMPC: cholesterol (7:3) bilayer thicknesses are compared; 30% cholesterol increases the thickness of dMPC by approximately 4–5 Å, whatever the temperature. DMPC and DMPA (whatever the pH) have the same hydrophobic thickness near their respective T c, the gel-to-fluid phase transition temperature, and display a very similar l decrease when increasing the temperature. However, at high temperatures (60°C) DMPC is thinner than DMPA by approximately 1 Å. Steric, electrostatic, and temperature constraints are therefore seen to modulate the biomembrane hydrophobic thickness , as easily estimated by 2H-NMR.
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© 1992 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG
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Léonard, A., Maillet, J.C., Dufourcq, J., Dufourc, E.J. (1992). Bilayer thickness from lipid-chain dynamics: Influece of cholesterol and electric charges. A 2H-NMR approach. In: Helm, C., Lösche, M., Möhwald, H. (eds) Trends in Colloid and Interface Science VI. Progress in Colloid & Polymer Science, vol 89. Steinkopff. https://doi.org/10.1007/BFb0116337
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DOI: https://doi.org/10.1007/BFb0116337
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