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Bioreactor for mammalian cell culture

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Vertebrate Cell Culture II and Enzyme Technology

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 39/1))

Abstract

Purpose of this article is to review the current status of bioreactor design for mammalian cell culture. Morphological and biochemical features of two major mammalian cell groups, anchoragedependent and independent cells are proposed as a basis for different behavior at their cultivation. Different bioreactor configurations are systematically discussed through enumerating elementary physical phenomena and through stressing their physiological significance. Special considerations are given to those areas which are inherent to mammalian cell bioreactor.

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Abbreviations

a:

global air-liquid interface

b:

stirrer blade thickness

c:

exponent in Eq. (33) solids concentration

¯c:

mean solids concentration

C:

constant in Eq. (21)

CD :

drag coefficient

d:

impeller diameter

dc :

cell size

dp :

particle size

D:

(characteristic) vessel dimension (diameter)

D02 :

oxygen diffusivity in medium

F1,2 :

forces at flow

g:

gravitational constant

h:

homogeneous zone height

H:

liquid height

HZH:

homogeneous zone height (h/H, dimensionless)

ICS:

cell-impeller collision severity

kL :

mass transfer (surface) coefficient

kL :

a volumetric mass transfer coefficient

¯n:

average fluid velocity at a level (Eq. 3)

nb :

number of impeller blades

N:

impeller speed

Nb :

bubble concentration

Ni :

cell-impeller collision frequency

Np :

power number

Nmax :

maximum cell packing density

Ncs :

impeller speed at complete solids suspension

Nhs :

impeller speed at homogeneous suspension

P:

power input

r:

annulus radius

R1 :

inner cylinder radius

R2 :

outer cylinder radius

Re:

impeller Reynolds number

Rep :

particle Reynolds number

Rb :

bubble radius

Shs :

surface Sherwood number

T:

torque

TCS:

turbulent collision severity

u:

settling particle (cell) velocity

um :

spatial mean velocity

umm :

particle-fluid relative velocity

u′:

fluctuating fluid velocity component (coordinate x)

u ′f :

root mean square value of fluctuating velocity component of turbulence

¯ubase :

mean liquid velocity at the bottom

v:

fluid velocity cell volume

v′:

fluctuating fluid velocity component (coordinate y)

vt :

bubble terminal velocity

vsg :

superficial gas velocity

V:

liquid volume

x:

streamline flow component

y:

radial flow component

α:

cone-and-plate viscometer angle

Β:

coefficient in Eq. (12)

γ:

shear rate

ε:

energy dissipation rate

εj :

local energy dissipation rate

η:

characteristic length of micro-scale eddy (Kolmogoroff micro-scale)

Μ:

fluid viscosity

Ν:

kinematic viscosity

ϱ:

fluid density

‡ϱ:

phase density difference

ϱc :

cell density

Τ:

shear stress

Τr :

Reynolds stress

Τt :

turbulent stress

Τv :

viscous stress

ϕ:

particle (cell) volume fraction

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  1. Washington University, St. Louis, USA

    Ales Prokop

  2. Invitron Corporation, St. Louis, USA

    Morris Z. Rosenberg

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Prokop, A., Rosenberg, M.Z. (1989). Bioreactor for mammalian cell culture. In: Vertebrate Cell Culture II and Enzyme Technology. Advances in Biochemical Engineering/Biotechnology, vol 39/1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0051951

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  • DOI: https://doi.org/10.1007/BFb0051951

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-51026-0

  • Online ISBN: 978-3-540-46133-3

  • eBook Packages: Springer Book Archive

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