Abstract
The molecular mechanisms of product storage and release in secretion remain unknown. Mucins stored in secretory granules form a highly condensed polymer network. An important condition for mucin condensation is the shielding of their polyionic charges, which depends on the presence of large amounts of Ca2+ inside the secretory granule. Experiments conducted on rabbit respiratory goblet cells, and in isolated giant mucin granules of the slug Ariolimax columbianus, indicate that upon exocytosis the mucin network undergoes dramatic swelling, expanding its volume as much as 250 to 1000 fold, respectively. Swelling of the granular matrix is governed by Donnan equilibrium, and follows a typical first-order kinetics. Depending upon the composition of the medium, the exocytosed mucin network can be recondensed. The observed volume changes are reversible and exhibit the characteristic discontinuity, as well as the temperature and pH dependence found in polymer gel phase transitions.
The evidence reviewed here is consistent with the idea that the condensed conformation of secretory products during storage in the cell, and their hydrated conformation upon release from the cell, reflect the corresponding condensed and decondensed phases of a polymer gel. Product release in exocytosis would result from a polymer gel phase transition that is probably triggered by a polycation Zā„+2/Na+ ion exchange via the secretory pore.
Preview
Unable to display preview. Download preview PDF.
3 References
Flory PJ (1964) Principles of polymer chemistry. Cornell University Press, Ithaca
Fernandez JM, Villalon M, Verdugo P (1991) Biophys J 59: 1022
Viney C, Huber AE, Verdugo P (1993) Macromolecules 26: 852
Boat TF, Cheng PW (1980) Fed Proc 39: 3067
Boat TF, Cheng PW, Iyer RN, Carlson DM, Polony I (1976) Arch Biochem Biophys 177: 95
Carlstedt I, Sheehan JK (1984) In: Nuget J, O'Connor M (eds) Mucus and mucosa (Ciba Foundation Symposium 109). Pitman, London, p 157
Kaliner MA, Borson DB, Nadel JA, Shelhamer JH, Patow CA, Marom Z (1988) In: Kaliner MA, Barnes PJ (eds) The airways. Neural control in health and disease. Marcel Dekker, New York, p 575
Kent PW (1978) In: Nuget J, O'Connor M (eds) Symposium on respiratory tract mucus (Ciba Foundation Symposium new series). Pitman, London, p 155
Slayter HS, Lamblin G, Le Treut A, Galabert C, Houdret N, et al. (1984) Eur J Biochem 142: 209
Havez R, Roussel P (1976) In: Weiss EB, Sega MS (eds) Bronchial asthma: Mechanisms and therapeutics. Little, Brown, Boston, p 409
Kaliner M, Marom Z, Patow C, Shelhamer J (1984) J Allergy Clin Immunol 73: 318
Shelhamer JH, Marom Z, Logun C, Kaliner M (1984) Exp Lung Res 7: 149
Gibbons RA, Mattner, PE (1966) Int J Fertil 11: 366
Roberts GP (1976) Arch Biochem Biophys 173: 528
Lee WI, Verdugo P, Blandau RJ (1977) Gynec Invest 8: 254
Verdugo P, Tam PY, Butler JC (1983) Biorheology 20: 223
Harding SE, Rowe AJ, Creeth JM (1983) Biochem J 209: 893
Sheehan JK, Oates K, Carlstedt I (1986) Biochem J 239: 147
Silberberg A (1987) Biorheology 24: 605
Katchalsky A, Lifson S, Eisenberg HJ (1951) J Polymer Sci 7: 571
Donnan FG (1924) Chem Rev 1: 73
Tam PY, Verdugo P (1981) Nature 292: 340
Verdugo P (1984) In: Nuget J, O'Connor M (eds) Mucus and mucosa (Ciba Foundation Symposium 109). Pitman, London, p 212
Frizzell, RA (1988) Am Rev Respir Dis 138: S3
Tanaka T, Fillmore DJ (1979) J Chem Phys 70: 1214
Verdugo P, Aitken ML, Langley L, Villalon M (1987) Biorheology 24: 625
Aitken ML, Verdugo P (1989) In: Chantler EN (ed) Mucus and related topics. Plenum, New York, p 73
Verdugo P, Deyrup-Olsen L, Aitken ML, Villalon M, Johnson D (1987) J Dent Res 66: 506
Izutsu K, Johnson D, Schubert M, Wang E, Ramsey B. et al. (1985) J Clin Invest 75: 1951
Roomans GM, von Euler AM, Muller RM, Gilljman H (1986) J Submicroc Cytol 18: 613
Sasaki S, Nakagaki I, Mori H, Imai Y (1983) Jap J Physiol 33: 69
Tanaka T (1981) Sci Am 244: 124
Verdugo P, Deyrup-Olsen I, Martin AW, Luchtel DL (1992) In: Karalis TK (ed) Mechanics of swelling. Springer, Berlin Heidelberg New York, p 67
Verdugo P (1991) Am Rev Respir Dis 144: S33
Verdugo P (1986) Biophys J 49: 231a
Verdugo P (1990) Annu Rev Physiol 52: 157
Monck JR, Fernandez JM (1992) J Cell Biol 119: 1395
Forstner JF, Forstner GG (1975) Biochim Biophys Acta 386: 283
Crowther RS, Marriot C (1983) J Pharm Phamacol 36: 21
Uvnas B, Aborg CH (1977) Acta Physiol Scand 100: 309
Nanavati C, Fernandez FM (1993) Science 259: 963
Basbaum CB, Berthold J, Finkbeiner WE (1990) Annu Rev Physiol 52: 97
Cohen DV, Morrissey JJ, Hamilton JW, Shofstall RE, et al. (1981) Biochemistry 20: 4135
Winkler H, Westhead E (1980) Neuroscience 5: 1803
Palade H (1975) Science 189: 347
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
Ā© 1993 Springer-Verlag
About this chapter
Cite this chapter
Verdugo, P. (1993). Polymer gel phase transition in condensation-decondensation of secretory products. In: DuŔek, K. (eds) Responsive Gels: Volume Transitions II. Advances in Polymer Science, vol 110. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0021131
Download citation
DOI: https://doi.org/10.1007/BFb0021131
Received:
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-56970-1
Online ISBN: 978-3-540-47836-2
eBook Packages: Springer Book Archive