Abstract
Digitonin treatment of chloroplasts is widely used to obtain PS I and PS II particles in concentrations higher than 1 per cent. The digitonin influence (below 1 per cent) on the proton uptake, FeCN photoreduction and on the behaviour of spin probes in chloroplast membranes is described in this study. Glutaraldehyde (GA) is a cross-linking reagent for soluble and membrane bound proteins and makes inter- and intramolecular bonds. GA treated chloroplasts preserve the partial ability for photoinduced transmembrane proton transport, O2 evolution, photoreduction of FeCN, lose the photophosphorylation activity and are osmotically inert. The influence of a GA on the molecular organization is not quite clear.
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© 1984 Springer Science+Business Media Dordrecht
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Kafalieva, D., Apostolova, E. (1984). Composition and Functions of Chloroplast and Thylakoid Membranes Affected by Digitonin and Glutaraldehyde. In: Sybesma, C. (eds) Advances in Photosynthesis Research. Advances in Agricultural Biotechnology, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-4973-2_8
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DOI: https://doi.org/10.1007/978-94-017-4973-2_8
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-247-2944-9
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