Abstract
Stacking of thylakoid membranes leads to the separation of Photosystem II and Photosystem I into appressed and non-appressed membrane regions. The associated increase in chlorophyll fluorescence is currently believed to reflect a decrease in spillover of exitation energy from Photosystem II to Photosystem I (Barber, 1980). Following kinetic analysis it has been suggested that the process can be interpreted in terms of a diffusion-limited movement of Photosystem II complexes (Rubin et al., 1981; Jennings et al., 1982). Lateral diffusion of large protein complexes in a biological membrane is highly dependent on physico-chemical parameters such as membrane fluidity. We have altered the thylakoid membrane composition artificially in order to change the membrane fluidity and observed the corresponding changes in the salt induced fluorescence rise. Thylakoids were rigidified by incorporation of cholesteryl hemisuccinate and the local anaesthetic benzyl alcohol was used as membrane fluidising agent.
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References
Barber J (1980) An explanation for the relationship between salt-induced thylakoid stacking and the chlorophyll fluorescence changes associated with changes in spillover of energy from Photosystem II to Photosystem I, FEBS Lett. 118, 1–10.
Ford RC, Chapman DJ, Barber J, Pedersen JZ and Cox RP (1982) Fluorescence polarization and spin-label studies of the fluidity of stromal and granal chloroplast membranes, Biochim. Biophys. Acta 681, 145–151.
Jennings RC, Gerola PD, Garlaschi FM and Forti G (1982) Studies on the kinetics of cation-associated fluorescence changes in chloroplast membranes, FEBS Lett. 142, 167–170.
Nakatani HY and Barber J (1977) An improved method for isolating chloroplasts retaining their outer membranes, Biochim. Biophys. Acta 461, 510–512.
Needham L, Whetton AD and Houslay MD (1982) The local anaesthetic and bilayer fluidising agent benzyl alcohol decreases the thermostability of the integral membrane protein adenylate cyclase, FEBS Lett. 140, 85–88.
Rubin BT, Barber J, Paillotin G, Chow WS and Yamamto Y (1981) A diffusional analysis of the temperature sensitivity of the Mg2+-induced rise of chlorophyll fluorescence from pea thylakoid membranes, Biochim. Biophys. Acta 638, 69–74.
Yamamoto Y, Ford RC and Barber J (1981) Relationship between thylakoid membrane fluidity and the functioning of pea chloroplasts, Plant Physiol. 67, 1069–1072.
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© 1984 Springer Science+Business Media Dordrecht
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Pedersen, J.Z., Cox, R.P. (1984). Relationship between Thylakoid Membrane Fluidity and the Kinetics of Salt Induced Fluorescence Changes: a Spin Label Study. In: Sybesma, C. (eds) Advances in Photosynthesis Research. Advances in Agricultural Biotechnology, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-4973-2_11
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DOI: https://doi.org/10.1007/978-94-017-4973-2_11
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-247-2944-9
Online ISBN: 978-94-017-4973-2
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