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Protoplast Technology in Grapevine

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Molecular Biology & Biotechnology of the Grapevine

Abstract

Hastein (1880) was probably the first to use the term protoplast, from the greek proto (πρώτο = first) and plastos (πλαστός = created), for plant cells lacking cell walls and to isolate protoplasts from cells of Vacuheria. In 1892, Klercker was the first to isolate protoplasts following plasmolysis of leaf tissue cells of Stratiotes aloides. Large scale isolation of protoplasts was started in the 60’s, when cell wall hydrolytic enzymes were employed (Cocking, 1961).

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Authors and Affiliations

  1. Department of Biology, University of Crete, P.O.Box 2208, 71409, Heraklion, Crete, Greece

    A. Papadakis & K. A. Roubelakis-Angelakis

  2. Centrum Grüne Gentechnik, SLFA Neustadt, Breitenweg 71, D67435, Neustadt/Weinstrasse, Germany

    G. Reustle

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  1. A. Papadakis
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  2. G. Reustle
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  3. K. A. Roubelakis-Angelakis
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Editors and Affiliations

  1. Department of Biology, University of Crete, P.O.Box 2208, 71409, Heraklion, Crete, Greece

    Kalliopi A. Roubelakis-Angelakis (Professor of Plant Physiology and Biotechnology, President) (Professor of Plant Physiology and Biotechnology, President)

  2. Federation of European Societies of Plant Physiology, Greece

    Kalliopi A. Roubelakis-Angelakis (Professor of Plant Physiology and Biotechnology, President) (Professor of Plant Physiology and Biotechnology, President)

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Papadakis, A., Reustle, G., Roubelakis-Angelakis, K.A. (2001). Protoplast Technology in Grapevine. In: Roubelakis-Angelakis, K.A. (eds) Molecular Biology & Biotechnology of the Grapevine. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2308-4_14

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  • DOI: https://doi.org/10.1007/978-94-017-2308-4_14

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