Abstract
Broccoli (Brassica oleracea var. italica) is a vegetable cole crop of considerable interest to breeders and seed companies since its area of production has increased in recent years mainly due to improved nutritional qualities, e.g. anticancer properties. Breeding companies strive more and more to bring F1 hybrid seeds onto the market. F1cultivars ensure high uniformity, offer high yields due to heterosis, allow rapid production and selection of desired genotypes and provide protection of plant breeder’s rights and the markets of seed companies. The production of hybrid cultivars requires homozygous parental lines. Inbred lines in broccoli can be produced by recurrent selfing, a procedure that takes time (6 to 7 generations of selfing) and is labour intensive due to sporophitic self-incompatibility. An alternative way to obtain pure inbred lines in one generation is the production of doubled haploid (DH) lines by microspore culture. This protocol can also be applied to other cole crops such as head and tronchuda cabbages using small adaptations.
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References
Dias, J.S., 1999. Effect of activated charcoal on Brassica oleracea microspore culture embryogenesis. Euphytica 108: 65–69.
Dias, J.S., 2001. Effect of incubation temperature regimes and culture medium on broccoli microspore culture embrogenesis. Euphytica 119: 389–394.
Duijs, J.G., R.E. Voorrips, D.L. Visser and J.B.M. Custers, 1992. Microspore culture is successful in most crop types of Brassica oleracea L. Euphytica 60: 45–55.
Gland, A., R. Lichter and H.G. Schweiger, 1988. Genetic and exogenous factors affecting embryogenesis in isolated microspore cultures of Brassica napus L. J.Plant Physiol. 132: 613617.
Lichter, R., 1982. Induction of haploid plants from isolated pollen of Brassica napus. Z.Pflanzenphysiol. 105: 427–434.
Nitsch, C. and J.P. Nitsch, 1967. The induction of flowering in vitro in stem segments of Plumbago indica L. I. The production of vegetative buds. Planta 72: 355–370.
Takahata, Y. and W.A. Keller, 1991. High frequency embryogenesis and plant regeneration in isolated microspore culture of Brassica oleracea L. Plant Sci. 74: 235–242.
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© 2003 Springer Science+Business Media New York
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da Silva Dias, J.C. (2003). Protocol for broccoli microspore culture. In: Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. (eds) Doubled Haploid Production in Crop Plants. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1293-4_30
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DOI: https://doi.org/10.1007/978-94-017-1293-4_30
Publisher Name: Springer, Dordrecht
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