Abstract
Pipette 1 ml of supernatant A (see Section 5) into the above mentioned culture tubes, remove acetone in a vacuum oven adjusted at 40°C (pressure 300 mbar). It takes about 3 h to remove acetone. Acetone can also be removed by flushing with nitrogen gas. Dry the contents completely in a heating block at 40°C by flushing with nitrogen. It takes about 25 min to dry the contents completely. It can be stored under nitrogen in a freezer.
(According to Makkar and Becker [8])
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© 2003 Springer Science+Business Media Dordrecht
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Makkar, H.P.S. (2003). Gallotannin Determination Using HPLC. In: Quantification of Tannins in Tree and Shrub Foliage. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0273-7_6
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DOI: https://doi.org/10.1007/978-94-017-0273-7_6
Publisher Name: Springer, Dordrecht
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