Abstract
One of the objectives in genome research is the integration of genetic, molecular and cytogenetic maps. A major step towards the realization of this challenging goal involves the localization of the physical positions of DNA sequences within an entire set of chromosomes by fluorescence in situ hybridization (FISH). This technique permits the identification of entire genomes, individual chromosomes, subchromosomal regions and single copy genes and is, therefore, a highly versatile tool in studying a variety of genomic issues, e.g. interspecific hybrids, chromosome rearrangements, distribution of repetitive DNA sequences and gene mapping.
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Fransz, P., de Jong, H., Zabel, P. (1998). Preparation of extended DNA fibres for high resolution mapping by fluorescence in situ hybridization (FISH). In: Gelvin, S.B., Schilperoort, R.A. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5242-6_4
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DOI: https://doi.org/10.1007/978-94-011-5242-6_4
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