Summary
Tobacco mosaic virus (TMV) was first studied scientifically as a plant pathogen 100 years ago. A form of hypersensitive resistance transferred from the wild species Nicotiana glutinosa to cultivated tobacco was shown 60 years ago to be due to a single dominant gene, named N. This causes the virus to be localised to necrotic lesions which form around each site of infection: the hypersensitive response (HR). N-gene resistance has proved extremely durable: only one virulent (resistance-breaking) TMV isolate has been reported to date. Another resistance gene, Nā, thought to be allelomorphic with N, causes a hypersensitive reaction to avirulent isolates of TMV, but numerous virulent isolates are also known. These do not induce necrosis but spread systemically and cause normal mosaic symptoms. The single known example of virulence against N has been mapped on TMV RNA to the replicase gene, whereas virulence against Nā in different TMV isolates has been mapped to a number of locations, all within the coat protein gene. The N gene has been isolated and sequenced: it shows structural and possibly functional features in common with certain other genes for resistance to bacterial and fungal pathogens, and to other genes with known functions in control of development or response to hormones in animals. These similarities give some clues about how the N-gene product might be involved in TMV recognition and in signalling the cascade of resistance and other responses which follows. The actual mechanism which inhibits TMV spread or multiplication after resistance is induced is not yet fully clear, but may involve an inhibition of multiplication or blocking of cell-to-cell spread of the infection front.
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Abbreviations
- HR:
-
hypersensitive response
- TMV:
-
tobacco mosaic virus
- PR:
-
pathogenesis-related protein
References
Bald JG (1960) Forms of tobacco mosaic virus. Nature 188: 645ā647
Barnett OW (1995) Plant virus disease ā economic aspects. In: Webster RG and Granoff A (eds) Encyclopedia of Virology, CD-ROM Edition. Academic Press, London
Beffa RS, Hofer RM, Thomas M and Meins F (1996) Decreased susceptibility to viral disease of Ī²-l,3-glucanase-deficient plants generated by antisense transformation. Plant Cell 8: 1001ā1011
Beijerinck MW (1898) Over een contagium vivum fluidum als oorzaak van de vlekziekte der tabaksbladen. Versl Gewone Vergad Afd Wis-Natuurkd Kon Akad Wetensch Amsterdam 7: 229ā235
Bent AF (1996) Plant disease resistance genes: function meets structure. Plant Cell 8: 1757ā1771
Bent AF, Kunkel BN, Dahlbeck D, Brown KL, Schmidt RL, Giraudat J, Leung JL and Staskawicz BJ (1994) RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes. Science 265: 1856ā1860
Brunt AA, Crabtree K, Dallwitz MJ, Gibbs AJ and Watson L (1996) Viruses of Plants. Descriptions and Lists from the VIDE Database. CAB International, Wallingford
Chivasa S, Murphy AM, Naylor M and Carr JP (1997) Salicylic acid interferes with tobacco mosaic virus replication via a novel salicylhydroxamic acid-sensitive mechanism. Plant Cell 9: 547ā557
Clausen RE and Goodspeed TH (1925) Interspecific hybridization in Nicotiana. II. A tetraploid glutinosatabacum hybrid, an experimental verification of Wingeās hypothesis. Genetics 10: 278ā284
Csillery G, Tobias I and Rusko J (1983) A new pepper strain of tomato mosaic virus. Acta Phytopathol Acad Sci Hung 18: 195ā200
Culver JN and Dawson WO (1989) Point mutations in the coat protein gene of tobacco mosaic virus induce hypersensitivity in Nicotiana sylvestris. Mol Plant-Microbe Interact 2: 209ā213
Da Graca JV and Martin MM (1976) An electron microscope study of hypersensitive tobacco infected with tobacco mosaic virus at 32Ā°C. Physiol Plant Pathol 8: 215ā219
Dawson WO, Bubrick P and Grantham GL (1988) Modification of the tobacco mosaic virus coat protein gene affecting replication, movement and symptomatology. Phytopathology 78: 783ā789
De Laat AMM and Van Loon LC (1983) Effects of temperature, light and leaf age on ethylene production and symptom expression in virus-infected tobacco leaves. Physiol Plant Pathol 22: 275ā283
Dinesh-Kumar SP, Whitham S, Choi D, Hehl R, Corr C and Baker B (1995) Transposon tagging of tobacco mosaic virus resistance gene N: its possible role in the TMV-N-mediated signal transduction pathway. Proc Natl Acad Sci USA 92: 4175ā4180
Dixon MS, Jones DA, Keddie JS, Thomas CM, Harrison K and Jones JDG (1996) The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins. Cell 84: 451ā459
Doke N and Ohashi Y (1988) Involvement of an O2 generating system in the induction of necrotic lesions on tobacco leaves infected with tobacco mosaic virus. Physiol Mol Plant Pathol 32: 163ā175
Dunigan DD, Golemboski DB and Zaitlin M (1987) Analysis of the N gene of Nicotiana. In: Evered D and Harnett S (eds) Plant Resistance to Viruses (Ciba Foundation Symposium 133), pp 120ā135.
John Wiley, Sons, Wiley Chichester Edelbaum O, Ilan N, Grafi G, Sher N, Stram Y, Novick D, Tal N, Sela I and Rubinstein M (1990) Two antiviral proteins from tobacco: purification and characterization by monoclonal antibodies to human interferon. Proc Natl Acad Sci USA 87: 588ā592
Edelbaum O, Sher N, Rubinstein M, Novick D, Tal N, Moyer M, Ward E, Ryals J and Sela I (1991) Two antiviral proteins, gp35 and gp22, correspond to Ī²-l,3-glucanase and an isoform of PR-5. Plant Mol Biol 17: 171ā173
Fraser RSS (1982) Are āpathogenesis-relatedā proteins involved in acquired systemic resistance of tobacco plants to tobacco mosaic virus? J Gen Virol 58: 305ā313
Fraser RSS (1985) Mechanisms involved in genetically controlled resistance and virulence: virus diseases. In: Fraser RSS (ed) Mechanisms of Resistance to Plant Diseases, pp 143ā196. Martinus Nijhoff/Dr W Junk, Dordrecht
Fraser RSS (1986) Genes for resistance to plant viruses. Crit Rev Plant Sci 3: 257ā294
Fraser RSS (1987) Biochemistry of Virus-Infected Plants. Research Studies Press, Letchworth/John Wiley and Sons, New York and Chichester
Gebhardt C (1997) Plant genes for pathogen resistance ā variation on a theme. Trends Plant Sci 2: 243ā244
Gera A, Loebenstein G, Saloman R and Frank A (1990) An inhibitor of virus replication (IVR) from protoplasts of a hypersensitive tobacco cultivar infected with tobacco mosaic virus, is associated with a 23K protein species. Phytopathology 80: 78ā81
Gera A, Tarn Y, Teverovsky E and Loebenstein G (1993) Enhanced tobacco mosaic virus production and suppressed synthesis of the inhibitor of virus replication in protoplasts and plants of local lesion responding cultivars exposed to 35Ā°C. Physiol Mol Plant Pathol 43: 299ā306
Gerstel DU (1948) Transfer of the mosaic-resistance factor between H chromosomes of Nicotiana glutinosa and N. tabacum. J Agric Res 76: 219ā223
Gierer A and Mundry KW (1958) Production of mutants of tobacco mosaic virus by chemical alteration of its ribonucleic acid in vitro. Nature 182: 1457ā1458
Goelet P, Lomonossoff GP, Butler PJG, Akam ME, Gait MJ and Kam J (1982) Nucleotide sequence of tobacco mosaic virus RNA. Proc Natl Acad Sci USA 79: 5818ā5822
Grant MR, Godiard L, Straube E, Ashfield T, Lewald J, Sattler A, Innes RW and Dangl JL (1995) Structure of the Arabidopsis RPM1 gene enabling dual specificity disease resistance. Science 269: 843ā846
Hammond-Kosack KE and Jones DGJ (1996) Resistance gene-dependent plant defence responses. Plant Cell 8: 1773ā1791
Holmes FO (1929) Local lesions in tobacco mosaic. Bot Gaz (Chicago) 87: 39ā55
Holmes FO (1938) Inheritance of resistance to tobacco mosaic virus in tobacco. Phytopathology 28: 553ā561
Holmes FO (1951) Indications of a New-World origin of tobacco mosaic virus. Phytopathology 41: 341ā349
Horvath J (1978) New artificial hosts and non-hosts of plant viruses and their role in the identification and separation of viruses. IV. Tobamovirus group: tobacco mosaic virus and tomato mosaic virus. Acta Phytopathol Acad Sci Hung 13: 57ā73
Ikeda R, Watanabe E, Watanabe Y and Okada Y (1993) Nucleotide sequence of tobamovirus Ob which can spread systemically in N gene tobacco. J Gen Virol 73: 1939ā1944
Jones DA and Jones JDG (1997) The roles of leucine-rich repeat proteins in plant defences. Adv Bot Res Adv Plant Pathol 24: 89ā167
Kleczkowski A (1950) Interpreting relationships between concentrations of plant viruses and numbers of local lesions. J Gen Microbiol 4: 53ā69
Klessig DF and Malamy J (1994) The salicylic acid signal in plants. Plant Mol Biol 26: 1439ā1458
Kobe B and Deisenhofer J (1995) A structural basis of the interactions between leucine-rich repeats and protein ligands. Nature 374: 183ā186
Konate G, Kopp M and Fritig B (1983) Studies on TMV multiplication in systemically and hypersensitively reacting tobacco varieties by means of radiochemical and immunoenzymatic methods. Agronomie 3: 95
Lartey L, Ghoshroy S, Sheng J and Citovsky V (1997) Transport through plasmodesmata and nuclear pores: cell-to-cell movement of plant viruses and nuclear import of Agrobacterium T-DNA. In: McCrae MA, Saunders JR, Smyth CJ and Stow ND (eds) Molecular Aspects of Host-Pathogen Interactions, pp 253ā280. Society for General Microbiology Symposium Series volume 55. Cambridge University Press, Cambridge
Lawrence GJ, Finnegan EJ, Ayliffe MA and Ellis JG (1995) The L6 gene for flax rust resistance is related to the Arabidopsis bacterial resistance gene RPS2 and the tobacco viral resistance gene N, Plant Cell 7: 1195ā1206
Leon J, Lawton MA and Raskin I (1995) Hydrogen peroxide stimulates salicylic acid biosynthesis in tobacco. Plant Physiol 108: 1673ā1678
Lewandowski DJ and Dawson WO (1995) Tobamoviruses. In: Webster RG and Granoff A (eds) Encyclopedia of Virology, CD-ROM Edition. Academic Press, London
Lucas WJ and Gilbertson RL (1994) Plasmodesmata in relation to viral movement within leaf tissues. Annu Rev Phytopathol 32: 387ā411
Matthews REF (1991) Plant Virology (Third Edition). Academic Press, San Diego.
Melchers G, Jockusch H and Sengbusch PV (1966) A tobacco mutant with a dominant allele for hypersensitivity against some TMV strains. Phytopathol Z 55: 86ā88
Meshi T, Ishikawa M, Motoyoshi F, Semba K and Okada Y (1986) In vitro transcription of infectious RNAs from full-length cDNAs of tobacco mosaic virus. Proc Natl Acad Sci USA 83: 5043ā5047
Mittler R, Shulaev V, Seskar M and Lam E (1996) Inhibition of programmed cell death in tobacco plants during a pathogen-induced hypersensitive response at low oxygen pressure. Plant Cell 8: 1991ā2001
Moser O, Gagey MJ, Godefroy-Colburn T, Stussi-Garaud C, Ellwart-Tschurtz M and Nitschko H (1988) The fate of the transport protein of tobacco mosaic virus in systemic and hypersensitive tobacco hosts. J Gen Virol 69: 1367ā1378
Osman TAM and Buck KW (1996) Complete replication in vitro of tobacco mosaic virus RNA by a template-dependent membrane-bound RNA polymerase. J Virol 70: 6227ā6234
Osman TAM and Buck KW (1997) The tobacco mosaic virus RNA polymerase complex contains a plant protein related to the RNA-binding subunit of yeast eIF-3. J Virol 71: 6057ā6082
Otsuki Y, Shimomura T and Takebe I (1972) Tobacco mosaic virus multiplication and expression of the N gene in necrotic responding tobacco varieties. Virology 50: 45ā50
Padgett HS and Beachy RN (1993) Analysis of a tobacco mosaic virus strain capable of overcoming N gene-mediated resistance. Plant Cell 5: 577ā586
Pfitzner UM and Pfitzner AJ (1992) Expression of a viral avirulence gene in transgenic plants is sufficient to induce the hypersensitive defense reaction. Mol Plant-Microbe Interact 6: 318ā321
Salmeron JM, Barker SJ, Carland FM, Mehta AY and Staskawicz BJ (1994) Tomato mutants altered in bacterial disease resistance provide evidence for a new locus controlling pathogen recognition. Plant Cell 6: 511ā520
SanfaƧon H, Cohen JV, Elder M, Rochon DM and French CJ (1993) Characterization of Solanum dulcamara yellow fleck-Ob, a tobamovirus that overcomes the N resistance gene. Phytopathology 83: 400ā404
Sela I, Grafi G, Sher N, Edelbaum O, Yagev H and Gerassi E (1987) Resistance systems related to the N gene and their comparison with interferon. In: Evered D and Harnett S (eds) Plant Resistance to Viruses (Ciba Foundation Symposium 133), pp 109ā119. John Wiley and Sons, Chichester
Sgro J-Y (1995) Special section on virus visualization. In: Webster RG and Granoff A (eds) Encyclopedia of Virology, CD-ROM version. Academic Press, London
Shaw JG, Plaskitt KA and Wilson TMA (1986) Evidence that tobacco mosaic virus particles disassemble co-translationally in vivo. Virology 148: 326ā336
Siegel A (1960) Studies on the induction of tobacco mosaic virus mutants with nitrous acid. Virology 11: 156ā167
Smart TE, Dunigan DD and Zaitlin M (1987) In vitro translation products of mRNAs derived from TMV-infected tobacco exhibiting a hypersensitive response. Virology 158: 461ā464
Takahashi T (1975) Studies on viral pathogenesis in plant hosts: VIII. Systemic virus invasion and localization of infection inā samsun-NNā plants resulting from tobacco mosaic virus infection. Phytopathol Z 84: 75ā87
Taraporewala ZF and Culver JN (1996) Identification of an elicitor active site within the three-dimensional structure of the tobacco mosaic virus tobamovirus coat protein. Plant Cell 6: 169ā178
Taraporewala ZF and Culver JN (1997) Structural and functional conservation of the tobamovirus coat protein elicitor active site. Mol Plant-Microbe Interact 10: 597ā604
Tenhaken R, Levine A, Brisson LF, Dixon RA and Lamb C (1995) Function of the oxidative burst in hypersensitive disease resistance. Proc Natl Acad Sci USA 92: 4158ā4163
Terry BR and Robards AW (1987) Hydrodynamic radius alone governs the mobility of molecules through plasmodesmata. Planta 171: 145ā157
Valleau WD (1952) The evolution of susceptibility to tobacco mosaic virus in Nicotiana and the origin of the tobacco mosaic virus. Phytopathology 42: 40ā42
Weber H, Haeckel P and Pfitzner AJP (1992) A cDNA clone of tobacco mosaic virus is infectious in plants. J Virol 66: 3909ā3912
Weber PVV (1951) Inheritance of a necrotic-lesion reaction to a mild strain of tobacco mosaic virus. Phytopathology 41: 593ā609
Whitham S, Dinesh-Kumar SP, Choi D, Hehl R, Corr C and Baker B (1994) The product of the tobacco mosaic virus resistance gene N: similarity to Toll and the interleukin-1 receptor. Cell 78: 1101ā1115
Whitham S, McCormick S and Baker B (1996) The N gene of tobacco confers resistance to tobacco mosaic virus in transgenic tomato. Proc Natl Acad Sci USA 93: 8776ā8781
Wilson TMA, Plaskitt KA, Watts JW, Osbourn JK and Watkins PAC (1990) Signals and structures involved in early interactions between plants and viruses or pseudoviruses. In: Fraser RSS (ed) Recognition and Response in Plant-Virus Interactions, pp 123ā145. Springer Verlag, Heidelberg
Wolf S, Deom CM, Beachy RN and Lucas WJ (1989) Movement protein of tobacco mosaic virus modifies plasmodesmatal size exclusion limit. Science 246: 377ā379
Wu X and Shaw JG (1997) Evidence that a viral replicase protein is involved in the disassembly of tobacco mosaic virus particles in vivo. Virology 239: 426ā434
Zhu Q, Maher EA, Masoud S, Dixon RA and Lamb CJ (1994) Enhanced protection against fungal attack by constitutive co-expression of chitinase and glucanase genes in transgenic tomato. Bio/Technology 12: 807ā812
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Fraser, R.S.S. (2000). Case Studies. In: Slusarenko, A.J., Fraser, R.S.S., van Loon, L.C. (eds) Mechanisms of Resistance to Plant Diseases. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3937-3_1
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