Abstract
Persistence on acidic soils makes flatpea (Lathyrus sylvestris L.) a potentially valuable crop species for many parts of the world. Use of this high yielding, perennial legume for forage, however, has been limited because of the large quantity of a neurotoxin, 2,4-diaminobutyric acid (DABA), in the plant. Cultivar development has been hindered by the narrow germplasm base and the lack of information about the breeding behavior of the plant and the applicability of mutagenesis treatments and tissue culture procedures for production of low-DABA variants. This study showed that pollen dehiscence occurs at early-bud stage when petal color is first evident. Mid- or late-stage flowers produced the highest levels of germinable pollen. Tripping of the stigma was required for pollination. Cross- pollination resulted in significantly higher seed set than selfing. Intercrossed progeny produced by honeybee pollination exhibited a lower mean concentration of foliar DABA than that of the accessions used as parents. Within the effective range of 10.0 to 17.5 kR for gamma irradiation of Lathco flatpea seeds, reduction of both seedling height and plant survival was a linear function of dose. A procedure was developed to regenerate flatpea plants from hypocotyl-derived callus cultures. Callus was initiated on a modified Schenk-Hildebrandt, low-ammonium medium containing 50 μM indole acetic acid (IAA) and 2.5 μM zeatin. After two subcultures on this medium, calli were transferred to morphogenesis medium containing 0.1 μM IAA and 1.0 μM zeatin. Although rhizogenesis was the primary morphogenetic response, infrequent and variable whole-plant regeneration was observed. Regenerated plants did not show substantial reduction in DABA concentration.
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© 1991 Springer Science+Business Media Dordrecht
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Foster, J.G., Coulombe, B.A., Van Scoyoc, S.W., Veilleux, R.E. (1991). Intercrossing methods, mutagenesis treatments, and tissue culture techniques for expanding the genetic diversity of flatpea (Lathyrus sylvestris L.). In: Wright, R.J., Baligar, V.C., Murrmann, R.P. (eds) Plant-Soil Interactions at Low pH. Developments in Plant and Soil Sciences, vol 45. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3438-5_117
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DOI: https://doi.org/10.1007/978-94-011-3438-5_117
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