Abstract
Calibration of the C2H2 reduction assay is necessary in order to obtain accurate measurements of N2 fixation. The ratio of C2H2 reduced: N2 fixed may be significantly higher than the theoretical ratio of 3:1 due to H2 evolution (Hudd et al. 1980; Saito et al. 1980). The aim of this study was to determine the relationships between N2 fixation, C2H2 reduction and H2 evolution of Trifolium subtevranewn, Medicago truncatula and Acacia dealbata.
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References
Hopmans P, Chalk PM and Douglas LA (1983) Plant Soil (in press)
Hopmans P, Douglas LA and Chalk PM (1982) Soil Biol. Biochem. 14, 495–500.
Hudd CA, Lloyd-Jones CP and Hill-Cottingham DG (1980) Physiol. Plant. 48, 111–115.
Saito SMT, Matsui E and Salati E (1980) Physiol. Plant. 49, 37–42.
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© 1984 Martinus Nijhoff/Dr W. Junk Publishers, The Hague and Pudoc, Wageningen
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Hopmans, P., Chalk, P.M., Douglas, L.A. (1984). Nitrogen Fixation, Acetylene Reduction and Hydrogen Evolution by Legumes Grown in Pots. In: Veeger, C., Newton, W.E. (eds) Advances in Nitrogen Fixation Research. Advances in Agricultural Biotechnology, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6923-0_8
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DOI: https://doi.org/10.1007/978-94-009-6923-0_8
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