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Measurements of sediment toxicity of autotrophic and heterotrophic picoplankton by epifluorescence microscopy

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Sediment/Water Interactions

Part of the book series: Developments in Hydrobiology ((DIHY,volume 50))

Abstract

Sediment toxicity from Toronto (Ontario) and Toledo (Ohio) harbours to autotrophic and heterotrophic picoplankton was evaluated simultaneously using epifluorescent microscopy. The approach is a simple, fast and effective combination of autofluorescence and fluorescence probes — 1-anilino-8-naphthalene sulfonic acid. The procedure is ideally suited for use with sediment slurries since it can differentiate fluorescent biotic material against a background of abiotic sediment particles and detritus.

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© 1989 Kluwer Academic Publishers

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Severn, S.R.T., Munawar, M., Mayfield, C.I. (1989). Measurements of sediment toxicity of autotrophic and heterotrophic picoplankton by epifluorescence microscopy. In: Sly, P.G., Hart, B.T. (eds) Sediment/Water Interactions. Developments in Hydrobiology, vol 50. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2376-8_50

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  • DOI: https://doi.org/10.1007/978-94-009-2376-8_50

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-9007-0

  • Online ISBN: 978-94-009-2376-8

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