Abstract
The objective of this work is the evaluation of ability of a series of biochemical markers to verify chromosome composition of chromosome segregant materials or secondary lines obtained by crossing triticale and wheat. From all sytems essayed, isozymes of Glutamate Oxalacetate Transaminase, Malate Dehydrogenase and endosperm prolamins studied by SDS-PAGE, were the most effective. GOT, A-PAGE and 2D-PAGE offer the possibility to detect 6D(6A) and other chromosome substitutions.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Kahler AL, Hallauer AR, Gardner CO. Allozyme polymorphisms within and among open-pollinated and adapted exotic populations of maize. Theor Appl Genet 1986; 72: 592–601.
Marchylo BA. Identification of Canadian and American wheat cultivars by SDS gradient PAGE analysis of gliadin and glutenin subunits. Can J Plant Sci 1987; 67: 945–952.
McIntyre CL.. Variation at isozyme loci in Triticeae. Pl Syst Evol 1988; 160: 123–142.
Bernardo A, Jouve N, Soler C. Discriminaci6n de variedades de cebada (Hordeum vulgare L.) mediante electroforesis. 2. Andlisis de isoenzimas Investigaciôn agraria. Prod Prot Veget 1990; 5 (1): 25–52.
Poulik HD. Starch gel eletrophoresis in a discontinuous system of buffers. Nature (London) 1957; 180: 47.
Hart GE. Glutamate oxaloacetate transaminase isozymes of Triticum: evidence for multiple systems of triplicat estructural genes in hexaploid wheat. In: “Isozymes: III Developmental Biology” ( Markert, C. Ed.) New York: Academic Press, 1975: 637–657.
Salinas J, Benito C. Chromosomal location of malate dehydrogenase structural genes in rye (Secale cereale L.). Z. Pflanzenzchtg 1985; 94: 208–217.
Payne PI, Corfield KG, Holt LM, Blackman JA. Correlations between the inheritance of certain high-molecular-weight subunits of glutenin and breadmaking quality in progenie of six crosses of bread wheat. J Sci Food Agric 1981; 32: 5160.
Lafiandra D, Kasarda D. One-and two-dimensional (two-pH) polyacrilamide gel electrophoresis in a single gel separation of wheat ptoteins. Cereal Chem 1985; 62: 314–319.
Tercero JA, Bernardo A, Jouve N. Encoding genes for endosperm proteins in Hordeum chilense. Theor Appl Genet 1990; 81: 127–132.
Rubio P, Jouve N, Soler C, Bernardo A. Genetic variation for isozymes and proteins in lines of the synthetic amphiploid x Triticosecale Wittmack. Genetics & Breeding 1994;(in press).
Tang KS, Hart GE. Use of isozymes as chromosome markers in wheatÄrye addition lines and in triticale. Genet Res Camb 1975; 26: 187–201.
Scandalios JG Genes, isozymes and evolution. Isozymes IV: Genetics and evolution. San Francisco: Academic press, Inc, 1975.
Hart GE, Langston Pi. Chromosome location and evolution of isozyme structural genes in hexaploid wheat. Heredity 1977; 39: 263–277.
Hart GE, Tuleen NA. Introduction and characterization of alien genetic material. Isozymes in Plant Genetics and Breeding, Part A. Amsterdam, Elsevier Science Publishers, 1983:: 339–362.
Diaz F, Jouve N. Structure of isozymes of the AAt-2 and AAT-3 systems of Aspartate Amino transaminase in Triticineae. Euphytica 91986; 35: 129–135.
Hart GE. Biochemical loci of hexaploid wheat (Triticum aestivum, 2n=42, genomes AABBDD). Genetics Maps 1984 Vol 3: 485–490.
Bergman CW, Salinas J. Isozyme variants of esterase and malate dehydrogenase among wheat aneuploids. Agron Abstr 1972; 23.
Benito C, Salinas J. The chromosomal location of malate dehydrogenase isozyme in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet 1983; 64: 255–258.
Diaz F, Fernandez JA, Jouve N. Structure and chromosomal location of malate dehydrogenase (Zone 2) isozymes in common and durum wheats. Euphytica 1986; 35: 509–513.
Sozinov A. 1984. Blocks of cereal storage proteins as genetic markers. Proc 2nd Int Workshop on Gluten Proteins, Wageningen: 121–127.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Kluwer Academic Publishers
About this chapter
Cite this chapter
Rubio, P., Soler, C., Bernardo, A., Jouve, N. (1996). Isozyme and Endosperm Protein Markers in the Determination of Chromosomal Constitution in X Triticosecale Wittmack. In: Guedes-Pinto, H., Darvey, N., Carnide, V.P. (eds) Triticale: Today and Tomorrow. Developments in Plant Breeding, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0329-6_54
Download citation
DOI: https://doi.org/10.1007/978-94-009-0329-6_54
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6634-1
Online ISBN: 978-94-009-0329-6
eBook Packages: Springer Book Archive