Abstract
Many attempts have been made to culture bee cells without success. However, several cell lines have been established from the sawfly (Sohi and Ennis, 1981) and from endoparasitoid wasps (Lynn and Hung, 1986, 1991). The culture media used for hymenopteran cell cultures are modified Grace’s medium and modified Goodwin’s IPL-52B (see Appendix 1).
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References
Giauffret, A., Quiot, J.M., Vago, C. and Poutiers, F. (1968) Cultures cellulaires d‘Apis mellifera. In: Barigozzi, C. (ed) Proceeding of the Second International Colloq uium on Invertebrate Tissue Culture, Villa Carlotta, 1967. Istituto Lombardo di Scienze e Lettre, Milano, pp 74–80.
Lynn, D.E. and Hung, A.C.F (1986) Development of a continuous cell line from the insect egg parasitoid, Trichogramma pretiosum (Hymenoptera: Trichogrammatidae). In Vitro Cell. Dev. Biol. 22, 440–442.
Lynn, D.E. and Hung, A.C.F. (1991) Development of continuous cell lines from the egg parasitoids Trichogramma confusum and T. exiguum. Arch. Insect Biochem. Physiol. 18, 99–104.
Sohi, S.S. and Ennis, T.J (1981) Chromosomal characterization of cell lines of Neodiprion lecontei (Hymenoptera: Diprionidae). Proc. Entomol. Soc. Ontario 112, 45–48.
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© 2002 Springer Japan
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Mitsuhashi, J. (2002). Insecta (Hymenoptera). In: Invertebrate Tissue Culture Methods. Springer Lab Manual. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67875-5_9
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DOI: https://doi.org/10.1007/978-4-431-67875-5_9
Publisher Name: Springer, Tokyo
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