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Insecta (Lepidoptera)

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Invertebrate Tissue Culture Methods

Part of the book series: Springer Lab Manual ((SLM))

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Abstract

Many continuous cell lines have been established from various tissues of lepidopteran insects, mostly for the purposes of study or the large-scale production of insect viruses.

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References

  • Carlson, J.G (1946) Protoplasmic viscosity changes in different regions of the grasshopper neuroblast during mitosis. Biol. Bull. 90, 109–121.

    Article  PubMed  CAS  Google Scholar 

  • Chao, J. and Ball, G.H (1971) A cell line isolated from hemocytes of Samia cynthia pupae. Curr. Top. Microbiol. Immunol. 55, 28–32.

    Article  PubMed  CAS  Google Scholar 

  • Eide, P.E., Coldwell, J.M. and Marks, E.P. (1975) Establishment of two cell lines from embryonic tissue of the tobacco hornworm, Manduca sexta (L.). In Vitro 11, 395–399.

    Article  PubMed  CAS  Google Scholar 

  • Grace, T.D.C. (1962) Establishment of four strains of cells from insect tissues grown in vitro. Nature 195, 788–789.

    Article  PubMed  CAS  Google Scholar 

  • Grace, T.D.C. (1967) Establishment of a line of cells from the silkworm, Bombyx mori. Nature 216, 613.

    Article  PubMed  CAS  Google Scholar 

  • Hara, K., Funakoshi, M., Tsuda, K. and Kawarabata, T. (1993) New Spodoptera exigua cell lines susceptible to Spodoptera exigua nuclear polyhedrosis virus. In Vitro Cell. Dev. Biol. 29A, 904–907.

    Article  CAS  Google Scholar 

  • Hink, W.F (1970) Established insect cell line from Trichoplusia ni. Nature 226, 466–467.

    Article  PubMed  CAS  Google Scholar 

  • Hink, W.F. and Ellis, B.J (1971) Establishment and characterization of two new cell lines (CP-1268 and CP-169) from the codling moth, Carpocapsa pomonella. Curr. Top. Microbiol. Immunol. 55, 19–28.

    Article  PubMed  CAS  Google Scholar 

  • Hink, W.F. and Ignoffo, CM (1970) Establishment of a new cell line (IMC-HZ-1) from ovaries of cotton ballworm moth, Heliothis zea (boddie). Exp. Cell Res. 60, 307–309.

    Article  PubMed  CAS  Google Scholar 

  • Inoue, H. and Mitsuhashi, J. (1984) A Bombyx mori cell line susceptible to a nuclear polyhedrosis virus. J. Sericul. Sci. Jpn. 53, 108–113.

    Google Scholar 

  • Loeb, M.J. and Hakim, R.S (1996) Insect midgut epithelium in vitro: an insect stem cell system. J. Insect Physiol. 42, 1103–1111.

    Article  CAS  Google Scholar 

  • Loeb, M.J., Jaffe, H., Gelman, D.B. and Hakim, R.S (1999) Two polypeptide factors that promote differentiation of insect midgut stem cells in vitro. Arch. Insect Biochem. Physiol. 40, 129–140.

    Article  PubMed  CAS  Google Scholar 

  • Lynn, D.E., Miller, S.G. and Oberlander, H. (1982) Establishment of a cell line from lepidopteran wing imaginal discs: induction of newly synthesized proteins by 20-hydroxyecdysone. Proc. Natl Acad. Sci. USA, Biol. Sci. 79, 2589–2593.

    Article  CAS  Google Scholar 

  • Lynn, D.E. and Oberlander, H. (1983) The establishment of cell lines from imaginal wing discs of Spodoptera frugiperda and Plodia interpunctella. J. Insect Physiol. 29, 591–596.

    Article  Google Scholar 

  • Lynn, D.E. and Oberlander, H. (1986) Obtainment of hormonally sensitive cell lines from imaginal discs of Lepidoptera species. In: Kurstak, E (ed.) Techniques in the Life Sciences, Vol. C-2, Techniques in in vitro invertebrate hormones and genes, C213, Elsevier Sci. Pub., County Clare, 1–12.

    Google Scholar 

  • Mano, C. and Mitsuhashi, J. (1981) Primary culture of various tissues in the greater wax moth, Galleria mellonella (Insecta, Lepidoptera). Zool. Mag. 90, 76–84 (in Japanese with an English abstract).

    Google Scholar 

  • Miltenburger, H.G., David, P., Mahr, U. and Zipp, W. (1977) Uber die Erstellung von Lepidopteren-Dauerzellinien und die in vitro-Replikation von insektenpathogenen Vieren. I. Mamestra brassicae L. (Kohleule)-Zellinien und NPV-Replikation. Z. F. Ang. Entomol. 82, 306–323.

    Google Scholar 

  • Mitsuhashi, J. (1965) Tissue culture of the rice stem borer, Chilo suppressalis Walker (Lepidoptera: Pyralidae). I. Cell migration from the explanted tissues of diapausing larvae. Jpn. J. Appl. Entomol. Zool. 9, 137–141.

    Google Scholar 

  • Mitsuhashi, J. (1972) Primary culture of hemocytopoietic tissue of Papilio xuthus Linné (Lepidoptera: Papilionidae). Appl. Entomol. Zool. 7, 39–41.

    Google Scholar 

  • Mitsuhashi, J. (1973) Establishment of cell lines from the pupal ovaries of the swallow tail, Papilio xuthus Linné (Lepidoptera: Papilionidae). Appl. Entomol. Zool. 8, 64–72.

    Google Scholar 

  • Mitsuhashi, J. (1977) Establishment and characterization of continuous cell lines from pupal ovaries of the cabbage armyworm, Mamestra brassicae (Lepidoptera, Noctuidae). Develop. Growth Differ. 19, 337–344.

    Article  CAS  Google Scholar 

  • Mitsuhashi, J. (1981) Establishment and some characteristics of a continuous cell line derived from fat bodies of the cabbage armyworm (Lepidoptera, Noctuidae). Develop. Growth Differ. 23, 63–72.

    Article  Google Scholar 

  • Mitsuhashi, J. (1983) A continuous cell line derived from fat bodies of the common armyworm, Leucania separata (Lepidoptera: Noctuidae). Appl. Entomol. Zool. 18, 533–539.

    Google Scholar 

  • Mitsuhashi, J. (1984) Isolation of continuous cell line from larval fat bodies of an arctiid moth, Spilarctia seriatopuntata (Insecta, Lepidoptera, Arctiidae). Zool. Sci. 1, 415–419.

    Google Scholar 

  • Mitsuhashi, J. and Shozawa, A. (1985) Continuous cell lines from larval hemocytes of the cabbage armyworm, Mamestra brassicae. Develop. Growth Differ. 27, 599–606.

    Article  Google Scholar 

  • Pant, U., Mascarenhas, A.F. and Jagannathan, V. (1977) In vitro cultivation of a cell line from embryonic tissue of potato tuber moth, Gnorimoschema operculella (Zeller). Indian J. Exp. Biol. 15, 244–245.

    Google Scholar 

  • Pant, U., Athawale, S.S., Sudeep, A.B. and Banerjee, K. (1997) A new cell line from larval ovaries of Spodoptera litura (Lepidoptera, Noctuidae). In Vitro Cell. Dev. Biol. 33A, 161–163.

    Article  Google Scholar 

  • Rochford, R., Dougherty, E.M. and Lynn, D.E (1984) Establishment of a cell line from embryos of the cabbage looper, Trichoplusia ni (Hübner). In vitro 20, 823–825.

    Article  Google Scholar 

  • Sadrud-Din, S.Y., Hakim, R.S. and Loeb, M.J (1994) Proliferation and differentiation of midgut epithelial cells from Manduca sexta, in vitro. Invert. Reprod. Dev. 26, 197–204.

    Article  Google Scholar 

  • Sadrud-Din, S.Y., Loeb, M.J. and Hakim, R.S. (1996) In vitro differentiation of isolated stem cells from the midgut of Manduca sexta larvae. J. Exp. Biol. 199, 319–325.

    Article  PubMed  Google Scholar 

  • Sato, T. (1989) Establishment of eight cell lines from neonate larvae of tortricids (Lepidoptera) and their several characteristics including susceptibility to insect viruses. In: Mitsuhashi, J. (ed) Invertebrate Cell System Applications, Vol. II. CRC, Boca Raton, pp 188–198.

    Google Scholar 

  • Sieburth, P.J. and Maruniak, J.E. (1988) Growth characteristics of a continuous cell line from the velvetbean caterpillar, Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae). In Vitro Cell. Dev. Biol. 24, 195–198.

    Article  Google Scholar 

  • Sohi, S.S. (1973) In vitro cultivation of larval tissue of Choristoneura fumiferana (Clemens) (Lepidoptera: Tortoricidae). In: Řeháček J., Blaškovié, D. and Hink, W.F. (eds) Proceedings of the 3rd International Colloquium. Invertebrate Tissue Culture, Bratislava, 1971. Publ. House of the Slovak Acad. Sci., Bratislava, 1971, pp 75–92.

    Google Scholar 

  • Stanley, M.S.M. and Vaughn, J.L (1968) Origin of migrating cells in cultures of moth ovarian tissue based on developmental stages producing optimum out growth. Ann. Entomol. Soc. Am. 61, 1067–1072.

    Google Scholar 

  • Takami, T. and Kitazawa, T. (1960) Tables of silkworm embryonic developmental Stages, Tech. Bull. Sericultural Exp. Sta. No.75, 1–31. (in Japanese)

    Google Scholar 

  • Trager, W. (1935) Cultivation of the virus of grasserie in silkworm tissue cultures. J. Exp. Med. 61, 501–513.

    Article  PubMed  CAS  Google Scholar 

  • Vaughn, J.L., Goodwin, R.H., Tompkins, G.J. and McCawley, P. (1977) Establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera, Noctuidae). In Vitro 13, 213–217.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan

    Jun Mitsuhashi (Professor)

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  1. Jun Mitsuhashi
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© 2002 Springer Japan

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Mitsuhashi, J. (2002). Insecta (Lepidoptera). In: Invertebrate Tissue Culture Methods. Springer Lab Manual. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67875-5_5

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  • DOI: https://doi.org/10.1007/978-4-431-67875-5_5

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-70313-6

  • Online ISBN: 978-4-431-67875-5

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