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Abstract

Attempts have been made to use cultured insect cells for the production of foreign proteins by stably transforming the cultured cells. For this purpose, usually the target gene and a gene that facilitates the selection of transformed cells such as antibiotics resistant gene are introduced at the same time. There are various methods to transfect cultured cells.

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References

  • Bourouis, M. and Jarry, B. (1983) Vectors containing a prokaryotic dihydrofolate reductase gene transform Drosophila cells to methotrexate-resistance. EMBO J. 2, 1099–1104.

    PubMed  CAS  Google Scholar 

  • Cherbas, L., Moss, R. and Cherbas, R. (1994) Transformation techniques for Drosophila cell lines. In: Goldstein, L. and Fyrberg, E. (eds) Methods in Cell Biology, Vol. 44. Academic, New York, pp 161–179.

    Google Scholar 

  • Di Nocera, P.P. and Dawid, I.B (1983) Transient expression of genes introduced into cultured cells of Drosophila. Proc. Natl Acad. Sci. USA 80, 7095–7098.

    Article  PubMed  Google Scholar 

  • Durbin, J.E. and Fallon, A.M (1985) Transient expression of the chloramphenicol acetyltransferase gene in cultured mosquito cells. Gene 36, 173–178.

    Article  PubMed  CAS  Google Scholar 

  • Echalier, G. (1997) Drosophila Cells in Culture, Academic Press, SanDiego, pp 702.

    Google Scholar 

  • Echalier, G. and Fourcade-Pernnet, F. (1994) Differences in transfection efficiency between Drosophila cell lines. In: Maramorosch, K. and Mcintosh, A.H. (eds) Insect Cell Biotechnology. CRC, Boca Raton, pp 141–156.

    Google Scholar 

  • Fallon, A.M (1986) Factors affecting polybrene-mediated transfection of cultured Aedes albopictus (Mosquito) cells. Exp. Cell Res. 166, 535–542.

    Article  PubMed  CAS  Google Scholar 

  • Graham, F.L. and Van der Eb, A.J (1973) A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52, 456–467.

    Article  PubMed  CAS  Google Scholar 

  • Jarvis, D.L., Fleming, J.G.W., Kovacs, G.R., Summers, M.D. and Guarino, L.A (1990) Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells. Biotechnology 8, 950–955.

    Article  PubMed  CAS  Google Scholar 

  • Kawai, S. and Nishizawa, M. (1984) New procedure for DNA transfection with polycation and dimethylsulfoxide. Mol. Cell. Biol. 4, 1172–1174.

    PubMed  CAS  Google Scholar 

  • McLachlin, J.R. and Miller, L.K (1997) Stable transformation of insect cells to coexpress a rapidly selectable marker gene and an inhibitor of apoptosis. In Vitro Cell. Dev. Biol. 33A, 575–579.

    Article  Google Scholar 

  • Maisonhaute, C. and Echalier, G. (1986) Stable transformation of Drosophila Kc cells to antibiotic resistance with the bacterial neomycin resistance gene. FEBS Lett. 197, 45–49.

    Article  PubMed  CAS  Google Scholar 

  • Malone, R.W., Feigner, P.L., and Verma, I.M. (1989) Cationic liposome-mediated RNA transfection. Proc. Natl Acad. Sci. USA 86, 6077–6081.

    CAS  Google Scholar 

  • Sinclair, J.H., Sang, J.H., Burke, J.F and Ish-Horowicz, D. (1983) Extra-chromosomal replication of copia-based vectors in cultured Drosophila cells. Nature 306, 198–200.

    Article  CAS  Google Scholar 

  • Van der Straten, A., Johansen, H., Sweet, R. and Rosenberg, M. (1988) Efficient expression of foreign genes in cultured Drosophila melanogaster cells using hygromycin B selection. In: Kuroda, Y., Kurstak, E. and Maramorosch, K. (eds) Invertebrate and Fish Tissue Culture. Springer, New York, 131–134.

    Chapter  Google Scholar 

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© 2002 Springer Japan

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Mitsuhashi, J. (2002). Gene Technology. In: Invertebrate Tissue Culture Methods. Springer Lab Manual. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67875-5_41

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  • DOI: https://doi.org/10.1007/978-4-431-67875-5_41

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-70313-6

  • Online ISBN: 978-4-431-67875-5

  • eBook Packages: Springer Book Archive

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