Abstract
Oocytes cultures have been made using the medium of Heacox et al., modified Cecil’s medium, or modified TC-199 medium (see Appendix 1). The following is the method of Heacox et al. (1983) to cultivate oocytes of the polychaete Nereis virens.
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References
Battaglia, M. and Davoli, C. (1997) Long-term tissue culture of the earthworm Eisenia foetida (Annelida, Oligochaeta). In: Maramorosch, K. and Mitsuhashi, J. (eds) Invertebrate Cell Culture: Novel Directions and Biotechnology Applications. Science Publishers, Enfield, pp 261–268.
Dietzel, I.D., Drapeau, P. and Nicholls J.G (1986) Voltage dependence of 5-hydroxytryptamine release at a synapse between identified leech neurons in culture. J. Physiol. 372, 191–205.
Fretter, V. and Graham, A. (1976) A Functional Anatomy of Invertebrates. Academic Press, London, pp.589.
Heacox, A.E., Fischer, A. and Frangenberg, H.R (1983) Development of a medium for in vitro culture of oocyte from the polychaete, Nereis virens. In Vitro 19, 825–832.
Krontowsky, A. and Rumianzew, A. (1922) Zur Technik der Gewebs-kulturen von Regenwürmern in vitro. Pflügers Arch. Gesamte Physiol. Menschen Tiere 195, 291–299.
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© 2002 Springer Japan
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Mitsuhashi, J. (2002). Annelida. In: Invertebrate Tissue Culture Methods. Springer Lab Manual. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67875-5_16
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DOI: https://doi.org/10.1007/978-4-431-67875-5_16
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