Abstract
Adenosine is a potent inhibitory modulator in the brain. It suppresses glutamatergic synaptic transmission and possibly acts as an endogenous brain neuroprotective agent. In this study we have examined the effects of a clinically used porcine brain tissue hydrolysate, Cerebrolysin®, on synaptic transmission in the CA1 area of the rat hippocampus. Hippocampal slices were prepared for in vitro experiments using standard procedures and perfused with artificial cerebrospinal fluid at 32°C and pH = 7.4. Field potentials were evoked by stimulating the Schaffer collateral/commissural afferents and recorded in the stratum radiatum of CA1 field. Cerebrolysin® or other pharmacological agents were dissolved in the perfusate prior to applications. The application periods were usually 5–15 minutes, sufficiently long to obtain steady state effects on synaptic transmission. A major effect of Cerebrolysin® at 10 μl/ml was a depression of synaptic transmission at the Schaffer collateral/ commissural pathway. Detailed analysis showed that the inhibition is presynaptic and can be reduced by a specific blocker of Al adenosine receptors, 8-cyclopentyltheophylline (100nM). Since Cerebrolysin does not contain a detectable amount of adenosine, the effect on adenosine receptors must be indirect, perhaps by release of an endogenous agonist. This action of Cerebrolysin® is consistent with the putative neuroprotective action underlying its clinical usage. Supported by EBEWE Initiative.
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© 1996 Springer-Verlag
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Wojtowicz, J.M., Xiong, H., Baskys, A. (1996). Brain tissue hydrolysate, Cerebrolysin®, acts on presynaptic adenosine receptors in the rat hippocampus. In: Jellinger, K.A., Windisch, M. (eds) New Trends in the Diagnosis and Therapy of Non-Alzheimer’s Dementia. Journal of Neural Transmission Supplement, vol 47. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6892-9_26
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DOI: https://doi.org/10.1007/978-3-7091-6892-9_26
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-82823-6
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