Abstract
Cells are able to respond in an extremely sensitive way to changes in their environment. Electronic microstructures on sensor chips can be applied to analyse such responses by recording properties of cell metabolism and cell morphology. These parameters are linked intimately to the cellular signalling network and can thus be used as sensitive indicators for any perturbation of cellular physiology like toxic insults or receptor activation events. Sensor chips might be able to fill a gap by providing functional and dynamic assays related to cell metabolism and cell morphology. Arraying of sensor chips is a prerequisite in order to make such novel types of cell-based assays work efficiently. It depends on technological efforts in chip packaging and electrical connections, electronic sensor control and specific modes of automated liquid handling. A presentation of solutions developed in the authors’ laboratory is given but other approaches in the field of sensor-based cellular analysis will also be included.
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Abbreviations
- MOSFET:
-
Metal oxide semiconducting field-effect transistor
- ISFET:
-
Ion-sensitive field-effect transistor
- IDES:
-
Interdigitated electrode structure
- LAPS:
-
Light-activated potentiometric sensor
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Brischwein, M., Grothe, H., Otto, A.M., Stepper, C., Weyh, T., Wolf, B. (2004). Living Cells on Chip: Bioanalytical Applications. In: Mirsky, V.M. (eds) Ultrathin Electrochemical Chemo- and Biosensors. Springer Series on Chemical Sensors and Biosensors, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-05204-4_7
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DOI: https://doi.org/10.1007/978-3-662-05204-4_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-05961-2
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