Abstract
The determination of molecular weight (MW) averages and molecular weight distributions (MWD) of water-soluble polymers, such as nonionic and ionic synthetic polymers, proteins and peptides, by aqueous SEC sometimes encounters difficulties because of non-size exclusion effects. SEC is a separation technique based on molecular size and any polymers eluted at the same retention volume should have the same molecular size making it possible to calculate the MW or MWD of a polymer using a calibration curve constructed with polymer standards that are different from the polymer under investigation. Moreover, it can be assumed that species that elute earlier from SEC columns have higher MWs than those of the same type of polymer eluted later.
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© 1999 Springer-Verlag Berlin Heidelberg
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Mori, S., Barth, H.G. (1999). Aqueous Size Exclusion Chromatography. In: Size Exclusion Chromatography. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03910-6_11
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DOI: https://doi.org/10.1007/978-3-662-03910-6_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-08493-5
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