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Site-Directed Mutagenesis of Wheat 9 kDa Lipid Transfer Protein (LTP)

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Plant Proteins from European Crops

Summary

Wheat 9 kDa LTP and four mutants were produced in Escherichia coli and compared with wheat-extracted LTP. Only the Y79A mutant was affected in lipid-binding activity, confirming the position of this residue in the three-dimensional structure of the protein.

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References

  • ARONDEL V., KADER J.C., 1990. Lipid transfer in plants. Experientia 46, 579–585.

    Article  PubMed  CAS  Google Scholar 

  • DIERYCK W., GAUTIER M-R., LULLIEN V., JOUDRIER P., 1992. Nucleotide sequence of a cDNA encoding a lipid transfer protein from wheat (Triticum durum Desf.). Plant Mol. Biol 19, 707–709.

    Article  PubMed  CAS  Google Scholar 

  • GINCEL E., SIMORRE J-P., CAILLE A., MARION D., PTAK M., VOVELLE F., 1994. Three-dimensional structure in solution of a wheat lipid-transfer protein from multidimensional 1H-NMR data. Eur. J. Biochem. 226, 413–422.

    Article  PubMed  CAS  Google Scholar 

  • GOLOUBINOFF P., GATENBY A.A., LORIMER G.H., 1989. Gro E heat-shock proteins promote assembly of foreign prokaryotic ribulose biphosphate carboxylase oligomers in E. coli. Nature 337, 44–47.

    Article  PubMed  CAS  Google Scholar 

  • GOMAR J., PETIT M-C., SODANO P., SY D., MARION D., KADER J.C., VOVELLE F., PTAK M., 1996. Solution structure and lipid binding of a non-specific lipid transfer protein extracted from maize seeds. Protein Science 5, 565–577.

    Article  PubMed  CAS  Google Scholar 

  • KUNKEL T.A., ROBERTS J.D., ZAKOUR R.A., 1987. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154, 367–382.

    Article  PubMed  CAS  Google Scholar 

  • BMOLINA A., GARCIA-OLMEDO F., 1993. Developmental and pathogen-induced expression of three barley genes encoding lipid transfer proteins. Plant J. 4, 983–991.

    Article  Google Scholar 

  • MONNET F-P., 1990. Caractérisation d’une protéine de fixation de lipides du blé dur, purification, séquençage, ADN complémentaire; relations aux protéines végétales de transfert de lipides et aux inhibiteurs d’amylases/trypsine des céréales. Thesis: Université des Sciences et Techniques du Languedoc, Montpellier, France, p. 1–121.

    Google Scholar 

  • ROSENBERG A.H., LADE B.N., CHUI D-S, LIN S-W., DUNN J.J., STUDIER F.W., 1987. Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene 56, 125–135.

    Article  PubMed  CAS  Google Scholar 

  • SANGER F., NICKLEN S, COULSON A.R., 1977. DNA sequencing with chain terminating inhibitors. Proc. Natl Acad. Set USA 74, 5463–5467.

    Article  CAS  Google Scholar 

  • SORENSEN S.B., BECH L.M., MULDBJERG M., BEENFELDT T., BREDDAM K., 1993. Barley lipid transfer protein 1 is involved in beer foam formation. MBAA T. Q. 30, 136–145.

    CAS  Google Scholar 

  • STERCK P., BOOIJ H., SCHELLEKENS G.A., van KAMMEN A., DE VRIES S.C., 1991. Cell-specific expression of the carrot EP2 lipid transfer protein gene. Plant Cell 3, 907–921.

    Google Scholar 

  • STUDIER F.W., ROSENBERG A.H., DUNN J.J., DUBENDORFF J.W., 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185, 60–89.

    Article  PubMed  CAS  Google Scholar 

  • TERRAS F.R.G., GODERIS I.J., VAN LEUNEN F., VANDERLEYDEN J., CAMMUE B.P.A., Broekaert W.F., 1992. In vitro antifungal activity of a radish (Raphanus sativus L.) seed protein homologous to nonspecific lipid transfer proteins. Plant Physiol. 100, 1055–1058.

    Article  PubMed  CAS  Google Scholar 

  • van PARIDON P.A., GADELLA T.W.J., SOMERHARJU P.J., WIRTZ K.W.A., 1987. On the relationship between the dual specificity of the bovine brain phosphatidylinositol transfer protein and membrane phosphatidylinositol levels. Biochim. Biophys. Acta 903, 68–77.

    Article  PubMed  Google Scholar 

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© 1998 Springer-Verlag Berlin Heidelberg

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Lullien-Pellerin, V. et al. (1998). Site-Directed Mutagenesis of Wheat 9 kDa Lipid Transfer Protein (LTP). In: Guéguen, J., Popineau, Y. (eds) Plant Proteins from European Crops. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03720-1_15

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  • DOI: https://doi.org/10.1007/978-3-662-03720-1_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-03722-5

  • Online ISBN: 978-3-662-03720-1

  • eBook Packages: Springer Book Archive

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