Abstract
Among the most refractory remaining physiological problems concerning sexual reproduction is the mechanism of interaction between the male and female gametes during fertilization. In Angiosperms, the double fertilization process is dependant on numerous events beginning with the deposition of the pollen grain on the stigmatic surface and ending with the double gametic fusion within the embryo sac. During the last decade a renewed interest in this subject has been triggered as a result of the discovery, through cytological studies, of cytoplasmic dimorphism of sperm cells (Russell 1983). In Plumbago, Russell (1985) has shown the occurence of a targeted fertilization process: One of the two sperm cells which is plastid-rich preferentially fuses with the egg cell and subsequently forms the embryo, the second (mitochondria rich) associates with the central cell to initiate endosperm formation.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
Literature References
Chaboud A., Perez R., Digonnet C., Dumas C. (1992) Gamete recognition in Angiosperms: model and strategy analysis.Society for experimental biology. Seminar series 48. Perspectives in plant recognition. J.A.Callow and J.R. Green (eds). In press.
Dupuis I., Roeckel P., Matthys-Rochon E., Dumas C. (1987). Procedure toisolate viable sperm cells from corn (Zea mays L.) pollen grains. Plant Physiol., 85: 876–878
Faure J.E., Mogensen H. L., Kranz E., Digonnet C., Dumas C. (1992) Ultrastructural characterization and three-dimensional reconstruction of isolated maize (Zea mays L.) egg cells. In preparation.
Heslop Harrison J. and Heslop Harrison Y. (1970) Evaluation of pollen viability by enzymatically induced fluorescence, intra cellular hydrolysis of fluoresceine diacetate, Stain Technol. 45: 115–120
Keijzer C.J., Reinders M.C., Leferink-ten Klooster H.B. (1988) A micromanipulation method for artificial fertilization in Torenia. In: Cresti M., Gori P., Pacini E.(eds) Sexual reproduction in higher plants. Springer, Berlin Heidelberg New york, pp 119–124
Kranz E., Bautor J., Lörz H. (1991a) In vitro fertilization of single, isolated gametes of maize mediated by electrofusion. Sex. Plant Reprod. 4: 12–18
Kranz E., Bautor J., Lörz H. (1991b) Electrofusion mediated transmission of cytoplasmic organelles through the in vitro fertilization process, fusion of sperm cells with synergids and central cells, and cell reconstitution in maize. Sex. Plant Reprod. 4: 17–21
Kranz E., Lörz H., Digonnet C., Faure J.E. (1992) In vitro fusion of gametes and production of zygotes. Int. Rev. Cyt. In press.
Matthys-Rochon, E., Vergne, P., Detchepare, S., Dumas C. (1987) Male germ unit isolation from three tricellular pollen species: Brassica oleracea, Zea mays and Triticum aestivum. Plant Physiol. 83: 464–466
Matthys-Rochon E. and C. Dumas (1988) The male germ unit: retrospect and prospects in: H.J.Wilms and C.J. Keijzer (eds) Plant sperm cells as tools for biotechnology Pudoc Wageningen, the Netherlands. pp 51–60
Matthys-Rochon E., Digonnet C., Dumas C. (1991) Microinjection of lucifer yellow into embryo sac cells of Zea mays. International symposium on Angiosperm pollen and ovules. Basic and applied aspects. Cômo-Italy
Mogensen H.L. (1988) Exclusion of male mitochondria and plastids during syngamy as a basis for maternal inheritance Proc. Natl. Acad. Sci. USA 85: 2594–2597
Russell S.D. (1983) Fertilization in Plumbago zeylanica: gametic fusion and fate of the male cytoplasm. Amer. J. Bot. 70: 416–434
Russell S.D. (1985) Preferential fertilization in Plumbago: ultra structural evidence for gamete level recognition in an Angiosperm. Proc. Natl. Acad. Sci. USA 82: 6129–6132
Theunis C.H., Pierson E.S., Cresti M. (1991) Isolation of male and female gametes in higher plants. Sex. Plant Reprod. 4: 145154
Wagner V.T., Dumas C., Mogensen H.L. (1989a) Morphometric analysis of isolated Zea mays sperm. Journal of Cell Science. 93: 179–184
Wagner V.T., Song Y.C., Matthys-Rochon E., Dumas C. (1989b) Observations on the isolated embryo sac of Zea mays. Plant Science. 59: 127–132
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Matthys-Rochon, E. (1992). In Vitro Fertilization in Flowering Plants. In: Dattée, Y., Dumas, C., Gallais, A. (eds) Reproductive Biology and Plant Breeding. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76998-6_19
Download citation
DOI: https://doi.org/10.1007/978-3-642-76998-6_19
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-77000-5
Online ISBN: 978-3-642-76998-6
eBook Packages: Springer Book Archive