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In Vitro Fertilization in Flowering Plants

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Reproductive Biology and Plant Breeding

Abstract

Among the most refractory remaining physiological problems concerning sexual reproduction is the mechanism of interaction between the male and female gametes during fertilization. In Angiosperms, the double fertilization process is dependant on numerous events beginning with the deposition of the pollen grain on the stigmatic surface and ending with the double gametic fusion within the embryo sac. During the last decade a renewed interest in this subject has been triggered as a result of the discovery, through cytological studies, of cytoplasmic dimorphism of sperm cells (Russell 1983). In Plumbago, Russell (1985) has shown the occurence of a targeted fertilization process: One of the two sperm cells which is plastid-rich preferentially fuses with the egg cell and subsequently forms the embryo, the second (mitochondria rich) associates with the central cell to initiate endosperm formation.

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Literature References

  • Chaboud A., Perez R., Digonnet C., Dumas C. (1992) Gamete recognition in Angiosperms: model and strategy analysis.Society for experimental biology. Seminar series 48. Perspectives in plant recognition. J.A.Callow and J.R. Green (eds). In press.

    Google Scholar 

  • Dupuis I., Roeckel P., Matthys-Rochon E., Dumas C. (1987). Procedure toisolate viable sperm cells from corn (Zea mays L.) pollen grains. Plant Physiol., 85: 876–878

    Article  PubMed  CAS  Google Scholar 

  • Faure J.E., Mogensen H. L., Kranz E., Digonnet C., Dumas C. (1992) Ultrastructural characterization and three-dimensional reconstruction of isolated maize (Zea mays L.) egg cells. In preparation.

    Google Scholar 

  • Heslop Harrison J. and Heslop Harrison Y. (1970) Evaluation of pollen viability by enzymatically induced fluorescence, intra cellular hydrolysis of fluoresceine diacetate, Stain Technol. 45: 115–120

    Google Scholar 

  • Keijzer C.J., Reinders M.C., Leferink-ten Klooster H.B. (1988) A micromanipulation method for artificial fertilization in Torenia. In: Cresti M., Gori P., Pacini E.(eds) Sexual reproduction in higher plants. Springer, Berlin Heidelberg New york, pp 119–124

    Google Scholar 

  • Kranz E., Bautor J., Lörz H. (1991a) In vitro fertilization of single, isolated gametes of maize mediated by electrofusion. Sex. Plant Reprod. 4: 12–18

    Google Scholar 

  • Kranz E., Bautor J., Lörz H. (1991b) Electrofusion mediated transmission of cytoplasmic organelles through the in vitro fertilization process, fusion of sperm cells with synergids and central cells, and cell reconstitution in maize. Sex. Plant Reprod. 4: 17–21

    Google Scholar 

  • Kranz E., Lörz H., Digonnet C., Faure J.E. (1992) In vitro fusion of gametes and production of zygotes. Int. Rev. Cyt. In press.

    Google Scholar 

  • Matthys-Rochon, E., Vergne, P., Detchepare, S., Dumas C. (1987) Male germ unit isolation from three tricellular pollen species: Brassica oleracea, Zea mays and Triticum aestivum. Plant Physiol. 83: 464–466

    Article  PubMed  CAS  Google Scholar 

  • Matthys-Rochon E. and C. Dumas (1988) The male germ unit: retrospect and prospects in: H.J.Wilms and C.J. Keijzer (eds) Plant sperm cells as tools for biotechnology Pudoc Wageningen, the Netherlands. pp 51–60

    Google Scholar 

  • Matthys-Rochon E., Digonnet C., Dumas C. (1991) Microinjection of lucifer yellow into embryo sac cells of Zea mays. International symposium on Angiosperm pollen and ovules. Basic and applied aspects. Cômo-Italy

    Google Scholar 

  • Mogensen H.L. (1988) Exclusion of male mitochondria and plastids during syngamy as a basis for maternal inheritance Proc. Natl. Acad. Sci. USA 85: 2594–2597

    Article  CAS  Google Scholar 

  • Russell S.D. (1983) Fertilization in Plumbago zeylanica: gametic fusion and fate of the male cytoplasm. Amer. J. Bot. 70: 416–434

    Article  Google Scholar 

  • Russell S.D. (1985) Preferential fertilization in Plumbago: ultra structural evidence for gamete level recognition in an Angiosperm. Proc. Natl. Acad. Sci. USA 82: 6129–6132

    Article  PubMed  CAS  Google Scholar 

  • Theunis C.H., Pierson E.S., Cresti M. (1991) Isolation of male and female gametes in higher plants. Sex. Plant Reprod. 4: 145154

    Google Scholar 

  • Wagner V.T., Dumas C., Mogensen H.L. (1989a) Morphometric analysis of isolated Zea mays sperm. Journal of Cell Science. 93: 179–184

    Google Scholar 

  • Wagner V.T., Song Y.C., Matthys-Rochon E., Dumas C. (1989b) Observations on the isolated embryo sac of Zea mays. Plant Science. 59: 127–132

    Article  Google Scholar 

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© 1992 Springer-Verlag Berlin Heidelberg

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Matthys-Rochon, E. (1992). In Vitro Fertilization in Flowering Plants. In: Dattée, Y., Dumas, C., Gallais, A. (eds) Reproductive Biology and Plant Breeding. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76998-6_19

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  • DOI: https://doi.org/10.1007/978-3-642-76998-6_19

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-77000-5

  • Online ISBN: 978-3-642-76998-6

  • eBook Packages: Springer Book Archive

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