Abstract
So far, radiolabeled DNA probes have usually been generated by nick-translation [7], by random priming with oligonucleotides [3,4] and by polymerase chain reaction (PCR) [9]. Radiolabeled RNA probes are synthesized by in vitro transcription [8]. DNA hybridization probes are easy to handle, but they are double stranded and therefore lack strand specificity if they are synthesized using the methods mentioned above. In vitro-transcribed RNA probes are strand specific, but to handle these probes requires RNase free conditions which makes work more cumbersome.
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Stürzl, M., Roth, W.K., Viehweger, P., Hofschneider, PH. (1991). Taq DNA Polymerase-Synthesized Single-Stranded DNA Hybridization Probes and their Application in Northern Blotting and in situ Hybridization. In: Rolfs, A., Schumacher, H.C., Marx, P. (eds) PCR Topics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75924-6_6
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DOI: https://doi.org/10.1007/978-3-642-75924-6_6
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