Abstract
Calmodulin is a ubiquitous calcium binding protein (Cheung 1980; Means and Dedman 1980), which in the adult intestinal epithelial cells has been shown to be localized preferentially at the brush border level (Howe et al. 1980; Glenney et al. 1980). In the intestinal epithelial cells, calmodulin functions mainly as a calcium buffer (Glenney and Glenney 1985), activates the actomyosin based contractility system (Mooseker et al. 1983) and interacts with a number of actin binding proteins. These proteins with both actin and calmodulin binding capacities are mainly the 110 kDa protein (Coudrier et al. 1981; Glenney and Glenney 1984; Howe and Mooseker 1983), cal-desmon (Bretscher and Lynch 1985) and fodrin (Glenney et al. 1982; Hirokawa et al. 1983). They have been extensively studied and characterized in the intestinal brush borders of avians (Glenney and Weber 1980; Mooseker 1985) but not in humans. The aim of the present chapter is to provide some current views of the human intestinal development and to attempt a correlation between the developmental pattern of calmodulin and calmodulin binding proteins, and the epithelial differentiation accompanying intestinal ontogenic maturation.
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References
Bretscher A (1984) Smooth muscle caldesmon. Rapid purification and F-actin cross-linking properties. J Biol Chem 259:12873–12880
Bretscher A, Lynch W (1985) Identification and localization of immunoreactive forms of caldesmon in smooth and non muscle cells; a comparison with the distribution of tropomyosin and actinin. J Cell Biol 150:1656–1663
Carboni JM, Shibayama T, Mooseker MS (1986) Brush border assembly: redistribution of the microvillar core protein, 110K during embryogenesis. J Cell Biol 103:112a (abstract)
Chambers C, Grey RD (1979) Development of the structural components of the brush border in absorptive cells of the chick intestine. Cell Tissue Res 204:387–405
Cheung WY (1980) Calmodulin plays a pivotal role in cellular regulation. Science 207:19–27
Colony PC (1983) Successive phases of human fetal intestinal development. In: Kretchmer N, Ninkowski A (eds) Nutritional adaptation of the gastrointestinal tract of the newborn. Raven Press, New York, pp 3–28
Coudrier E, Reggio H, Louvard D (1981) Immunolocalization of the 110 000 molecular weight cytoskeleton protein of intestinal microvilli. J Mol Biol 152:49–66
Coudrier E, Robine S, Huet C, Arpin M, Sahuquillo C, Louvard D (1984) Expression of two structural markers of brush border intestinal mucosa: villin and a membrane glycoprotein (140 Kd), in a human colon carcinoma cell line HT29. J Submicrosc Cytol 16:159–160
Flanagan SD, Yost B (1984) Calmodulin-binding proteins: visualization by (125I) calmodulin overlay on blots quenched with Tween 20 or bovine serum albumin and poly (Ethylene oxide). Anal Biochem 140:510–519
GlenneyJr, Glenney P (1983) Spectrin, fodrin and TW 260/240: a family of related proteins lining the plasma membrane. Cell Motil 3:671–682
GlenneyJrJr, Glenney P (1984) The microvillus 110K Da cytoskeletal protein is an integral membrane protein. Cell 37:743–751
GlenneyJrJr, Glenney P (1985) Comparison of Ca++ regulated events in the intestinal brush border. J Cell Biol 100:754–763
GlenneyJrJr, Weber K (1980) Calmodulin-binding proteins of the microfilaments present in isolated brush borders and microvilli of intestinal epithelial cells. J Biol Chem 255:10551–10554
GlenneyJrJr, Bretscher A, Weber K (1980) Calcium control of the intestinal microvillus cytoskeleton: its implication for the regulation of microfilament organization. Proc Natl Acad Sci USA 77:6458–6462
GlenneyJrJr, Glenney P, Osborn M, Weber K (1982) An F-actin and calmodulin-binding protein from isolated intestinal brush borders has a morphology related to spectrin. Cell 28:843–854
Haffen K, Kedinger M, Lacroix B (1986) Cytodifferentiation of the intestinal villus epithelium. In: Desnuelle P, Sjöström H, Nören O (eds) Molecular and cellular basis of digestion. Elsevier, Amsterdam, pp 311–322
Hauri HP, Quaroni A, Isselbacher KJ (1979) Biogenesis of intestinal plasma membrane: postradiational route and cleavage of sucrase-isomaltase. Proc Natl Acad Sci USA 76:5183–5186
Hirokawa N, Cheney RE, Willard M (1983) Location of a protein of the fodrin-spectrin-TW260/240 family in the mouse intestinal brush border. Cell 32:953–965
Howe CL, Mooseker MS (1983) Characterization of the 110K dalton actin-calmodulin and membrane-binding protein from microvilli of intestinal epithelial cells. J Cell Biol 97:974–985
Howe CL, Mooseker MS, Graves TA (1980) Brush-border calmodulin: a major component of the isolated microvillus core. J Cell Biol 86:916–923
Ishimura K, Jufita H, Ban T, Matsuda H, Sobue K, Kakiuchi S (1984) Immunocytochemical demonstration of caldesmon (a calmodulin, F-actin interacting protein) in smooth muscle fibers and absorptive intestinal cells in the small intestine of the rat. Cell Tissue Res 235:207–209
Kedinger M, Simon PM, Grenier JF, Haffen K (1981) Role of epithelial-mesenchymal interactions in the ontogenesis of intestinal brush border enzymes. Develop Biol 86:339–347
Kedinger M, Haffen K, Simon-Assmann P (1986) Control mechanisms in the ontogenesis of villus cells. In: Desnuelle P, Sjöström H, Nören O (eds) Molecular and cellular basis of digestion. Elsevier, Amsterdam, pp 323–334
Lacroix B, Wolff-Quenot MJ, Haffen K (1984a) Early human hand morphology: an estimation of fetal age. Early Hum Dev 9:127–136
Lacroix B, Kedinger M, Simon-Assmann P, Haffen K (1984b) Early organogenesis of human small intestine: scanning electron microscopy and brush-border enzymology. Gut 25:925–930
Louvard D, Reggio H, Coudrier E (1986) Cell surface asymmetry is a prerequisite for the function of transporting and secreting epithelia. In: Desnuelle P, Sjöström H, Nören O (eds) Molecular and cellular basis of digestion. Elsevier, Amsterdam, pp 25–42
Mackensie NR, Morris B, Morris R (1983) Protein binding to brush borders of enterocytes from the jejunum of the neonatal rat. Biochim Biophys Acta 755:204–209
Mathan M, Moxey PC, Trier JS (1976) Morphogenesis of fetal rat duodenal villi. Am J Anat 146: 73–92
Means AR, DedmanJr (1980) Calmodulin — an intracellular calcium receptor. Nature (Lond) 285:73–77
Mooseker MS (1985) Organization, chemistry and assembly of the cytoskeletal apparatus of the intestinal brush border. Ann Rev Cell Biol 1:209–241
Mooseker MS, Keller TCS, Hirokawa N (1983) In: Brush border membranes — Ciba Foundation Symposium 95. Pitman books Ltd, London, p 195
Ngai PK, Walsh MP (1985) Detection of caldesmon in muscle and non-muscle tissues of the chicken using polyclonal antibodies. Biochem Biophys Res Commun 127:533–539
Robine S, Huet C, Moll R, Sahuquillo-Nerino C, Coudrier E, Zweibaum A, Louvard D (1985) Can villin be used to identify malignant and undifferentiated normal digestive epithelial cells? Proc Natl Acad Sci USA 82:8488–8492
Rochette-Egly C, Daviaud D (1985) Calmodulin binding to nitrocellulose and zetapore membranes during electrophoretic transfer from Polyacrylamide gels. Electrophoresis 6:235–238
Rochette-Egly C, Garaud JC, Kedinger M, Haffen K (1986) Calmodulin in epithelial intestinal cells during rat development. Experientia 42:1043–1046
Rochette-Egly C, Haffen K (1987) Developmental pattern of calmodulin-binding proteins in rat jejunal epithelial cells. Differentiation (in press)
Shibayama T, Carboni T, Mooseker MS (1986) Brush border assembly: distribution of microvillar core proteins during embryogenesis. J Cell Biol 103:112a (abstract)
Shibayama T, Carboni JM, Mooseker MS (1987) Assembly of the intestinal brush border: appearance and redistribution of microvillar core proteins in developing chick enterocytes. J Cell Biol 105:335–344
Skovbjerg H (1982) High molecular weight pro sucrase-isomaltase in human fetal intestine. Pediatr Res 16:948–949
Triadou N, Zweibaum A (1985) Maturation of sucrase-isomaltase complex in human fetal small and large intestine during the gestation. Pediatr Res 19:136–138
Vollrath L (1969) Über die Entwicklung des Dünndarms der Ratte. In: Brodai A, Hild W, Ortman R, Schiebler TH, Tôndury G, Wolff E (eds) Ergebnisse der Anatomie und Entwicklungsgeschichte. Bd 4. Springer, Berlin Heidelberg New York, pp 1 – 66
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Rochette-Egly, C., Lacroix, B., Kedinger, M., Haffen, K. (1988). Calmodulin and Calmodulin Binding Proteins During Differentiation of Human Intestinal Brush Borders. In: Gerday, C., Bolis, L., Gilles, R. (eds) Calcium and Calcium Binding Proteins. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73042-9_13
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DOI: https://doi.org/10.1007/978-3-642-73042-9_13
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