Abstract
The following technique has been developed for synchronisation of virus replication in leaves (Dawson and Schlegel 1973). Inoculated plants are exposed to different temperatures: the lower part with the inoculated leaves to high temperatures (approx. 30 °C), allowing replication of virus, and the upper part to low temperatures (approx. 5 °C), preventing movement of the virus into the upper leaves (temperatures between 5 and 10 °C allowed translocation to top leaves, but were restrictive for replication). After a few days of differential temperature treatment, the plants are transferred to a growth chamber at 25 °C. At that moment (time zero) the “systemic inoculation” starts and the virus enters the vascular system of the top leaves, that is, the phloem of petioles and leaf veins, but cells of the mesophyll do not become infected at the same time. In spite of this lack of synchrony in infection of individual cells, there is relatively synchronous virus replication at the leaf tissue level. Hence, differential temperature treatment is still a valuable technique for studying virus replication and transport.
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References
Dawson WO, Schlegel DE (1973) Differential temperature treatment of plants greatly enhances multiplication rates. Virology 53: 476–478
Dawson WO, Schlegel DE (1976) Synchronization of cowpea chlorotic mottle virus replication in cowpea leaves. Intervirology 7: 284–291
Van Lent JWM, Verduin BJM (1987) Detection of viral antigen in semi-thin sections of plant tissue by immunogold-silver staining and light microscopy. Neth J Plant Pathol 93: 261–272
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© 1998 Springer-Verlag Berlin Heidelberg
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Dijkstra, J., de Jager, C.P. (1998). Differential Temperature Treatment of Infected Plants. In: Practical Plant Virology. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72030-7_3
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DOI: https://doi.org/10.1007/978-3-642-72030-7_3
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-48981-5
Online ISBN: 978-3-642-72030-7
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