Abstract
To obtain protoplasts, leaf tissue is treated with a mixture of pectinases and cellulases to dissolve the middle lamella and cell walls, leading to release of the spherical protoplasts bounded only by the plasma membrane (plasmalemma) (Fig. 1).
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References
Hibi T, Kano H, Sugiura M, Kazami T, Kimura S (1986) High efficiency electrotransfection of tobacco mesophyll protoplasts with tobacco mosaic virus RNA. J Gen Virol 67: 2037–2042
Van Beek NAM, Derksen ACG, Dijkstra J (1985) Polyethylene glycol-mediated infection of cowpea protoplasts with Sonchus yellow net virus. J Gen Virol 66: 551–557
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© 1998 Springer-Verlag Berlin Heidelberg
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Dijkstra, J., de Jager, C.P. (1998). Inoculation of Protoplasts Isolated from Leaves. In: Practical Plant Virology. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72030-7_2
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DOI: https://doi.org/10.1007/978-3-642-72030-7_2
Publisher Name: Springer, Berlin, Heidelberg
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