Skip to main content
SpringerLink
Log in

Consequences of Capacitation. I. Sperm-Egg Binding

  • Chapter
The Epididymis, Sperm Maturation and Fertilisation

  • 162 Accesses

Abstract

Greater numbers of capacitated sperm bind to fertilised eggs than uncapacitated sperm bind to unfertilised eggs (Bedford, 1967; Overstreet and Bedford. 1974) and during this association there is a change from “loose attachment” to a more “firm binding” to the egg surface (Hartmann and Hutchison, 1974a; see Hartmann et al., 1983). It is possible to separate these two events by observing the zona: only bound spermatozoa display lytic activity on the surface and penetrate the zona pellucida (Jedlicki and Barros, 1985), an event intimately linked to the expression of the acrosome reaction (see Section II.D).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ahuja KK (1982) Fertilization studies in the hamster. The role of cell-surface carbohydrate. Exp Cell Res 140: 353–362

    Article  PubMed  CAS  Google Scholar 

  • Ahuja KK (1985) Carbohydrate determinants involved in mammalian fertilization. Am J Anal 174:207–223

    Article  CAS  Google Scholar 

  • Barker LDS and Amann RP (1970) Epididymal physiology. I. Specificity of anlisera against bull spermatozoa and reproductive fluids. J Reprod Fert 22: 441–452

    Article  CAS  Google Scholar 

  • Barker LDS and Amann RP (1971) Epididymal physiology.II. Immunofluorescenl analyses of epithelial secretion and absorption and of bovine sperm maturation. J Reprod Fert 26: 319–332

    Article  CAS  Google Scholar 

  • Bedford JM (1967) The importance of capacitation for establishing contact between eggs and sperm in the rabbit. J Reprod Fert 13: 365–367

    Article  CAS  Google Scholar 

  • Bedford JM (1977) Sperm/Egg interaction: the specificity of human spermatozoa. Anal Rec 188: 477–488

    Article  CAS  Google Scholar 

  • Bedford JM, Cooper GW and Calvin HI (1972) Post-meiotic changes in the nucleus and membranes of mammalian spermatozoa. In: The Genetics of the Spermatozoon. Eds. RA Beatty and S Glucksohn-Waelsh, Bogtrykkeriet Forum, Copenhagen, pp 69–89

    Google Scholar 

  • Bernais A, Torres J, Reyes A and Rosado A (1980) Presence and regional distribution of sialyltransferase in the epididymis of the rat. Biol Reprod 23: 290–293

    Article  Google Scholar 

  • Bleil JD and Wassarman PM (1983) Sperm-egg interaction in the mouse: sequence of events and induction of the acrosome reaction by a zona pellucida glycoprotein. Develop Biol 95: 317–324

    Article  PubMed  CAS  Google Scholar 

  • Brooks DF and Tiver K (1984) Analysis of surface proteins of rat spermatozoa during epididymal transit and identification of antigens common to spermatozoa, rete testis fluid and cauda epididymal plasma. J Reprod Fert 71: 249–257

    Article  CAS  Google Scholar 

  • Brown CR, von Glos KI and Jones R (1983) Changes in plasma membrane glycoproteins of rat spermatozoa during maturation in the epididymis. J Cell Biol 96: 256–264

    Article  PubMed  CAS  Google Scholar 

  • Byers SW, Qasba PK and Paulson HL (1984) Immunocytochemical localization of a-lactalbumin in the male reproductive tract. Biol Reprod 30: 171–177

    Article  PubMed  CAS  Google Scholar 

  • Cooper TG and Orgebin-Crist MC (1975) The effect of epididymal and testicular fluids on the fertilising capacity of testicular and epididymal spermatozoa. Andrologia 7: 85–93

    Article  PubMed  CAS  Google Scholar 

  • Corselli J and Talbot P (1986) An in vitro technique to study penetration of hamster oocytecumulus complexes by using physiological numbers of sperm. Gamete Res 13: 293–308

    Article  Google Scholar 

  • Cossu G and Boitani C (1984) Lactosaminoglycans synthesised by mouse male germ cells are fucosylated by an epididymal fucosy I transferase. Develop Biol 102: 402–408

    Article  PubMed  CAS  Google Scholar 

  • Crozet N and Dumot M (1984) The site of the acrosome reaction during in vivo penetration of the sheep oocyte. Gamete Res 10: 97–105

    Article  Google Scholar 

  • Cuasnicú PS, González Echeverría F, Piazza A and Blaquier JA (1984a) Addition of androgens to cultured hamster epididymis increases zona recognition by immature spermatozoa. J Reprod Pert 70: 541–547

    Article  Google Scholar 

  • Cuasnicú PS, González Echeverría F, Piazza, Piñeiro L and Blaquier JA (1984b) Epididymal proteins that mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa. J Reprod Fert 71: 427–431

    Article  Google Scholar 

  • Cuasnicú PS, Gonzáles Echeverría F, Piazza AL, Cameo MS and Blaquier JA (1984c) Antibodies against epididymal glycoproteins block fertilizing ability in rat. J Reprod Fert 72: 467–471

    Article  Google Scholar 

  • Cummins JM (1976) Effects of epididymal occlusion on sperm maturation in the hamster. J exp Zool 197: 187–190

    Article  Google Scholar 

  • Dacheux JL and Paquignon M (1980) Relations between the fertilizing ability, motility and metabolism of epididymal spermatozoa. Reprod Nutr Develop 20: 1085–1099

    Article  CAS  Google Scholar 

  • Dacheux JL and Paquignon M (1983) Influence of initiation of forward motility on the fertilizing ability of immature boar spermatozoa in in vivo homologous and in vitro heterologous systems of insemination. In: The Sperm Cell Ed. J Andre. Martinus-Nijhoff. The Hague, pp 99–102

    Chapter  Google Scholar 

  • Dacheux JL, Paquignon M and Combarnous Y (1983) Head-to-head agglutination of ram and boar epididymal spermatozoa and evidence for an epididymal antagglutinin. J Reprod Fert 67: 181–189

    Article  CAS  Google Scholar 

  • Durr R, Shur B and Roth S (1977) Sperm-associated sialyltransfcrase activity. Nature (Lond) 265: 547–548

    Article  CAS  Google Scholar 

  • Florman HM and Storey BT (1982) Mouse gamete interactions: the zona pellucida is the site of the acrosome reaction leading to fertilization in vitro. Develop Biol 91: 121–130

    Article  PubMed  CAS  Google Scholar 

  • Florman MM, Saling PM and Storey BT (1983) Sperm/zona pellucida reactions leading to fertilization of mouse eggs in vitro. In: The Sperm Cell. Ed. J Andre. Martinus-Nihjoff. The Hague, pp 111–114

    Chapter  Google Scholar 

  • Fournier-Delpech S, Courtens JL, Pisselet CL, DeLaleu B and Courot M (1982) Acquisition of zona binding by ram spermatozoa during epididymal passage, as revealed by interaction with rat oocytes. Gamete Res 5: 403–408

    Article  Google Scholar 

  • Fournier-Delpech S, Hamamah S, Colas G and Courot M (1983) Aquisition of zona binding structures by ram spermatozoa during epididymal passage. In: The Sperm Cell. Ed. J Andre, Martinus-Nijhoff. The Hague, pp 103–106.

    Google Scholar 

  • Fournier-Delpech S, Hamamah S, Tananis-Anthony C, Courot M and Orgebin-Crist MC (1984a) Hormonal regulation of zona-binding ability and fertilizing ability of rat epididymal spermatozoa. Gamete Res 9: 21–30

    Article  Google Scholar 

  • Fournier-Delpech S, Hamah S, Courot M and Kuntz C (1984b) Androgenic control of zona binding capacity of ram spermatozoa. In: The Male in Farm Animal Reproduction. Ed. M Courot. Martinus-Nijhoff, Boston, pp 103–107

    Google Scholar 

  • Foumier-Delpech S, Courot M and Dubois MP (19S5) Decreased fertility and motility of spermatozoa from rats immunized with a prealbumin epididymal specific glycoprotein. J Androl 6: 246–250

    Google Scholar 

  • González Echeverría FM, Cuasnicú PS and Blaquier JA (1982) Identification of androgen-dependent glycoproteins in the hamster epididymis and their association with spermatozoa. J Reprod Fert 64: 1–7

    Article  Google Scholar 

  • González Echeverría FM, Cuasnicú PS, Piazza A, Pineiro L and Blaquier JA (1984) Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro. J Reprod Fert 71: 432–437

    Google Scholar 

  • Gulyas BI and Schmell ED (1981) Sperm-egg recognition and binding in mammals. In: Bioregulators of Reproduction. Eds. G Jagiello and HJ Vogel, Acad Press. London, pp 499–519

    Google Scholar 

  • Gwatkin RBI and Williams DT (1978) Bovine and hamster zona solutions exhibit receptor activity for capacitated but not for noneapacitated sperm. Gamete Res 1: 259–263

    Article  Google Scholar 

  • Hamilton DW (1980) UDP-galactose: N-acetylglucosamine galactosvltransferasc in fluids from rat rete testis and epididymis. Biol Reprod 23: 377–385

    Article  PubMed  CAS  Google Scholar 

  • Hamilton DW (1981a) Future trends in ccll biological research on the male reproductive system. In: Goals in Male Reproductive Research. Eds. S Boyarsky and K Polakoski, Pergamon Press, Oxford, pp 19–27

    Google Scholar 

  • Hamilton DW (1981b) Evidence for α-lactalbumin-like activity in reproductive tract fluids of the male rat. Biol Reprod 25: 385–392

    Article  PubMed  CAS  Google Scholar 

  • Hamilton DW and Gould RP (1982) Preliminary observations on enzymatic galactosylation of glycoproteins on the surface of rat caput epididymal spermatozoa. Int J Androl Suppl 5: 73–80

    Article  CAS  Google Scholar 

  • Hammerstedt RH, Hay SR and Amann RP (1982) Modification of ram sperm membranes during epididymal transit. Biol Reprod 27: 745–754

    Article  PubMed  CAS  Google Scholar 

  • Hartmann JF (1983) Mammalian fertilization: gamete surface interactions in vitro. In: Mechanism and Control of Animal Fertilization. Ed. JF Hartmann. Acad Press. New York, pp 325–364

    Google Scholar 

  • Hartmann JF and Hutchison CF (1974) Nature of the pre-penetration contact interactions between hamster gametes in vitro. J Reprod Fert 36: 49–57

    Article  CAS  Google Scholar 

  • Hartmann JF and Hutchison CF (1977a) Involvement of two carbohydrate-containing components in the binding of uncapacitated spermatozoa to eggs of the golden hamster in vitro. J exp Zool 201: 383–390

    Article  PubMed  CAS  Google Scholar 

  • Heffner LJ and Storey BT (1982) Cold lability of mouse sperm binding to zona pellucida. J exp Zool 219: 155–161

    Article  PubMed  CAS  Google Scholar 

  • Heffner LJ, Saling PM and Storey BT (1980) Separation of calcium effects on motility and zona binding ability in mouse spermatozoa. J exp Zool 212: 53–57

    Article  PubMed  CAS  Google Scholar 

  • Hoppe PC (1975) Fertilizing ability of mouse sperm from different epididymal regions and after washing and centrifugation. J exp Zool 192: 219–222

    Article  PubMed  CAS  Google Scholar 

  • Huang TTF and Yanagimachi R (1982) Some factors controlling attachment of guinea pig spermatozoa to zona pellucida. Proc Japan Acad 58B: 101–104

    Article  CAS  Google Scholar 

  • Huang TTF and Yanagimachi R (1984) Fucoidan inhibits attachment of guinea pig spermatozoa to the zona pellucida through binding to the inner acrosomal membrane and equatorial domains. Exp Cell Res 153: 363–373

    Article  PubMed  CAS  Google Scholar 

  • Huang TTF, Fleming AD and Yanagimachi R (1981) Only acrosome-reacted spermatozoa can bind to and penetrate the zona pellucida: a study using the guinea pig. J exp Zool 217: 287–290

    Article  PubMed  CAS  Google Scholar 

  • Huang TTF, Ohzu E and Yanagimachi R (1982) Evidence suggesting that L-fucose is part of a recognition signal for sperm-zona pellucida attachment in mammals. Gamete Res 5: 355–361

    Article  CAS  Google Scholar 

  • Jedlicki A and Barros C (1985) Scanning electron microscope study of on vitro prepenetration gamete interactions. Gamete Res 11: 121–131

    Article  Google Scholar 

  • Ji TH and Nicolson GL (1974) Lectin binding and perturbation of the outer surface of the cell membrane induces a transmembrane organizational alteration at the inner surfacc. Proc Natn Acad Sci US 71: 2212–2216

    Article  CAS  Google Scholar 

  • Jones R and Brown CR (1982) Association of epididymal secretory proteins showing α-lactalbumin-like activity with the plasma membrane of rat spermatozoa. Biochem J 206: 161–164

    PubMed  CAS  Google Scholar 

  • Jones R, Pholpramool C, Setchell BP and Brown CR (1981) Labelling of membrane glycoproteins in rat spermatozoa collected from different regions of the epididymis. Biochem J 200: 457–460

    PubMed  CAS  Google Scholar 

  • Klinefelter GR and Hamilton DW (1984) Organ culture of rat caput epididymal tubules in a perifusion chamber. J Androl 5: 243–258

    PubMed  CAS  Google Scholar 

  • Kohane AC, Cameo MS, Pineiro L, Garberi JC and Blaquier JA (1980) Distribution and site of production of specific proteins in the rat epididymis. Biol Reprod 23: 181–187

    Article  PubMed  CAS  Google Scholar 

  • Kuzan F, Fleming A and Seidel G (1984) Successful fertilization in vitro of fresh oocytes by perivitelline (acrosome-reacted) spermatozoa of the rabbit. Fert Steril 41: 766–770

    CAS  Google Scholar 

  • Lambert H (1984) Role of sperm-surface glycoproteins in gamete recognition in two mouse species. J Reprod Fert 40: 281–284

    Article  Google Scholar 

  • Lambert H and vanLe A (1984) Possible involvement of a sialylated component of the sperm plasma membrane in the sperm-zona interaction in the mouse. Gamete Res 10: 153–163

    Article  CAS  Google Scholar 

  • Lambiase JT and Amann RP (1973) Infertility of rabbit testicular spermatozoa collected in their native environment. Fert Steril 24: 65–67

    Google Scholar 

  • Lee MA and Storey NT (1985) Evidence for plasma membrane impermeability to small ions in acrosome-intact mouse spermatozoa bound to mouse zonae pellucidae, using an amino-acridine fluorescent pH probe; time course of the zona-induced acrosome reaction monitored by both chlortetracycline and pH probe fluorescence. Biol Reprod 33: 235–246

    Article  PubMed  CAS  Google Scholar 

  • Letts PJ, Meistrich ML, Bruce WR and Schacter H (1974) Glycoprotein glycosyl-transferase levels during spermatogenesis in mice. Biochem Biophys Acta 343: 192–207

    Article  PubMed  CAS  Google Scholar 

  • Lopez LC, Bayna EM, Litoff D, Shaper NL, Shaper JH and Shur BD (1985) Receptor function of mouse sperm surface galactosyltransferase during fertilization. J Cell Biol 101: 1501–1510

    Article  PubMed  CAS  Google Scholar 

  • Matityahu A, Shalgi R and Nebel L (1984) The role of carbohydrates in sperm-egg attachment in rats. Am J Reprod Immunol 6: 60 (abst).

    Google Scholar 

  • Moore HDM (1979) The net surface charge of mammalian spermatozoa as determined by isoelectric focussing. Changes following sperm maturation, ejaculation, incubation in the female tract, and after enzyme treatment. Int J Androl 2: 449–462

    Article  CAS  Google Scholar 

  • Moore HDM (1981) Effects of castration on specific glycoprotein secretions of the epididymis in the rabbit and hamster. J Reprod Fert 61: 347–354

    Article  CAS  Google Scholar 

  • Nicolson GJ and Yanagimachi R (1979) Cell surface changes associated with the epididymal maturation of mammalian spermatozoa In: The Spermatozoon. Maturation. Motility, Surface Properties and Comparative Aspects. Eds. DW Fawcett and JM Bedford. Urban and Schwarzenberg. Baltimore-Munich, pp 187–194

    Google Scholar 

  • Olson GE and Hamilton DW (1978) Characterization of the surface glycoproteins of rat spermatozoa. Biol Reprod 19:26–35

    Article  PubMed  CAS  Google Scholar 

  • Olson GE and Orgebin-Crist MC (1982) Sperm surface changes during epididymal maturation. Ann NY Acad Sci 383: 372–391

    Article  PubMed  CAS  Google Scholar 

  • Orgebin-Crist MC and Fournier-Delpech S (1982) Sperm-egg interactions. Evidence for maturational changes during epididymal transit. J Androl 3: 429–433

    Google Scholar 

  • Overstreet JW and Bedford JM (1974) Importance of sperm capacitation for gamete contact in the rabbit. J Reprod Fert 39: 393–398

    Article  CAS  Google Scholar 

  • Peterson RN, Russell L, Bundman D and Freund M (1979) Sperm-egg interaction: evidence for boar sperm plasma membrane receptors for porcine zona pellucida. Nature (Lond) 207: 73–74

    Google Scholar 

  • Peterson RN, Russell LD, Bundman D, Conway M and Freund M (1981) The interaction of living boar sperm and sperm plasma membrane vesicles with the porcine zona pellucida. Develop Biol 84: 144–156

    Article  PubMed  CAS  Google Scholar 

  • Peterson RN, Robl JM, Dziuk PJ and Russell LD (1982) The effects of antisperm plasma membrane antibodies on sperm-egg binding, penetration and fertilization in the pig. J exp Zool 223: 79–81

    Article  PubMed  CAS  Google Scholar 

  • Peterson RN, Russell LD, and Hunt WP (1984) Evidence for specific binding of uncapacitated boar spermatozoa to porcine zonae pellucidae in vitro. J exp Zool 231: 137–147

    Article  PubMed  CAS  Google Scholar 

  • Peterson RN, Henry L, Hunt W, Saxena N and Russell LD (1985) Further characterization of boar sperm plasma membrane proteins with affinity for the porcine zona pellucida. Gamete Res 12: 91–100

    Article  CAS  Google Scholar 

  • Pierce M, Turley EA and Roth R (1980) Cell surface galactosyltransferase activities. Int Rev Cytol 65: 1–47

    Article  PubMed  CAS  Google Scholar 

  • Roth S (1973) A molecular model for cell interactions. Quart Rev Biol 48: 541–563

    Article  PubMed  CAS  Google Scholar 

  • Saling PM (1981) Involvement of trypsin-like activity in binding of mouse spermatozoa to zonae pellucidae. Proc Nat Acad Sci US 78: 6231–6235

    Article  CAS  Google Scholar 

  • Saling PM (1982) Development of the ability to hind to zonae pellucidae during epididymal maturation: reversible immobilisation of mouse spermatozoa by lanthanum. Biol Reprod 26: 429–436

    Article  PubMed  CAS  Google Scholar 

  • Saling PM and Storey BT (1979) Mouse gamete interactions during fertilization in vitro: Chlortetracycline as fluorescent probe for the sperm acrosome reaction. J Cell Biol 83: 544–555

    Article  PubMed  CAS  Google Scholar 

  • Saling PM, Storey BT and Wolf DP (1978) Calcium-dependent binding of mouse epididymal spermatozoa to the zona pellucida. Develop Biol 65: 515–525

    Article  PubMed  CAS  Google Scholar 

  • Saling PM, Sowinski J and Storey BT (1979) An ultrastructural study of epididymal mouse spermatozoa binding to zonae pellucidae in vitro: sequential relationship to the acrosome reaction. J exp Zool 209: 229–238

    Article  PubMed  CAS  Google Scholar 

  • Shur BD (1981) Galactosyltransferase activities on mouse sperm bearing multiple t lethal and t viable haplotypes of the T/t-complex. Genet Res Cambridge 38: 225–236

    Article  CAS  Google Scholar 

  • Shur BD and Bennett D (1979) A specific defect in galactosyltransferase regulation on sperm bearing mutant alleles of the T/t locus. Develop Biol 71: 243–259

    Article  PubMed  CAS  Google Scholar 

  • Schur BD and Hall NG (1982a) Sperm surface galactosyltransferase activities during in vitro capacitation. J Cell Biol 95: 567–573

    Article  Google Scholar 

  • Shur BD and Hall NG (1982b) A role for mouse sperm surface galactosyltransferase in sperm binding to the egg zona pellucida. J Cell Biol 95: 574–579

    Article  PubMed  CAS  Google Scholar 

  • Storey BT, Lee MA, Muller C, Ward CR and Wirtshafter DG (1984) Binding of mouse spermatozoa to the zona pellucida of mouse eggs in cumulus: evidence that the acrosome remains substantially intact. Biol Reprod 31: 1119–1128

    Article  PubMed  CAS  Google Scholar 

  • Suarez SS, Katz DF and Meizel S (1984) Changes in motility that accompany the acrosome reaction in hyperactivated hamster spermatozoa. Gamete Res 10: 253–265

    Article  Google Scholar 

  • Swenson CE and Dunbar RS (1982) Specificity of sperm-zona interaction. J exp Zool 219: 97–104

    Article  PubMed  CAS  Google Scholar 

  • Tadolini B, Wilson J, Reddy PRK and Williams-Ashman HG (1977) Characteristics and hormonal control of some glycoprotein glycosyltransferase reactions in male reproductive organs. Advances in Enzyme Regulation. Ed. G Weber. Volume 15. pp 319–336

    Google Scholar 

  • Talbot P (1985) Sperm penetration through oocyte investments in mammals. Am J Anat 174: 331–346

    Article  PubMed  CAS  Google Scholar 

  • Toowicharanont P and Chulavatnatol M (1983) Characterization of sialoglycoproteins of rat epididymal fluid and spermatozoa by periodate-tritiated borohydride. J Reprod Fert 67: 113–141

    Article  Google Scholar 

  • Vernon RB, Mueller CH, Herr JC, Feuchter FA and Eddy EM (1982) Epididymal secretion of a mouse sperm surface component recognized by a monoclonal antibody. Biol Reprod 26: 523–535

    Article  PubMed  CAS  Google Scholar 

  • Vierula M and Rajaniemi H (1982) Epididymal maturation of the surface protein structure of mammalian spermatozoa. Medical Biology 60: 323–327

    PubMed  CAS  Google Scholar 

  • Yanagimachi R (1977) Specificity of sperm-egg interaction. In: Immunobiology of Gametes. Eds. M Edinin and MH Johnson. CUP Cambridge, pp 255–289

    Google Scholar 

  • Yanagimachi R (1978) Sperm-Egg Association in Mammals. In: Current Topics in Developmental Biology. Volume 12 Fertilization. Eds. AA Moscona and A Monroy. Academic Press. New York, pp 83–105

    Chapter  Google Scholar 

  • Yanagimachi R (1982) Requirement of extracellular calcium ions for various stages of fertilization and fertilization-related phenomena in the hamster. Gamete Res 5: 323–344

    Article  CAS  Google Scholar 

  • Yanagimachi R, Huang TTF, Fleming AD, Kosower NS and Nicolson GL (1983) Dithiothreitol, a disulphide-reducing agent, inhibits capacitation, acrosome reaction and interaction with eggs by guinea pig spermatozoa. Gamete Res 7: 145–154

    Article  CAS  Google Scholar 

  • Zeheb R and Orr GA (1984) Characterization of a maturation-associated glycoprotein on the plasma membrane of rat caudal epididymal sperm. J Biol Chem 259: 839–848

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V., Klinische Forschungsgruppe für Reproduktionsmedizin an der Frauenklinik der Universität Münster, Steinfurter Straße 107, 4400, Münster, Germany

    Dr. Trevor G. Cooper

Authors
  1. Dr. Trevor G. Cooper
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1986 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Cooper, T.G. (1986). Consequences of Capacitation. I. Sperm-Egg Binding. In: The Epididymis, Sperm Maturation and Fertilisation. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71471-9_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-71471-9_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71473-3

  • Online ISBN: 978-3-642-71471-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation