Abstract
Complementation of yeast cdc and cln mutants as well as biochemical approaches have been used to identify and characterize the major components of the mammalian cell cycle machinery, the cyclins and cyclin-dependent kinases (cdks). More recently the two-hybrid system (or interaction trap) has been used to identify novel regulators of the cell cycle in which a known component of the cell cycle is used as “bait” to search for protein interactors (see chapter by Fiore et al., Part Four). For example, the latter approach was used to identify p21Cip/Waf1/Sdi1/Picl, a small molecular weight inhibitor of cdks (Harper et al. 1993), Cdi1, a dual-specificity phosphatase that interacts with both Cdc2 and Cdk2 (Gyuris et al. 1993; Hannon et al. 1994), and p16Ink4/Mts1, a Cdk4 inhibitor (Serrano et al. 1993).
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© 1996 Springer-Verlag Berlin Heidelberg
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Berlin, V., Chiu, M.I. (1996). Identification of Novel Cell Cycle Targets Using Small Molecule Ligands. In: Pagano, M. (eds) Cell Cycle — Materials and Methods. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57783-3_13
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DOI: https://doi.org/10.1007/978-3-642-57783-3_13
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