Abstract
It is undoubtedly admitted that the study of the cell cycle and yeast genetics cannot be dissociated from each other. Both the fission yeast Schizosaccharomyces pombe and the budding yeast Saccharomyces cerevisiae are powerful genetic systems to investigate the life cycle in broad terms. In both organisms, a large collection of cell division cycle (cdc) mutants that arrest the cell cycle at a specific point have been isolated. Their characterization has contributed to the elucidation of the sequential events of the cell cycle and to the identification of genes whose activity is required for the progression through the cell cycle. The general principle is that the succession of each cell cycle step is dependent on the completion of the preceding step.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Alfa C, Fantes P, Hyams J, McLeod M, Warbrick E (1993) Experiments with fission yeast: a laboratory course manual. Laboratory Press, Cold Spring Harbor
Allshire RC (1990) Introduction of large linear minichromosomes into Schizosaccharomyces pombe by an improved transformation procedure. Proc Natl Acad Sci USA, 87: 4043–4047
Apolinaro E, Nocero M, Jin M, Hoffman CS (1993) Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies. Curr Genet 24:491–495
Barbet N, Muriel WJ, Carr AM (1992) Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114:59–66
Basi, G, Schmid E, Maundrell K (1993) TATA box mutations in the Schizosaccharomy ces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility. Gene 123:131–136
Beach D, Nurse P (1981) High-frequency transformation of the fission yeast Schizosaccharomyces pombe. Nature (London) 290:140–142
Booher R, Beach D (1987) Interaction between cdc13 + and cdc2 + in the control of mitosis in fission yeast; dissociation of the G1 and G2 roles of the cdc2 + protein kinase. EMBO J 6:3441–3447
Booher R, Beach D (1988) Involvement of cdc13 + in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules. EMBO J 7: 2321–2327
Bröker M (1987) Transformation of intact schizosaccharomyces pombe cells with plasmid DNA. BioTechniques7 5:516–518
Bröker M (1993) Rapid transformation of cryopreserved competent Schizosaccharomyces pombe cells. BioFeedback 15:599–600
Bröker M, Bαuml O (1989) New expression vectors for the fission yeast Schizosaccharomyces pombe. FEBS Letter 248:105–110
Cottarel G, Beach D, Deuschle U (1993) Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2. Curr Genet 23:547–548
Cottarel G (1995) The Saccharomyces cerevisiae HIS3 and LYS2 genes complement the Schizosacchaformyces pombe his5-303 and lysl-131 mutations, respectively: new selectable markers and multi-purpose multicopy shuttle vectors pSP3 and pSP4. Curr Genet (in press)
Ekwall K, Ruusala T (1991) Budding yeast CAN1 gene as a selection marker in fission yeast. Nucleic Acid Res 19:1150
Elble R (1992) A simple and efficient procedure for transformation of yeasts. BioTechniques 13:18–20
Faryar K, Gatz C (1992) Construction of a tetracycline-inducible promoter in Schizosaccharomyces pombe. Curr Genet 21: 345–349
Forsburg SL (1993) Comparison of Schizosaccharomyces pombe expression system. Nucleic Acid Res 21:2955–2956
Hoffman CS (1993) Preparations of yeast DNA, RNA and proteins. In: Current protocols in molecular biology. Edited by Ausubel, Brent, Kingston, Moore, Seidman, Smith and Struhl.
Hoffman CS, Winston F (1989) A transcriptionally regulated expression vector for the fission yeast Schizosaccharomyces pombe. Gene 84:473–479
Hood MT, Stachow C (1990) Transformation of Schizosaccharomyces pombe by electroporation. Nucleic Acid Res 18:688
Jones RH, Moreno S, Nurse P, Jones NC (1988) Expression of the SV40 promoter in fission yeast: identification and characterization of an AP-1 like factor. Cell 53:659–667
Kanter-Smoler G, Dahlkvist A, Sunnerhagen P (1994) Improved method for rapid transformation of intact Schizosaccharomyces pombe cells. BioTechniques 16:798–799
Lee MG, Nurse P (1987) Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2. Nature London 327:31–35
Lohka MJ, Hayes MK, Maller JL (1988) Purification of maturation-promoting factor, an essential regulator of early mitotic event. Proc Natl Acad Sci USA 85:3009–3013
Matsumoto T, Beach D (1991) Premature initiation of mitosis in yeast lacking RCC1 or an interacting GTPase. Cell 66:347–360
Maundrell K (1990) nmt1 of fission yeast a highly transcribed gene completely repressed by thiamine. J Biol Chem 265:10857–10864
Maundrell K (1993) Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene 123:127–130
McLeod M, Stein M, Beach, D (1987) The product of the mei3 + gene, expressed under the control of the mating-type locus, induces meiosis and sporulation in fission yeast. EMBO J 6:729–736
Molz L, Booher R, Young P, Beach D (1989) cdc2 and the regulation of mitosis: six interacting mcs genes. Genetics 122: 773–782
Moreno S, Klar A, Nurse P (1991) A guide to yeast genetics and molecular biology. Methods Enzymol 194:795–823
Nurse P (1975) Genetic control of cell size at cell division in yeast. Nature (London), 256:547–551
Okazaki K, Okazaki N, Kume K, Jinno S, Tanaka K, Okayama H (1990) High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acid Res 18: 6485–6489
Picard D, Schena M, Yamamoto KR (1990) An inducible expression vector for both fission and budding yeast. Gene 86:257–261
Prentice HL (1992) High efficiency transformation of Schizosaccharomyces pombe by electroporation. Nucleic Acid Res 20:621
Rusell P, Nurse P (1986) cdc25+ functions as an inducer in the mitotic control of fission yeast. Cell 45:145–153
Weilguny D, Praetorius M, Carr A, Egel R, Nielsen O (1991) New vectors in fission yeast: application for cloning the his2 gene. Gene 99:47–54
Xiong YT, Connolly B, Futcher B, Beach D (1991) Human D-type cyclin. Cell 65:691–699
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Cottarel, G. (1996). Cloning and Expression of Cell Cycle Genes in Schizosaccharomyces pombe . In: Pagano, M. (eds) Cell Cycle — Materials and Methods. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57783-3_11
Download citation
DOI: https://doi.org/10.1007/978-3-642-57783-3_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-58066-9
Online ISBN: 978-3-642-57783-3
eBook Packages: Springer Book Archive