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The Seminal Contributions of Gregorio Weber to Modern Fluorescence Spectroscopy

  • Chapter
New Trends in Fluorescence Spectroscopy

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 1))

Abstract

Gregorio Weber is acknowledged to be the person responsible for many of the more important theoretical and experimental developments in modern fluorescence spectroscopy In particular, Weber pioneered the application of fluorescence spectroscopy to the biological sciences. His list of achievements includes:

  • The synthesis and use of dansyl chloride as a probe of protein hydrodynamics

  • The extension of Perrin’s theory of fluorescence polarization to fluorophores associated with random orientations with ellipsoids of revolution and to mixtures of fluorophores

  • The first spectral resolution of the fluorescence of the aromatic amino acids and of intrinsic fluorescence of proteins

  • The first demonstration that both FAD and NADH make internal complexes

  • The first report on aromatic secondary amines, which are strongly fluorescent in apolar solvents, but hardly in water, the most spectacular case being the anilino-naphthalene sulfonates (ANS)

  • The first description of the use of the fluorescence of small molecules as probes for the viscosity of micelles, with implications for membrane systems

  • A general formulation of depolarization by energy transfer

  • The discovery of the “red-edge” effect in homo-energy transfer

  • The development of modern cross-correlation phase fluorometry

  • The development of the excitation-emission matrix method for resolving contributions from multiple fluorophores

  • The synthesis of several novel fluorophores, including pyrenebutyric acid, IAEDANS, bis- ANS, PRODAN, and LAURDAN, designed to probe dynamic aspects of biomolecules

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  1. D. M. Jameson
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Editors and Affiliations

  1. Conservatoire National des Arts et Métiers, Laboratoire de Chimie Général, 292 rue Saint-Martin, 75141, Paris Cedex 03, France

    Bernard Valeur

  2. Ecole Normale Supérieure de Cachan, 61 Av. du Président Wilson, 94235, Cachan Cedex, France

    Jean-Claude Brochon

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Jameson, D.M. (2001). The Seminal Contributions of Gregorio Weber to Modern Fluorescence Spectroscopy. In: Valeur, B., Brochon, JC. (eds) New Trends in Fluorescence Spectroscopy. Springer Series on Fluorescence, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56853-4_3

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