Abstract
Genomic science has now advanced to the point where it is possible to define the genomic structure and gene content of any organism. Such advances have led to the development of expression tools that can study gene expression in a massively parallel fashion. These methods are also affordable, sensitive, discriminating, and require minimal sample RNA. cDNA microarray expression profiling is a rapidly developing technology that makes possible monitoring of gene expression on a genome-wide scale.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Alizadeh AA, Eisen MB, Davis RE, Ma C, Lossos IS, Rosenwald A, Boldrick JC, Sabet H, Tran T, Yu X, Powell JI, Yang L, Marti GE, Moore T, Hudson J Jr, Lu L, Lewis DB, Tibshirani R, Sherlock G, Chan WC, Greiner TC, Weisenburger DD, Armitage JO, Warnke R, Staudt LM (2000) Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature 403(6769):503–511
Bonaldo MF, Lennon G, Soares MB (1996) Normalization and subtraction: two approaches to facilitate gene discovery. Genome Res 6(9):791–806
DeRisi J, Penland L, Brown PO, Bittner ML, Meitzer PS, Ray M, Chen Y, Su YA, Trent JM (1996) Use of a cDNA microarray to analyse gene expression patterns in human cancer. Nat Genet 14(4):457–460
DeRisi JL, Iyer VR, Brown PO (1997) Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278:680–686
Dixon AK, Richardson PJ, Lee K, Carter NP, Freeman TC (1998) Expression profiling of single cells using 3 prime end amplification (TPEA) PCR. Nucleic Acids Res 26(19):4426–4431
Eisen MB, Spellman PT, Brown PO, Botstein D (1998) Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sei USA 95(25): 14863–14868
Grimmond S, Van Hateren N, Siggers P, Arkell R, Larder R, Soares MB, Bonaldo M, Smith L, Tymowska-Lalanne Z, Wells C, Greenfield A (2000) Sexually dimorphic expression of protease nexin-1 and Vanin-1 in the developing mouse gonad prior to overt differentiation suggests a role in mammalian sexual development Hum Mol Genet 9(10):1553–1560
Hegde P, Qi R, Abernathy K, Gay C, Dharap S, Gaspard R, Earle-Hughes J, Snesrud E, Lee N, Quackenbush J (2000) A concise guide to cDNA microarray Anal Biotech 29(3):548–562
Kacharmina JE, Crino PB, Eberwine J (1999) Preparation of cDNA from single cells and subcellular regions. Methods Enzymol 303:3–18
Luo L, Salunga RC, Guo H, Bittner A, Joy KC, Galindo JE, Xiao H, Rogers KE, Wan JS, Jackson MR, Erlander MG (1999) Gene expression profiles of laser-captured adjacent neuronal subtypes. Nat Med 5(l):117–122
Schena M, Shalon D, Davis RW, Brown PO (1995) Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270:467–470
Schena M, Shalon D, Heller R, Chai A, Brown PO, Davis RW (1996) Parallel human genome analysis: microarray-based expression monitoring of 1000 genes. Proc Natl Acad Sei USA 93:10614–10619
Soares MB, Bonaldo MF, Jelene P, Su L, Lawton L, Efstratiadis A (1994) Construction and characterization of a normalized cDNA library. Proc Natl Acad Sei USA 91(20):9228–9232
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Grimmond, S., Greenfield, A. (2001). Expression Profiling with cDNA Microarrays: A User’s Perspective and Guide. In: Jordan, B.R. (eds) DNA Microarrays: Gene Expression Applications. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56517-5_2
Download citation
DOI: https://doi.org/10.1007/978-3-642-56517-5_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-41508-4
Online ISBN: 978-3-642-56517-5
eBook Packages: Springer Book Archive