Summary
Inhibition of ecto-5′-nucleotidase with an antiserum failed to inhibit adenosine formation in rat polymorphonuclear leucocytes or neonatal heart cells in culture. Adenosine accumulated in the cytoplasm and was exported by the dipyridamole-sensitive nucleoside transporter. Adenosine formation in polymorphonuclear leucocytes was accounted for by the activity of the cytoplasmic 5′-nucleotidase, the low activity of which in rat erythrocytes was associated with reduced capacity to hydrolyse AMP. Globally-ischaemic pigeon hearts produced adenosine rapidly despite the virtual absence of ecto-5′-nucleotidase activity. An ATP-activated 5′-nucleotidase, sufficiently active to account for adenosine fourmation, was present in the soluble fraction of pigeon ventricle. The data suggest that a cytoplasmic 5′-nucleotidase provides the biochemical link between cellular ATP breakdown and adenosine formation.
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© 1987 Springer-Verlag Berlin Heidelberg
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Newby, A.C., Worku, Y., Meghji, P. (1987). Critical Evaluation of the Role of Ecto- and Cytosolic 5′-Nucleotidase in Adenosine Formation. In: Gerlach, E., Becker, B.F. (eds) Topics and Perspectives in Adenosine Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-45619-0_13
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DOI: https://doi.org/10.1007/978-3-642-45619-0_13
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