Abstract
The field of morphometrics has transitioned relatively smoothly through several different phases, from D’Arcy Thompson’s (1917) extraordinary and influential treatise on growth and form, through the influx of algebraic and statistical methods related to eigenanalysis, cluster analysis, and multidimensional scaling, to direct landmark-based Procrustes and deformation methods that echo Thompson’s original intents and insights. In a sense, the discipline is still riding the wave of methodological advances that began in the 1970s (Adams et al. 2004). Although it is difficult to predict to direction of future methodological advances, it is certain that morphometric methods will be extended to areas currently at the periphery of current applications, such as the use of morphometrics to study the effects of quantitative trait loci (Klingenberg et al. 2001; Klingenberg 2003; Leamy et al. 2008) and the sizes and shapes of molecules (Billoud et al. 2000; Bookstein 2004; Rogen and Bohr 2003). However, even given the current level of methodological sophistication, there are still some important technical and conceptual problems to be solved in the shorter term. I will briefly highlight just a few of these here.
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Keywords
- Quantitative Trait Locus
- Geometric Morphometrics
- Centroid Size
- Morphometric Method
- Geometric Morphometric Analysis
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1 Idea and Aims
The field of morphometrics has transitioned relatively smoothly through several different phases, from D’Arcy Thompson’s (1917) extraordinary and influential treatise on growth and form, through the influx of algebraic and statistical methods related to eigenanalysis, cluster analysis, and multidimensional scaling, to direct landmark-based Procrustes and deformation methods that echo Thompson’s original intents and insights. In a sense, the discipline is still riding the wave of methodological advances that began in the 1970s (Adams et al. 2004). Although it is difficult to predict to direction of future methodological advances, it is certain that morphometric methods will be extended to areas currently at the periphery of current applications, such as the use of morphometrics to study the effects of quantitative trait loci (Klingenberg et al. 2001; Klingenberg 2003; Leamy et al. 2008) and the sizes and shapes of molecules (Billoud et al. 2000; Bookstein 2004; Rogen and Bohr 2003). However, even given the current level of methodological sophistication, there are still some important technical and conceptual problems to be solved in the shorter term. I will briefly highlight just a few of these here.
2 Three-Dimensional Analyses
The extension from 2-dimensional to 3-dimensional analyses has been available in principle for many years, and the use of 3D landmarks is becoming standard practice in fields such as physical anthropology and biomedical science. Procrustes methods are easily extended to three (or more) dimensions, and analyses of Procrustes residuals (Berge and Penin 2004; Lockwood et al. 2002; Nicholson and Harvati 2006) and, more recently, application of three-dimensional extensions of thin-plate splines and other morphometric methods are becoming more widely used (Bookstein 1989; Gunz et al. 2005; Mitteroecker et al. 2004; Mitteroecker and Gunz 2002; O’Higgins and Jones 1998; Rohlf and Bookstein 2003; Rohr 2001).
Because illustrations of 3D thin-plate splines are rare, a simple example is illustrated in Fig. 16.1. This “thin-hyperplate” spline corresponds to the distortion of a malleable cube. The shift of any arbitrary point is given by a weighted sum of landmark shifts, in which each landmark shift is weighted according to its distance from the point (Bookstein 1989). The weights for interpolated shifts of arbitrary points in three dimensions are \(-U(d)=-\vert d \vert \), where d is the distance from the point to a landmark. The stacked 2D planar grids for the reference form are a sample of slices through what is actually a solid 3D grid, but the planar grids are useful for visualization. The corresponding layers in the final deformed “cube” are, of course, not necessarily planar even for subsets of landmarks that lie in a plane in both the reference and target forms. Although, in parallel to the 2D case, the non-affine component of the spline can be decomposed into eigenfunctions in the 3D case, there are a number of computational details that need to be resolved. Bookstein (1989) listed several aspects of the 3D extension that “will require considerable imagination”. In particular, since 2D splines are visualized in 3D, it is not clear how best to draw a thin-hyperplane spline that is a 3D projection of a 4D geometric object.
3 Landmark Variation
The problem of how to adequately characterize relative amounts and directions of variation at individual landmarks is of interest to many researchers, particularly those utilizing Procrustes analyses of 3D coordinates. Results depend sensitively on the particular method used to superimpose configurations of landmarks (the so-called registration problem). Although several versions of Procrustes alignment are available and their statistical properties have been well characterized (Rohlf 1990; Rohlf and Slice 1990), there are no apparent biological rationales for choosing among them. Minimizing the sum-of-squared deviations among corresponding landmarks on different forms tends to produce circular distributions of coordinate positions, with approximately equal amounts of dispersion for different landmarks, while minimizing the median deviations tends to produce more elliptical distributions and more variation among landmarks. Currently these statistical patterns cannot be distinguished from inherent biological variability.
4 Allometry
More direct ties to developmental biology are needed (Gilbert 2003; Klingenberg 2002), particularly with respect to allometric scaling. The use of power functions to characterize scaling relationships (Huxley 1932) has a long and venerable history (Brown et al. 2000; Strauss 1993), and in most biological disciplines the term “allometry” is virtually synonymous with use of power functions (and, more recently, with fractal dimensions). In the context of geometric morphometrics, however, shape is defined much more generally as the “geometric information that remains when location, scale and rotational effects are filtered out from an object” (Bookstein 1978; Kendall 1977, etc.). The Procrustes method is used in geometric morphometrics to standardize forms to a common centroid size, which represents an isometric size standardization (Bookstein 1991; Rohlf 1993). Consequently, the concept of allometry has been generalized to any variation in shape that is correlated with size (Bookstein 1991; Zelditch et al. 2003). The null hypothesis both for the geometric model and for Huxley’s model is isometry; however, deviation from the null in Huxley’s model represents a particularly constrained form of anisometry. In geometric morphometric analyses the coefficients describing shape differences are not meaningful in terms of specific growth models such as Huxley’s power law (Zelditch et al. 2004). Whereas deformation models are portrayed in the linear space, Huxley’s model is linear in the log-space. The anisometric “shape” variation studied in geometric morphometrics therefore consists of two components: allometric (sensu Huxley) and non-allometric. The issue of allometric size-adjustment (sensu Huxley) of Procrustes residuals or of deformation grids needs to be pursued further (e.g., Hammer 2004).
5 Missing Data
Missing data are a frequent problem in morphometrics, as they are generally in multivariate statistical analyses (Reig 1998; Richtsmeier et al. 1992; Strauss et al. 2003; Strauss and Atanassov 2006; Yaroch 1996). If a form is distorted (as in fossils) or has been damaged or broken off (as in delicate skeletal samples), then landmarks can be missing in some specimens. In statistical studies there are two main strategies for dealing with missing data: either the variables with missing data (coordinates of a missing landmark, in this case) must be ignored, or missing values must be imputed (i.e., estimated from the values in complete specimens). Gunz et al. (2002) have summarized how knowledge about the context of missing landmarks can be used to approximate their positions. This would include factors such as bilateral symmetry (landmarks on one side of the body can be “mirrored” to the other side), allometry (regression of Procrustes shape coordinates on centroid size), morphological integration (quantifying patterns of covariation of subsets of landmarks), and curvature smoothness (as quantified by magnitudes of deformation associated with the thin-plate spline interpolation). However, only a few preliminary comparative studies of these different approaches have been carried out, and much additional work needs to be done to characterize the best strategies for dealing with missing data.
6 Phylogenetics
Because morphometric studies are often carried out within a phylogenetic context, the use of morphometric data in phylogenetic analyses continues to be a contentious topic. MacLeod (2002) discussed this general question and suggested that the hesitation expressed by many at the use of morphometric data in phylogenetics can be traced back to the strong historical connections between morphometrics and phenetics, which was formulated as a philosophy of systematics in the 1960s and 1970s (Pimentel 1979; Sneath and Sokal 1973) and later directly contrasted with the aims and methods of cladistics.
There are three main areas in which morphometrics can play a role: (a) in the generation of characters useful for phylogenetic inference (i.e., estimation of trees); (b) in the interpretation of morphological diversification within the context of a phylogenetic hypothesis produced with other data (typically molecular sequence data); and (c) in “ancestral reconstruction”, the inference about morphological states in ancestors. Of these, the first has been the most problematic. Although many systematists argue that there is no inherent reason for disregarding the use of morphometric data in phylogenetics (Guerrero et al. 2003; MacLeod 2002), others have disagreed (Cranston and Humphries 1988; Crowe 1994; Curnoe 2003; Pimentel and Riggins 1987). But even among those who agree, there is little consensus on the kinds of data that are most appropriate and how they are best to be used. Most relevant to geometric morphometrics have been the arguments by Zelditch et al. (1995), Swiderski (1993), Fink and Zelditch (1995) and Zelditch and Fink (1995) that particular morphometric methods are compatible with the concept of taxic homology. These authors claimed that latent variables such as partial or principal warp decompositions are the only morphometric variables applicable in phylogenetic contexts. Others (Bookstein 1994; Felsenstein 2002; Lynch et al. 1996; Naylor 1996; Rohlf 1998) have challenged or cautioned about the use of geometric morphometric variables for phylogenetic analysis. Additional enlightenment may come from application rather than theory. For example, González-José et al. (2008) have recently shown that when continuous, correlated, modularized morphometric characters are treated as such, cladistic analysis (which is conventionally based on discrete, hypothetically independent characters) can successfully resolve phylogenetic relationships among species of Homo.
MacLeod (2001) reviewed the basic principles involved in viewing morphometric variation within the context of phylogenetically structured comparisons. Excellent recent examples of the use of morphometrics to interpret patterns of morphological diversification include Guill et al. (2003), Magniez-Jannin et al. (2004), and Larson (2005). As for ancestral reconstruction, Rohlf (2002) described a method for estimating ancestral states of shape variables using “squared-change parsimony”, and using the inferred states to depicting shape changes between nodes of the tree as deformations and to estimate the image of an ancestor.
7 Conclusion
The geometric morphometric methods that have been developed over the past several decades to extend beyond the limitations of traditional distance-based methods have become transformed into the new standard research protocol. As the technologies of measurement, analysis and display continue to improve, it will be interesting to see how the current methods evolve over the next few decades.
References
Adams DC, Rohlf FJ, Slice DE (2004) Geometric morphometrics: ten years of progress following the ‘revolution’. Ital J Zool 71: 5–16.
Berge C, Penin X (2004) Ontogenetic allometry, heterochrony, and interspecific differences in the skull of African apes, using tridimensional Procrustes analysis. Am J Phys Anthropol 124: 124–138.
Billoud B, Guerrucci MA, Deutsch JS (2000) Cirripede phylogeny using a novel approach: molecular morphometrics. Mol Biol Evol 17: 1435–1445.
Bookstein FL (1978) The Measurement of Biological Shape and Shape Change. Lecture Notes in Biomathematics 24. Springer-Verlag, New York.
Bookstein FL (1989) Principal warps: thin-plate splines and the decomposition of deformations. IEEE Trans Pattern Anal Machine Intell 11: 567–585.
Bookstein FL (1991) Morphometric Tools for Landmark Data: Geometry and Biology. Cambridge University Press, Cambridge.
Bookstein FL (1994) Can biometrical shape be a homologous character? In Hall BK (ed) Homology: The Hierarchical Basis of Comparative Biology. Academic Press, San Diego, pp 197–227.
Bookstein FL (2004) On a surprising bridge between morphometrics and bioinformatics. Bioinformatics, images, and wavelets, pp. 41–45. Leeds, UK, Leeds Annual Statistical Research Workshop.
Brown JH, West GB, Enquist BJ (2000) Scaling in biology: patterns and processes, causes and consequences. In Brown JH, West GB (eds) Scaling in Biology. Oxford University Press, New York, pp 1–24.
Cranston PS, Humphries CJ (1988) Cladistics and computers: a chironomid conundrum? Cladistics 4: 72–92.
Crowe TM (1994) Morphometrics, phylogenetic models and cladistics: means to an end or much to do about nothing? Cladistics 10: 77–84.
Curnoe D (2003) Problems with the use of cladistic analysis in palaeoanthropology. HOMO 53: 225–234.
Felsenstein J (2002) Quantitative characters, phylogenies, and morphometrics. In MacLeod N, Forey PL (eds) Morphology, Shape and Phylogeny. Taylor and Francis, London, pp 27–44.
Fink WL, Zelditch ML (1995) Phylogenetic analysis of ontogenetic shape transformations: a reassessment of the piranha genus Pygocentrus (Teleostei). Syst Biol 44: 343–360.
Gilbert SF (2003) The morphogenesis of evolutionary developmental biology. Int J Dev Biol 47: 467–477.
González-José R, Escapa I, Neves WA, Cúneo R, Pucciarelli HM (2008) Cladistic analysis of continuous modularized traits provides phylogenetic signals in Homo evolution. Nature 453: 775–778.
Guerrero JA, De Luna E, Sánchez-Hernández C (2003) Morphometrics in the quantification of character state identity for the assessment of primary homology: an analysis of character variation of the genus Artibeus (Chiroptera: Phyllostomidae). Biol J Linn Soc 80: 45–55.
Guill JM, Heins DC, Hood CS (2003) The effect of phylogeny on interspecific body shape variation in darters (Pisces: Percidae). Syst Biol 52: 488–500.
Gunz P, Mitteroecker P, Bookstein FL (2005) Semilandmarks in three dimensions. In Slice DE (ed) Modern Morphometrics in Physical Anthropology. Plenum Publishers, New York, pp 73–98.
Gunz P, Mitteroecker P, Bookstein FL, Weber GW (2002) Approaches to missing data in anthropology. Collegium Antropologicum 26: 78–79.
Hammer Ø (2004) Allometric field decomposition – an attempt at morphogenetic morphometrics. In Elewa AMT (ed) Morphometrics: Applications in Biology and Paleontology. Springer, Berlin, pp 55–65.
Huxley JS (1932) Problems of Relative Growth. Methuen, London.
Kendall DG (1977) The diffusion of shape. Adv Appl Prob 9: 428–430.
Klingenberg CP (2002) Morphometrics and the role of the phenotype in studies of the evolution of developmental mechanisms. Gene 287: 3–10.
Klingenberg CP (2003) Quantitative genetics of geometric shape: heritability and the pitfalls of the univariate approach. Evolution 57: 191–195.
Klingenberg CP, Leamy LJ, Routman EJ, Cheverud JM (2001) Genetic architecture of mandible shape in mice: effects of quantitative trait loci analyzed by geometric morphometrics. Genetics 157: 785–802.
Larson PM (2005) Ontogeny, phylogeny, and morphology in anuran larvae: morphometric analysis of cranial development and evolution in Rana tadpoles (Anura: Ranidae). J Morphol 264: 34–52.
Leamy LJ, Klingenberg CP, Sherratt E, Wolf JB, Cheverud JM (2008) A search for quantitative trait loci exhibiting imprinting effects on mouse mandible size and shape. Heredity 101: 518–526.
Lockwood CA, Lynch JM, Kimbel WH (2002) Quantifying temporal bone morphology of great apes and humans: an approach using geometric morphometrics. J Anat 201: 447–464.
Lynch JM, Wood CG, Luboga SA (1996) Geometric morphometrics in primatology: craniofacial variation in Homo sapiens and Pan troglodytes. Folia Primatol 67: 15–39.
MacLeod N (2001) The role of phylogeny in quantitative paleobiological data analysis. Paleobiology 27: 226–249.
MacLeod N (2002) Phylogenetic signals in morphometric data. In MacLeod N, Forey PL (eds) Morphology, Shape and Phylogeny. Taylor and Francis, London, pp 100–138.
Magniez-Jannin F, David B, Dommergues JL, Zhi-Hui S, Okada TS, Osawa S (2004) Analysing disparity by applying combined morphological and molecular approaches to French and Japanese carabid beetles. Biol J Linn Soc 71: 343–358.
Mitteroecker P, Gunz P (2002) Semilandmarks on curves and surfaces in three dimensions. Am J Phys Anthropol 34 (Suppl): 114–115.
Mitteroecker P, Gunz P, Bernhard M, Schaefer K, Bookstein FL (2004) Comparison of cranial ontogenetic trajectories among great apes and humans. J Hum Evol 46: 679–697.
Naylor GJP (1996) Can partial warp scores be used as cladistic characters? In Marcus LF, Corti M, Loy A, Naylor GJP, Slice DE (eds) Advances in Morphometrics. Plenum Press, New York, pp 519–530.
Nicholson E, Harvati K (2006) Quantitative analysis of human mandibular shape using three-dimensional geometric morphometrics. Am J Phys Anthropol 131: 368–383.
O’Higgins P, Jones N (1998) Facial growth in Cercocebus torquatus: an application of three-dimensional geometric morphometric techniques to the study of morphological variation. J Anat 193: 251–272.
Pimentel RA (1979) Morphometrics: the Multivariate Analysis of Biological Data. Kendall-Hunt, Dubuque.
Pimentel RA, Riggins R (1987) The nature of cladistic data. Cladistics 3: 201–209.
Reig S (1998) 3D digitizing precision and sources of error in the geometric analysis of weasel skulls. Acta Zoologica Academiae Scientiarum Hungaricae 44: 61–72.
Richtsmeier JT, Cheverud JM, Lele SR (1992) Advances in anthropological morphometrics. Annu Rev Anthropol 21: 283–305.
Rogen P, Bohr H (2003) A new family of global protein shape descriptors. Math Biosci 182: 167–181.
Rohlf FJ (1990) Rotational fit (Procrustes) methods. In Rohlf FJ, Bookstein FL (eds) Proceedings of the Michigan Morphometrics Workshop. University of Michigan Museum of Zoology, Ann Arbor, pp 227–236.
Rohlf FJ (1993) Relative warp analysis and an example of its application to mosquito wings. In Marcus LF, Bello E, Garcia-Valdecasas A (eds) Contributions to Morphometrics. Museo Nacional de Ciencias Naturales, Madrid, Spain, pp 131–159.
Rohlf FJ (1998) On applications of geometric morphometrics to studies of ontogeny and phylogeny. Syst Biol 47: 147–158.
Rohlf FJ (2002) Geometric morphometrics and phylogeny. In MacLeod N, Forey PL (eds) Morphology, Shape and Phylogenetics. Taylor and Francis, London, pp 175–193.
Rohlf FJ, Bookstein FL (2003) Computing the uniform component of shape variation. Syst Biol 52: 66–69.
Rohlf FJ, Slice D (1990) Extensions of the Procrustes method for the optimal superposition of landmarks. Syst Zool 39: 40–59.
Rohr K (2001) Landmark-Based Image Analysis: Using Geometric and Intensity Models. Kluwer, Norwell.
Sneath PHA, Sokal RR (1973) Numerical Taxonomy: the Principles and Practice of Numerical Classification. W.H. Freeman, San Francisco.
Strauss RE (1993) The study of allometry since Huxley. In Huxley JS (ed) Problems of Relative Growth. Johns Hopkins University Press, Baltimore, pp 47–75.
Strauss RE, Atanassov MN (2006) Determining best subsets of specimens and characters in the presence of large amounts of missing data. Biol J Linn Soc 88: 309–328.
Strauss RE, Atanassov MN, Oliveira JA (2003) Evaluation of the principal-component and expectation-maximization methods for estimating missing data in morphometric studies. J Vert Paleontol 23: 284–296.
Swiderski DL (1993) Morphological evolution of the scapula in tree squirrels, chipmunks and ground squirrels (Sciuridae): an analysis using thin-plate splines. Evolution 47: 1854–1873.
Thompson DW (1917) On Growth and Form (Reprinted From 1942 Edition). Dover, New York.
Yaroch LA (1996) Shape analysis using the thin-plate spline: neanderthal cranial shape as an example. Yearb Phys Anthropol 39: 43–89.
Zelditch ML, Fink WL (1995) Allometry and developmental integration of body growth in a piranha, Pygocentrus nattereri (Teleostei: Ostariophysi). J Morphol 223: 341–355.
Zelditch ML, Fink WL, Swiderski DL (1995) Morphometrics, homology, and phylogenetics: quantified characters as synapomorphies. Syst Biol 44: 179–189.
Zelditch ML, Lundrigan BL, Sheets HD, Garland T, Jr. (2003) Do precocial mammals develop at a faster rate? A comparison of rates of skull development in Sigmodon fulviventer and Mus musculus domesticus. J Evol Biol 16: 708–720.
Zelditch ML, Swiderski DL, Sheets HD, Fink WL (2004) Geometric Morphometrics for Biologists: A Primer. Academic Press, New York.
Acknowledgments
I particularly thank three colleagues for their stimulating discussions and collaborations: Eric Dyreson for work on 3D thin-plate splines and ancestral reconstructions, Momchil Atanassov for work on missing-data issues, and Raquel Marchán-R for work on allometry in the context of geometric morphometrics.
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Strauss, R.E. (2010). Prospectus: The Future of Morphometrics. In: Elewa, A. (eds) Morphometrics for Nonmorphometricians. Lecture Notes in Earth Sciences, vol 124. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-95853-6_16
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