Abstract
To investigate the correlation between light scattering and tissue viability for brains, we performed multiwavelength diffuse reflectance measurement with perfused brains of rats, in which the reduction level of CuA in cytochrome oxidase was used as an indicator of brain tissue viability. Diffuse reflectance intensity at 620 nm, an isosbestic point of the oxidation-reduction state of cytochrome oxidase, was detected as a scattering signal, while relative diffuse reflectance intensity at 800 nm to that at 620 nm was measured to monitor the absorption change due to the reduction of CuA. After starting perfusion, the scattering signal showed a drastic, triphasic change (increase, decrease and increase) in the time range of 220 – 310 s. After this triphasic change, the scattering signal increased slowly until the end of the measurement (∼ 500 s). The reduction of CuA started and proceeded rapidly during the triphasic scattering change (270 – 310 s). Before and after the triphasic change, we found that light scattering highly correlated with the reduction level of CuA; loss of tissue viability was accompanied by increase in light scattering. These results suggest that the detection of triphasic scattering change is useful to predict loss of tissue viability in brains.
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
Keywords
References
Hossmann KA (1994) Viability thresholds and the penumbra of focal ischemia. Ann Neurol 36:557–565
Harrison DK, Delpy DT (1997) Oxygen Transport to Tissue XIX. Plenum Press, New York
Tao L, Masri D, Hrabetova S et al (2002) Light scattering in rat neocortical slices differs during spreading depression and ischemia. Brain Res 952: 290–300.
Muller M, Somjen GG (1999) Intrinsic optical signals in rat hippocampal slices during hypoxia-induced spreading depression-like depolarization. J Neurophysiol 82:1818–1831
Bahar S, Fayuk D, Somjen GG et al (2000) Mitochondrial and intrinsic optical signals imaged during hypoxia and spreading depression in rat hippocampal slices. J Neurophysiol 84:311–324.
Fujii F, Nodasaka Y, Nishimura G et al (2004) Anoxia induces matrix shrinkage accompanied by an increase in light scattering in isolated brain mitochondria. Brain Res 999:29–39.
Matsunaga A, Nomura Y, Kuroda S et al (1998) Energy-dependent redox state of heme a + a3 and copper of cytochrome oxidase in perfused rat brain in situ. Am J Physiol Cell Physiol 275:C1022–1030.
Nomura Y, Matsunaga A, Tamura M et al (1998) Optical characterization of heme a + a3 and copper of cytochrome oxidase in blood-free perfused rat brain. J Neurosci Methods 82:135–144.
Author information
Authors and Affiliations
Corresponding author
Editor information
Rights and permissions
Copyright information
© 2007 International Federation for Medical and Biological Engineering
About this paper
Cite this paper
Kawauchi, S., Sato, S., Ooigawa, H., Nawashiro, H., Shima, K., Kikuchi, M. (2007). Correlation between light scattering and reduction level of cytochrome oxidase in perfused brains of rats. In: Magjarevic, R., Nagel, J.H. (eds) World Congress on Medical Physics and Biomedical Engineering 2006. IFMBE Proceedings, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-36841-0_311
Download citation
DOI: https://doi.org/10.1007/978-3-540-36841-0_311
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-36839-7
Online ISBN: 978-3-540-36841-0
eBook Packages: EngineeringEngineering (R0)