Abstract
The proximal airway epithelium plays a vital role in host defense through the process of mucociliary clearance. This epithelium is constantly being injured by exposure to toxins and microbials from the environment, but it has an efficient repair mechanism originating from its endogenous stem cells. However, with chronic injury, this repair process can go awry and result in several airway diseases such as chronic obstructive pulmonary disease, asthma, cystic fibrosis, and lung cancer. Understanding the proximal airway epithelium, its stem cell subpopulations, its development, its niche, and the mechanisms regulating its repair are all critical for ultimately developing novel therapies for airway diseases.
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Keywords
- Basal cells
- Submucosal gland duct cells
- Repair
- Regeneration
- Mucociliary clearance
- Pseudostratified columnar epithelium
Introduction
The lung is a complex organ in which the airways and blood vessels form a multigenerational dichotomous branching tree-like structure which is structurally and functionally divided into two main regions: the conducting airways and the air exchange units. The conducting airways are further subdivided into the trachea, bronchi, and bronchioles, while the air exchange units of the distal lung consist of alveoli that are lined by Type I and Type II alveolar epithelial cells.
The more than 42 diverse cell types distributed in different regional microenvironments along the branching airways is an important feature of lung complexity: each cell type designed to perform a specific function. The large conducting airways, which are the subject of this chapter, comprise the trachea and bronchi and are lined by a pseudostratified columnar mucociliary epithelium that functions in host defense. Below this epithelial lining lie the submucosal glands (SMG) which produce the majority of the serous and mucus secretions in the airway. Because these proximal airways are constantly exposed to an external environment which is a potential source of injury caused by challenges including temperature changes, airborne particle inhalation and pathogens, the airway epithelium needs an efficient repair mechanism. It is now well established that these epithelial cell lineages are maintained during homeostasis, and repaired after injury, by regulation of the proliferation and differentiation of stem cells. In this chapter, we describe the significant progress made in recent years in understanding the proximal airway epithelium, its stem and progenitor cell subpopulations, their niche and the mechanisms regulating its repair, while reconciling important differences in the organization of regenerative cells and their regional microenvironments in the human and rodent lung.
Specifically, the cartilaginous trachea and bronchi in the human lung are characterized by the presence of SMG throughout their submucosa, unlike the bronchioles which are the more distal branches of airways that lack cartilage and glands. In rodents however, the distinction between bronchi and bronchioles is less evident as SMG are detected mainly around the first tracheal cartilaginous C-shaped ring (C1) with smaller rudimentary glands being visible down to only C4–6. Rodents also have no cartilage in their intrapulmonary airways. There are also major differences in the epithelial cellular lining of human and rodent airways. In humans, the pseudostratified columnar epithelium consists of basal, ciliated, and secretory cells (mainly goblet cells with a few Club cells—previously known as Clara cells) which line the trachea and bronchi. Whereas in rodents, the pseudostratified columnar epithelium consists of basal, ciliated, and secretory cells (mainly Club and serous cells with almost no goblet cells) which line only the trachea. The intrapulmonary airways down to the alveoli are lined by a simple columnar epithelium that consists mainly of Club and ciliated cells with a few neuroendocrine cells [1–3].
Evidence for Both “Dedicated” and “Facultative” Stem Cells in Proximal Airways
Different organs and tissues have stem and progenitor cells with different proliferative and reparative capacities. Tissues like the skin, intestine, and hematopoietic system have a constant turnover and highly efficient repair after injury. On the other hand, tissues like the brain and heart undergo little turnover and repair poorly after injury, while the lung is characterized by very slow but continuous turnover and variable reparative capacity that depends on the severity, anatomical site, repetition, and nature of injury [4, 5].
Traditionally, quiescence was considered to be an essential property of tissue stem cells. This idea was based on the notion that stem cells are few cells that cycle rarely and slowly in order to protect their genome from developing mutations [6]. Accordingly, a stem cell of a tissue should be able to “retain” a mitotic label for prolonged periods. Label-retaining cells (LRCs) were identified by administering BrdU to animals for long periods, followed by a long washout time. Cells that were identified by immunostaining to retain the BrdU label were considered the stem cells of that tissue. Several studies have identified LRCs in several locations in the proximal airways including the tracheal SMG ducts and dispersed foci within the surface epithelium (probably the inter-cartilaginous regions) of the trachea, as well as Club cells associated with the neuroepithelial bodies [7, 8]. Because lung epithelial turnover is so slow, these studies had to inflict an injury on the lung to induce a state of repair in order to identify LRCs. A recent study suggests that, proximal airway epithelial cells turn over every 6 months, which is much longer than the previous estimates [9, 10]. However, it is possible that the LRCs detected after injury represent a different stem cell subpopulation than the stem cell responsible for maintaining the airways during very slow homeostatic turnover. Indeed, recent findings in the skin and intestine have confirmed that LRCs are not the only stem cells in these tissues, demonstrating that quiescence and label retention are not inherent properties of all tissue stem cells [11].
Similar findings from several tissues have led to the classification of tissue stem cells as either “dedicated” or “facultative.” Dedicated stem cells are thought to be few in number, more undifferentiated, and their self-renewal to be slow (quiescent) and capable of differentiation into more committed progenitors or specialized cell types. Facultative stem cells are thought to represent a larger stem cell pool, which exhibit differentiated features when in the quiescent state but have the capacity to proliferate, self-renew, and give rise to differentiated progeny in response to injury (Fig. 6.1) [12].
Different Stem Cell Subtypes Within the Airway Basal Cell Pool
Basal cells in several organs and tissues express Trp-63 (p63), cytokeratins (K)5 and K14 [13]. Airway basal cells were thought to constitutively express K14 [14] like basal cells of other epithelia, but this was later shown not to be the case [15, 16]. Naïve mouse tracheal basal cells express K14 in only 10–20 % of cells [16, 17] while in apparently healthy human bronchi K14 is expressed in only 1–2 % of cells [18]. K14 is also expressed in the basal and myoepithelial cells (MEC) of the SMG of both human and mouse [17, 18], as discussed below. However, after various injuries and/or infection and with chronic inflammation K14 expression is upregulated in many basal cells [17–19].
The functional role of K14 upregulation after injury and in pathological conditions is not clear. A recent study of stratified epithelium showed that loss of K14 expression was associated with reduced cell proliferation and delay in cell cycle progression [20]. As naïve airway basal cells proliferate very slowly, the need to upregulate K14 expression would be understandable in the context of “activation” of an otherwise-dormant basal stem cell to quickly proliferate in response to injury (Fig. 6.1). Whether K5+/K14− and K5+/K14+ represent two different subtypes of basal cell with some degree of plasticity, or whether they are the same cell in a different state of activation is still controversial [16].
Evidence that basal cells are the “facultative” stem cells of the proximal airways and that they maintain and repair the proximal airways after injury has accumulated over many years [21, 22]. The most concrete characterization of their behavior as stem cells comes from lineage tracing studies. Engelhardt et al. performed a thorough study of the ability of the human proximal airway epithelium to reconstitute a denuded tracheal xenograft [23]. Epithelial cells were color-labeled using retroviral vectors so that the resulting clone sizes and differentiation potential could be followed. They observed that approximately 45 % of clones were multipotent, giving rise to basal, intermediate, secretory, and ciliated cells [23]. This finding is consistent with later rigorous lineage tracing experiments using a K5-CreER transgenic mouse model to follow the fate of K5+ tracheal basal cells in vivo during early postnatal growth, adult tracheal homeostasis, and tracheal repair after injury [24]. This study showed that basal cells self-renew and differentiate into both Club and ciliated cells, and that a long observation period of up to 14 weeks did not lead to loss of the label from basal cells indicating that they are the genuine stem cells in the trachea [24]. By contrast, lineage labeling of tracheal CC10+ (Club) cells showed that while labeled tracheal Club cells do proliferate and differentiate into ciliated cells they are gradually replaced over time by unlabeled cells, presumably the progeny of basal cells [25]. These experiments also indicated that while the differentiative potential of Club cells in basal cell-containing airways was more restricted than that of basal cells during homeostasis or after limited injury, they seemed to be able to generate basal cells after severe epithelial injury (albeit with very low efficiency) [25]. This ability of Club cells to “de-differentiate” into basal cells with a greater developmental potential was recently extensively studied by combining lineage tracing of Club cells with cellular ablation of basal cells (Fig. 6.1) [26]. Hegab et al. used the syngeneic heterotopic transplantation of mouse trachea as a model for severe hypoxic/ischemic injury for airway epithelium [27]. Soon after the injury, most cells of the tracheal surface epithelium underwent apoptosis and sloughed, with only a few surviving cells that were mostly K5+/K14− basal cells. Over the following days, these surviving basal cells, proliferated, acquired K14 expression, and started to differentiate gradually into secretory cells followed later by ciliated cells. BrdU labeling confirmed that most of the proliferating cells through the early repair phase were K5+ basal cells while in the later repair and remodeling period; some secretory cells were also BrdU labeled (Fig. 6.1) [27].
Collectively, it seems that during homeostasis and after different forms of injury, many basal cells throughout the proximal airways possess the ability to self-renew and differentiate towards the secretory and ciliated lineages. Club cells, in the presence of basal cells, have limited proliferative capacity but if basal cells were extensively damaged, they may contribute to the repair process by enhanced proliferation and possibly (de-) differentiation.
One interesting finding from basal cell lineage tracing is that basal cells responded to injury induced by SO2 inhalation by differentiating more towards a ciliated than Club cell fate, while during homeostasis the reverse was true [24]. This suggests that the fate of the basal cell progeny is dependent on interactions with its surrounding environment, namely, the niche. However, it may also indicate the presence of two or more subtypes of basal cells: one that is active during homeostasis and tends to differentiate more into Club cells, while the other subtype is activated in response to injury, which in the case of SO2 inhalation injury produces more ciliated cells. It is also possible that a different subtype of basal cell could respond to different types of injury. When Ghosh et al. lineage traced basal cells after inducing a selective injury to Club cells by naphthalene administration, they detected four different types of clones at 40 days post-injury. 50 % of these clones contained only basal cells, 25 % contained basal and secretory cells, and 19 % of clones had basal, secretory, and ciliated cells, while only 6 % contained basal and ciliated cell clones [16].
Indeed, there is accumulating evidence that there is a subset of basal cells, which has more stem cell characteristics than the rest of the basal cells. When basal cells with higher expression of K5 were sorted from a K5-GFP transgenic mouse, they produced more colonies that were also larger in size compared to basal cells with lower K5 expression [28]. Recently, sorting basal cells based on their aldehyde dehydrogenase activity (a commonly used marker of normal tissue and cancer stem cells [29]), enriched for a subset of basal cells that possessed more efficient two-dimensional (2D) [30] and three-dimensional (3D) colony/sphere formation ability (Fig. 6.1) [19, 31]. In the 3D colony/sphere formation assay, unfractionated basal cell spheres consistently formed spheres with a wide range of sizes [17, 24]. Further analysis of these spheres showed that they also had variable wall thicknesses and ratios of basal to differentiated cells (Hegab AE unpublished data).
Collectively, these data suggest the presence of different types of basal cells that have different responses to injury and possess different proliferative and differentiation abilities. Further studies are warranted to detect markers that can identify these different types in order to perform individualized characterization of their behavior both in vivo and in vitro.
Signaling Pathways Governing Basal Cell Proliferation and Differentiation
It is only recently that we have started to understand the factors regulating basal cell self-renewal versus differentiation during homeostasis and in response to injury. All basal cells in different epithelial tissues are typically marked by the transcription factor p63 (TP63, Trp63). P63 and basal cells seem to be essential for proper function of many organs. For example, loss of p63 from mammary gland basal cells leads to dramatic defects in luminal cell proliferation and differentiation, resulting in lactation failure [32], and loss of p63 in basal cells in the prostate results in disturbed ductal integrity and abnormal differentiation of luminal cells [33]. On the other hand, the trachea of P63 null mice contains no basal cells but shows a well-arranged columnar ciliated epithelium [34].
It is now well established that many stem cells can divide symmetrically, especially during organ development or in response to injury, when cell number expansion becomes a priority to establish an epithelial barrier, and this is also true of airway basal stem cells [35]. Asymmetric cell division (where daughter cells resulting from a cell division adopt divergent fates) is considered as a defining characteristic of stem cell differentiation and is associated with a significant drop in symmetric cell division [35]. Notch has been found to play two temporally distinct roles in airway epithelial repair—firstly, in self-renewal of basal cells and secondly in guiding the differentiation of basal cells towards ciliated or secretory lineages. Tsao et al. used a mouse model with conditional disruption of epithelial (but not mesenchymal) Notch. Inhibition of Notch in the developing lung of this model resulted in intrapulmonary airways that were entirely lined by ciliated cells but did not show any effect on the numbers of tracheal basal cells [36].
Notch has also been found to be important in adult airway epithelium homeostasis and during repair after injury. Using an in vivo reporter allele and genetic and pharmacologic gain- and loss-of-function experiments to demonstrate the role of Notch in cell fate determination of the proximal airway epithelium, Rock et al. found that Notch signaling drove basal cell differentiation to the secretory lineage, while the absence of Notch shifted the balance towards producing more ciliated cells [37]. They also speculated that Notch signaling is not required for self-renewal of basal cells [37]. However, further in-depth studies revealed that different combinations of Notch receptor activation and ligand binding resulted in completely different airway epithelial differentiation profiles [38]. It seems that components of the Notch pathway play complicated and tightly regulated roles in directing basal cell destiny. Paul et al. examined Notch pathway components both in vitro and in vivo to determine their role in controlling basal cell proliferation in response to injury [39]. Wild type mice received intratracheal polidocanol in order to slough off the airway surface epithelium and then the repairing airway epithelium was examined for Notch pathway components. Several components were upregulated during the repair process and were weakly expressed under steady-state conditions, suggesting that some Notch pathway components are active during basal cells proliferation.
Paul et al. also showed that dynamic changes in the level of reactive oxygen species (ROS) directly influence the ability of basal cells to self-renew and proliferate and that this is a tightly regulated process. This intriguing finding also suggests that exogenous ROS from air pollutants and cigarette smoke may influence airway epithelial cell proliferation and differentiation by altering the balance between the self-renewal and differentiation of basal stem cells. Thus, homeostasis is achieved by a regulatory loop in which the rise in ROS levels activates NRF2, the master transcription factor regulating the antioxidant response and NRF2, in its turn, has a direct regulating role on the Notch-mediated role in basal cell proliferation [39].
The epidermal growth factor receptor (EGFR) pathway in the lung epithelium regulates several key cellular processes including self-renewal, proliferation, repair, and differentiation. Aberrant EGFR signaling can cause basal cell hyperplasia, goblet cell hyperplasia, and induction of mucus production and/or promote fibrosis and collagen deposition [40]. An activating mutation in EGFR is associated with non-small cell lung cancer (NSCLC) and drugs that specifically target the EGFR pathway are now being used to treat NSCLC [41]. EGFR was found to have a role in controlling basal cell proliferation in homeostasis and during repair [42]. The EGFR inhibitor, AG1478, decreased basal cell proliferation and mucin production in experimental models [42, 43]. The Wnt-β-catenin pathway has also been shown to play a role in basal cell proliferation and differentiation both in vivo and in vitro [44–46].
One additional aspect of basal cell regulation was delineated with the identification of Myb as a key transcription factor that is expressed in post-mitotic epithelial cells that are destined to become multi-ciliated cells. Myb was shown to act downstream of Notch and upstream of Foxj1 and thus to be an essential regulator of basal cell differentiation towards ciliated lineages [47]. Another study demonstrated that Myb not only marked intermediate cells of the airway epithelium but was also critical in controlling their differentiation to both the ciliated and secretory lineages. Inhibiting Myb in airway epithelium in vitro, and Myb gene deletion in vivo, resulted in p63-negative cells which could not proceed to differentiate into ciliated or secretory cell lineages [48].
The Stem Cell Niche of the Airway Epithelium
It is well established that transcriptional regulation of a stem cell’s decision to proliferate or differentiate towards a specific lineage is markedly affected by mutual signaling with various components in the stem cell niche [49]. Tadokoro et al. have recently described a novel interaction between IL-6, a niche component, and Stat3, a transcription factor. They showed that IL-6 originating from cells in the nearby stroma activate basal cell Stat3-dependent signaling resulting in a shift of basal cell differentiation towards a ciliated cell fate. This regulation was found to be both at the level of ciliogenesis-related genes and transcription factors like the Multicilin gene and FoxJ1, as well as by inhibition of the Notch pathway [50].
Culturing human bronchial and mouse tracheal epithelial cells (MTEC) at an air–liquid interface (ALI) has been the standard assay for studying airway epithelium and its niche in vitro for many years [51, 52]. In this assay, a mixture of airway epithelial cells is seeded on a collagen-coated membrane in a transwell. Cells are submerged in medium and allowed to form “cobblestones” for several days before the medium is removed from the upper chamber to generate an ALI. A pseudostratified epithelium containing basal, Club, ciliated, and goblet cells is usually apparent starting after 5 days at the ALI. Various experiments, treatments, and interventions can be applied to the ALI and the effect on cells can be examined at varying time points.
More recently, the sphere/colony formation assay in a 3D culture system has emerged as a useful method to study and characterize various stem cell properties, such as proliferation, differentiation, and niche interactions [53, 54]. In this assay, the size and number of spheres reflects the proliferation capacity of stem and progenitor cells, while the ability to serially propagate spheres with subsequent passages is considered a measure of self-renewal potential of the basal cells. The sphere assay has several advantages for studying stem cells over the ALI culture system. It allows for direct observation of the proliferation and self-renewal potential of basal cells with various treatments or genetic alterations. Mixing of different types of cells or differently labeled cells, allows for identification of the spatial relationship between putative stem cells and putative niche cells and/or their differentiated progeny. There are an increasing number of studies exploiting this sphere/colony formation assay to identify various properties of airway basal cells and niche interactions [17, 19, 24, 31, 39]. However, as with any in vitro assay, the interpretation of findings about the behavior of basal cells from the sphere/colony formation assay needs to be evaluated carefully and further validated in vivo.
Intermediate Cells
Using specific markers, cell types populating the airway pseudostratified epithelium were identified as basal (30 % of cells), ciliated (30 %), secretory (goblet, serous and/or Club cells) (30 %), and rare neuroendocrine cells (1–2 %) [1, 3]. However, about 8–10 % of cells do not stain for any of these known markers and are known as “indeterminate” cells or “intermediate” cells which are thought to be in a state of transition/differentiation [1, 55]. They were also thought of as early short-lived progenitor cells because they were observed to arise from basal cells that survived SO2 injury before eventually differentiating into ciliated or secretory cell lineages. During this transition, they lost their basal cell markers and had not yet acquired ciliated or Club cell markers [37]. A disturbance in basal cell proliferation and differentiation programs, as might be seen in smokers and patients with COPD, characterized by basal cell hyperplasia or metaplasia is associated with an increase in incidence of both basal and intermediate cells [48]. Likewise, there is an increased incidence of intermediate cells and goblet cells in goblet cell hyperplasia [48]. The recent identification of the transcription factor, Myb, as a marker of these intermediate or early progenitor cells in both mouse and human (Fig. 6.1) [48] will likely pave the way for their further characterization by lineage tracing and exploration of their role during homeostasis and potential contribution to diseases of the lungs.
The Submucosal Glands
SMGs are complex branching structures located in the submucosal tissue of the human trachea and bronchi, but only in the most upper part of the trachea in mice. The SMG ducts open into the epithelium lining the surface of the airways. The duct branches into several collecting ducts which collect secretions from multiple mucus and serous tubules that form the glandular acini. Serous and mucus tubules are lined with polyhedral serous and mucus cells. The SMG tubules are surrounded by elongated MEC which express K5 and K14 in addition to α-smooth muscle actin. The SMG ducts are lined with a thin layer of stratified/pseudostratified epithelium. In the proximal part of the ducts, the luminal cells are ciliated. Both ciliated and basal cells of the SMG ducts seem to be in continuity with the ciliated and basal cells on the surface epithelium but basal cells of the SMG ducts differ from surface epithelial basal cells in that they are more flattened and express K14 [17, 18, 27]. Serous cells of the SMGs secret electrolyte-rich watery secretions, lysozyme, neutral mucins, and immunoglobulins. Mucus cells secret acidic mucins. The rate and nature of secretions from the SMGs is regulated by the cholinergic and non-cholinergic nervous systems and is affected by interactions with inflammatory cells, chemokines and allergens [56, 57]. Hypertrophic SMGs with enhanced secretion volume and a change in secretion constituents are important components of several lung diseases such as chronic bronchitis [58], cystic fibrosis [59], and asthma [60].
Stem Cells of the SMG
Much of the research on SMG stem cells has been conducted using animal models. It is therefore important to consider the structural and cellular differences between rodents and human airways and SMG [61], also highlighted in the introduction, when extrapolating these studies to humans. SMGs develop from the so-called primordial glandular placodes (PGPs), which are clusters of basally positioned epithelial progenitor cells within the developing airway epithelium. These PGPs start to invade into the submucosa and branch in response to signaling cues from the surrounding mesenchyme, eventually forming the branched networks of serous and mucus tubules of the SMGs [61].
Interestingly, when labeled human proximal airway epithelium was used to reconstitute a denuded tracheal xenograft, epithelial cells not only reconstituted the denuded tracheal surface but also invaded the submucosa and formed labeled SMGs, though infrequently [23]. Recently, the gradual appearance of age-related, gland-like structures (ARGLS) has also been described [62]. Interestingly, these ARGLS also seemed to arise by budding from the surface epithelium, similar to what is seen during SMG development and in the xenograft model (Fig. 6.1).
Collectively, it seems that some cells within the proximal airway surface epithelium, presumably some of the basal cells, are responsible for SMG embryonic development and they seem to preserve the potential to “bud” into the submucosa in a transplantation model and with aging.
SMG Duct Cells Are a SMG Stem Cell
Being in a relatively protected environment compared to the airway surface epithelium, SMG cells are expected to cycle even more slowly than the surface epithelium. Ciliated cells in the ducts of the SMG have a turnover rate of more than 18 months, much longer than the 6 months suggested for tracheal ciliated cells [10]. As mentioned above, LRCs were identified in SMG ducts after repeated injury to the surface epithelium [7]. In the same study, the ability of mouse SMG cells to contribute to the tracheal epithelial lining was suggested because when an enzyme-denuded trachea was transplanted subcutaneously in a nude mouse and examined after 28 days, the submucosal region showed cystic spaces lined by a “bronchiolized” ciliated epithelium [7]. These results suggest the potential participation of SMG duct stem cells in repairing injured surface epithelium. However, there is currently no injury model or cell ablation technique available that will specifically injure the SMG to help identify the stem cells maintaining and/or repairing the glands themselves.
Hegab et al. performed several studies to characterize the stem cells in the SMG ducts. In one study, they examined the chronological persistence or reappearance of epithelial cells in a tracheal graft after severe acute hypoxic/ischemic injury [27]. At day 1 after injury, all cells in the SMG bed were apoptotic and only a few K5+/K14+ cells in the gland ducts survived. These surviving duct cells were presumed to be the cells responsible for the ensuing repair [27]. Over the following days, these cells proliferated and gradually differentiated into secretory cells and MEC that invaded deeply into the submucosa eventually forming tubules that appeared to be functional. BrdU labeling confirmed that most proliferating cells during the early repair phase were K5+ duct cells while MECs were also seen to be proliferating during the later stages of repair and remodeling (Fig. 6.1) [27]. This study also confirmed the previous findings of Borthwick et al., [7] by demonstrating the ability of SMG cells to reconstitute the tracheal epithelium; enzymatically denuded prior to transplantation. Hegab et al. showed that SMGs could regenerate the denuded surface epithelium but this process was much slower than when surviving basal cells repaired the surface epithelium (Fig. 6.1) [27]. In another study, an alternative strategy was used to enrich SMG cells based on the finding that SMG duct cells, but not tubular cells, express Trop2 [17]. A 2-step enzyme digestion protocol was used to firstly detach the surface epithelium, and then digest SMG and other stromal cells in a second step prior to FACS sorting SMG duct cells based on their differential expression of Trop2 [17]. In human bronchi, a similar 2-step enzyme digestion protocol was used to separate surface epithelium from SMG cells and then sort basal and SMG duct cells on the basis of their differential expression of CD44 and CD166, respectively [19]. In the mouse, the self-renewal and differentiation potential of sorted SMG duct cells was demonstrated using both in vivo ectopic transplantation and in vitro colony/sphere formation assays, as well as lineage tracing of cells expressing K14 during repair after transplantation.
As mentioned above, in the naïve mouse trachea, K14 is expressed in basal cells of SMG ducts and in MECs but K14+ basal cells only represent about 10 % of the tracheal surface epithelial cells. They used K14CrePR1 mice crossed with ROSA26-floxSTOP-YFP mice in order to express YFP only in K14+ cells on administration of RU486. To induce a proliferative state in the SMGs that turn over extremely slowly, the lineage tracing was performed using the hypoxic-ischemic injury model [17]. When the lineage marker was turned on with RU486 after airway injury, YFP expression was found in the SMGs, SMG ducts, and the SE adjacent to and overlying the SMG ducts but not in the surface epithelium of the distal trachea where SMGs are not present. The detection of this pattern of YFP+ cells strongly suggested that K14+ SMG duct cells contributed to repair of the SMG gland and participated in repair of the surface epithelium together with the basal cells that survived the injury (Fig. 6.1) [17]. As was seen with mouse basal cells [31], colony/sphere forming cells within SMG duct cells were enriched in the ALDH-expressing cell population [19].
SMG Myoepithelial Cells
MECs are flat, thin cells that wrap around the glandular acini, such as mammary, salivary, and airway SMG. Their function is to squeeze acini to help push their secretions into the ducts. They express both smooth muscle actin and keratins. In mammary glands, MECs were found to be the main stem cell of the gland [63] while in salivary glands it was found that the duct cells, rather than MECs, possessed stem cell characteristics [64]. On the other hand, both duct and MEC of the sweat glands seemed to harbor cells with stem cell properties [65, 66]. No study to date has examined the stem cell potential of airway SMG MECs even though they are a potentially clinically relevant cell type as their hypertrophy is commonly associated with fatal asthma [67].
Occasional YFP positive acinar cells were seen shortly after activation of the K14/GFP transgene in the K14CrePR1x ROSA26-floxSTOP-YFP mice by administration of RU486 [Hegab AE unpublished data]. These cells were so distal in the SMGs that it is more likely that they descended from their immediate neighboring K14+ MECs rather than from the more proximally positioned K14+ duct cells. During SMG repair from the severe acute hypoxic/ischemic injury described above, MECs were the first differentiated cell type to appear in addition to the surviving duct cells. BrdU labeling was seen in duct and MECs but not in secretory cells (Fig. 6.1) [27 and Hegab AE unpublished data]. More studies are required to characterize SMG MECs both in vitro and in vivo and to identify their possible contribution to various diseases that involve SMGs.
As the K5/K14-expressing SMG duct cells seem to be the anatomical continuum of the K5/K14-expressing MECs at the junction between the SMG ducts and tubules [17, 19, 27], it is possible that SMGs are hierarchically organized as follows: SMG duct cells have the potential to self-renew and differentiate into both MECs and acinar secretory cells, MECs self-renew and differentiate into acinar secretory cells, while acinar secretory cells are terminally differentiated cells. This is more or less similar to the tracheal cell hierarchy of basal, Club, and ciliated cells, respectively (Fig. 6.1).
Conclusion
In conclusion, much progress has been made over the last 5 years in increasing our understanding of the mechanisms of airway basal stem cell proliferation and differentiation to produce the mucociliary airway epithelium. However, there is still much to be learned about the development and repair of the proximal airway epithelium, its niche and the SMGs. It appears that there are multiple transcriptional pathways that are regulating these processes and the timing of signaling in each is critical for the initial proliferation followed by differentiation in normal airway repair. There is a large amount of overlap of these pathways with those that are active in adult stem cells of other epithelia, and there is much to be learned in studies from organs such as the skin, intestines, breast, and prostate and from other model organisms such as Drosophila melanogaster and Chlamydomonas. Many lung diseases arise from poor mucociliary clearance due to abnormal repair, and premalignant lesions of the large airways arise due to excessive self-renewal and a block in differentiation of basal cells. Thus, there is a great clinical need for the development of new therapies to improve mucociliary clearance and promote the normal pseudostratified columnar airway epithelium. Understanding the airway epithelial cell populations, their stem cell subpopulations, their niche, and the mechanisms involved in their repair will ultimately lead to novel therapies to promote repair of the normal airway epithelium, and a functional mucociliary escalator and thereby improve lung health.
References
Mercer RR, Russell ML, Roggli VL, Crapo JD (1994) Cell number and distribution in human and rat airways. Am J Respir Cell Mol Biol 10(6):613–624
Metzger RJ, Klein OD, Martin GR, Krasnow MA (2008) The branching programme of mouse lung development. Nature 453(7196):745–750
Hogan BL, Barkauskas CE, Chapman HA, Epstein JA, Jain R, Hsia CC et al (2014) Repair and regeneration of the respiratory system: complexity, plasticity, and mechanisms of lung stem cell function. Cell Stem Cell 15(2):123–138
Weiss DJ, Bertoncello I, Borok Z, Kim C, Panoskaltsis-Mortari A, Reynolds S et al (2011) Stem cells and cell therapies in lung biology and lung diseases. Proc Am Thorac Soc 8(3):223–272
Grompe M (2012) Tissue stem cells: new tools and functional diversity. Cell Stem Cell 10(6):685–689
Mackenzie IC, Bickenbach JR (1985) Label-retaining keratinocytes and Langerhans cells in mouse epithelia. Cell Tissue Res 242(3):551–556
Borthwick DW, Shahbazian M, Krantz QT, Dorin JR, Randell SH (2001) Evidence for stem-cell niches in the tracheal epithelium. Am J Respir Cell Mol Biol 24(6):662–670
Hong KU, Reynolds SD, Giangreco A, Hurley CM, Stripp BR (2001) Clara cell secretory protein-expressing cells of the airway neuroepithelial body microenvironment include a label-retaining subset and are critical for epithelial renewal after progenitor cell depletion. Am J Respir Cell Mol Biol 24(6):671–681
Bowden DH (1983) Cell turnover in the lung. Am Rev Respir Dis 128(2 Pt 2):S46–S48
Rawlins EL, Hogan BL (2008) Ciliated epithelial cell lifespan in the mouse trachea and lung. Am J Physiol Lung Cell Mol Physiol 295(1):L231–L234
Li L, Clevers H (2010) Coexistence of quiescent and active adult stem cells in mammals. Science 327(5965):542–545
Rawlins EL, Hogan BL (2006) Epithelial stem cells of the lung: privileged few or opportunities for many? Development 133(13):2455–2465
Hutton E, Paladini RD, Yu QC, Yen M, Coulombe PA, Fuchs E (1998) Functional differences between keratins of stratified and simple epithelia. J Cell Biol 143(2):487–499
Hong KU, Reynolds SD, Watkins S, Fuchs E, Stripp BR (2004) Basal cells are a multipotent progenitor capable of renewing the bronchial epithelium. Am J Pathol 164(2):577–588
Cole BB, Smith RW, Jenkins KM, Graham BB, Reynolds PR, Reynolds SD (2010) Tracheal Basal cells: a facultative progenitor cell pool. Am J Pathol 177(1):362–376
Ghosh M, Brechbuhl HM, Smith RW, Li B, Hicks DA, Titchner T et al (2011) Context-dependent differentiation of multipotential keratin 14-expressing tracheal basal cells. Am J Respir Cell Mol Biol 45(2):403–410
Hegab AE, Ha VL, Gilbert JL, Zhang KX, Malkoski SP, Chon AT et al (2011) Novel stem/progenitor cell population from murine tracheal submucosal gland ducts with multipotent regenerative potential. Stem Cells 29(8):1283–1293
Ooi AT, Mah V, Nickerson DW, Gilbert JL, Ha VL, Hegab AE (2010) Presence of a putative tumor-initiating progenitor cell population predicts poor prognosis in smokers with non-small cell lung cancer. Cancer Res 70(16):6639–6648
Hegab AE, Ha VL, Darmawan DO, Gilbert JL, Ooi AT, Attiga YS et al (2012) Isolation and in vitro characterization of basal and submucosal gland duct stem/progenitor cells from human proximal airways. Stem Cells Transl Med 1(10):719–724
Alam H, Sehgal L, Kundu ST, Dalal SN, Vaidya MM (2011) Novel function of keratins 5 and 14 in proliferation and differentiation of stratified epithelial cells. Mol Biol Cell 22(21):4068–4078
Ford JR, Terzaghi-Howe M (1992) Basal cells are the progenitors of primary tracheal epithelial cell cultures. Exp Cell Res 198(1):69–77
Zepeda ML, Chinoy MR, Wilson JM (1995) Characterization of stem cells in human airway capable of reconstituting a fully differentiated bronchial epithelium. Somat Cell Mol Genet 21(1):61–73
Engelhardt JF, Schlossberg H, Yankaskas JR, Dudus L (1995) Progenitor cells of the adult human airway involved in submucosal gland development. Development 121(7):2031–2046
Rock JR, Onaitis MW, Rawlins EL, Lu Y, Clark CP, Xue Y (2009) Basal cells as stem cells of the mouse trachea and human airway epithelium. Proc Natl Acad Sci U S A 106(31):12771–12775
Rawlins EL, Okubo T, Xue Y, Brass DM, Auten RL, Hasegawa H et al (2009) The role of Scgb1a1+ Clara cells in the long-term maintenance and repair of lung airway, but not alveolar, epithelium. Cell Stem Cell 4(6):525–534
Tata PR, Mou H, Pardo-Saganta A, Zhao R, Prabhu M, Law BM et al (2013) Dedifferentiation of committed epithelial cells into stem cells in vivo. Nature 503(7475):218–223
Hegab AE, Nickerson DW, Ha VL, Darmawan DO, Gomperts BN (2012) Repair and regeneration of tracheal surface epithelium and submucosal glands in a mouse model of hypoxic-ischemic injury. Respirology 17(7):1101–1113
Schoch KG, Lori A, Burns KA, Eldred T, Olsen JC, Randell SH (2004) A subset of mouse tracheal epithelial basal cells generates large colonies in vitro. Am J Physiol Lung Cell Mol Physiol 286(4):L631–L642
Muzio G, Maggiora M, Paiuzzi E, Oraldi M, Canuto RA (2012) Aldehyde dehydrogenases and cell proliferation. Free Radic Biol Med 52(4):735–746
Ghosh M, Helm KM, Smith RW, Giordanengo MS, Li B, Shen H et al (2011) A single cell functions as a tissue-specific stem cell and the in vitro niche-forming cell. Am J Respir Cell Mol Biol 45(3):459–469
Hegab AE, Ha VL, Bisht B, Darmawan DO, Ooi AT, Zhang KX (2014) Aldehyde dehydrogenase activity enriches for proximal airway basal stem cells and promotes their proliferation. Stem Cells Dev 23(6):664–675
Forster N, Saladi SV, van Bragt M, Sfondouris ME, Jones FE, Li Z (2014) Basal cell signaling by p63 controls luminal progenitor function and lactation via NRG1. Dev Cell 28(2):147–160
Kurita T, Medina RT, Mills AA, Cunha GR (2004) Role of p63 and basal cells in the prostate. Development 131(20):4955–4964
Daniely Y, Liao G, Dixon D, Linnoila RI, Lori A, Randell SH (2004) Critical role of p63 in the development of a normal esophageal and tracheobronchial epithelium. Am J Physiol Cell Physiol 287(1):C171–C181
Morrison SJ, Kimble J (2006) Asymmetric and symmetric stem-cell divisions in development and cancer. Nature 441(7097):1068–1074
Tsao PN, Vasconcelos M, Izvolsky KI, Qian J, Lu J, Cardoso WV (2009) Notch signaling controls the balance of ciliated and secretory cell fates in developing airways. Development 136(13):2297–2307
Rock JR, Gao X, Xue Y, Randell SH, Kong YY, Hogan BL (2011) Notch-dependent differentiation of adult airway basal stem cells. Cell Stem Cell 8(6):639–648
Morimoto M, Nishinakamura R, Saga Y, Kopan R (2012) Different assemblies of Notch receptors coordinate the distribution of the major bronchial Clara, ciliated and neuroendocrine cells. Development 139(23):4365–4373
Paul MK, Bisht B, Darmawan DO, Chiou R, Ha VL, Wallace WD et al (2014) Dynamic changes in intracellular ROS levels regulate airway basal stem cell homeostasis through Nrf2-dependent Notch signaling. Cell Stem Cell 15(2):199–214
Vallath S, Hynds RE, Succony L, Janes SM, Giangreco A (2014) Targeting EGFR signalling in chronic lung disease: therapeutic challenges and opportunities. Eur Respir J 44(2):513–522
Taron M, Ichinose Y, Rosell R, Mok T, Massuti B, Zamora L (2005) Activating mutations in the tyrosine kinase domain of the epidermal growth factor receptor are associated with improved survival in gefitinib-treated chemorefractory lung adenocarcinomas. Clin Cancer Res 11(16):5878–5885
Brechbuhl HM, Li B, Smith RW, Reynolds SD (2014) Epidermal growth factor receptor activity is necessary for mouse basal cell proliferation. Am J Physiol Lung Cell Mol Physiol 307(10):L800–L810
Hegab AE, Sakamoto T, Nomura A, Ishii Y, Morishima Y, Iizuka T (2007) Niflumic acid and AG-1478 reduce cigarette smoke-induced mucin synthesis: the role of hCLCA1. Chest 131(4):1149–1156
Li C, Li A, Li M, Xing Y, Chen H, Hu L et al (2009) Stabilized beta-catenin in lung epithelial cells changes cell fate and leads to tracheal and bronchial polyposis. Dev Biol 334(1):97–108
Smith MK, Koch PJ, Reynolds SD (2012) Direct and indirect roles for β-catenin in facultative basal progenitor cell differentiation. Am J Physiol Lung Cell Mol Physiol 302(6):L580–L594
Brechbuhl HM, Ghosh M, Smith MK, Smith RW, Li B, Hicks DA (2011) β-catenin dosage is a critical determinant of tracheal basal cell fate determination. Am J Pathol 179(1):367–379
Tan FE, Vladar EK, Ma L, Fuentealba LC, Hoh R, Espinoza FH (2013) Myb promotes centriole amplification and later steps of the multiciliogenesis program. Development 140(20):4277–4286
Pan JH, Adair-Kirk TL, Patel AC, Huang T, Yozamp NS, Xu J et al (2014) Myb permits multilineage airway epithelial cell differentiation. Stem Cells 32(12):3245–3256
Wagers AJ (2012) The stem cell niche in regenerative medicine. Cell Stem Cell 10(4):362–369
Tadokoro T, Wang Y, Barak LS, Bai Y, Randell SH, Hogan BL (2014) IL-6/STAT3 promotes regeneration of airway ciliated cells from basal stem cells. Proc Natl Acad Sci U S A 111(35):E3641–E3649
You Y, Richer EJ, Huang T, Brody SL (2002) Growth and differentiation of mouse tracheal epithelial cells: selection of a proliferative population. Am J Physiol Lung Cell Mol Physiol 283(6):L1315–L1321
Gray TE, Guzman K, Davis CW, Abdullah LH, Nettesheim P (1996) Mucociliary differentiation of serially passaged normal human tracheobronchial epithelial cells. Am J Respir Cell Mol Biol 14(1):104–112
Pastrana E, Silva-Vargas V, Doetsch F (2011) Eyes wide open: a critical review of sphere-formation as an assay for stem cells. Cell Stem Cell 8(5):486–498
Sato T, van Es JH, Snippert HJ, Stange DE, Vries RG, van den Born M et al (2011) Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts. Nature 469(7330):415–418
Rock JR, Randell SH, Hogan BL (2010) Airway basal stem cells: a perspective on their roles in epithelial homeostasis and remodeling. Dis Model Mech 3(9–10):545–556
Marin MG, Culp DJ (1986) Isolation and culture of submucosal gland cells. Clin Chest Med 7(2):239–245
Ballard ST, Spadafora D (2007) Fluid secretion by submucosal glands of the tracheobronchial airways. Respir Physiol Neurobiol 159(3):271–277
Snider GL (1981) Pathogenesis of emphysema and chronic bronchitis. Med Clin North Am 65(3):647–665
Wood RE, Boat TF, Doershuk CF (1976) Cystic fibrosis. Am Rev Respir Dis 113(6):833–878
Fahy JV (2001) Remodeling of the airway epithelium in asthma. Am J Respir Crit Care Med 164(10 Pt 2):S46–S51
Liu X, Driskell RR, Engelhardt JF (2004) Airway glandular development and stem cells. Curr Top Dev Biol 64:33–56
Wansleeben C, Bowie E, Hotten DF, Yu YR, Hogan BL (2014) Age-related changes in the cellular composition and epithelial organization of the mouse trachea. PLoS One 9(3):e93496
Prater MD, Petit V, Alasdair Russell I, Giraddi RR, Shehata M, Menon S et al (2014) Mammary stem cells have myoepithelial cell properties. Nat Cell Biol 16(10):942–950
Pringle S, Van Os R, Coppes RP (2013) Concise review: adult salivary gland stem cells and a potential therapy for xerostomia. Stem Cells 31(4):613–619
Kurata R, Futaki S, Nakano I, Tanemura A, Murota H, Katayama I et al (2014) Isolation and characterization of sweat gland myoepithelial cells from human skin. Cell Struct Funct 39(2):101–112
Lu CP, Polak L, Rocha AS, Pasolli HA, Chen SC, Sharma N et al (2012) Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair. Cell 150(1):136–150
Green FH, Williams DJ, James A, McPhee LJ, Mitchell I, Mauad T (2010) Increased myoepithelial cells of bronchial submucosal glands in fatal asthma. Thorax 65(1):32–38
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Hegab, A.E., Betsuyaku, T., Gomperts, B.N. (2015). Stem and Progenitor Cells of the Trachea and Proximal Airways. In: Bertoncello, I. (eds) Stem Cells in the Lung. Stem Cell Biology and Regenerative Medicine. Springer, Cham. https://doi.org/10.1007/978-3-319-21082-7_6
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