Summary
Polyclonal antibodies were raised by the repeated injection of rabbits with a synthetic peptide corresponding to a highly selective portion (amino-acid residues 243 to 268) of the amino-acid sequence of the rat 5-HT1A receptor. The anti-peptide antiserum allowed the immunoprecipitation of 5-HT1A receptors but not of other 5-HT receptor types and adrenergic receptors solubilized from rat hippocampal membranes. Immuno-autoradiographic labelling of rat brain sections with the anti-peptide antiserum exhibited the same regional distribution as 5-HT1A sites labelled by selective radioligands such as [3H]8-OH-DPAT and [125I] BH-8-MeO-N-PAT. However, regional differences apparently existed between the respective intensity of labelling by the agonist radioligands and the antiserum, which might be explained by variations in the degree of coupling of 5-HT1A receptor binding subunits with G proteins from one brain area to another.
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References
Albert, P. R., Zhou, Q. Y., Van Tol, H. H. M., Bunzow, J. R., and Civelli, O. (1990). Cloning, functional expression and mRNA tissue distribution of the rat 5-hydroxytryptaminel A receptor gene. J. Biol. Chem. 265: 5825–5832.
Bolaíïos, F. J., Schechter, L. E., Miguel, M. C., Emerit, M. B., Rumigny, J. F., Hamon, M., and Gozlan, H. (1990). Common pharmacological and physico-chemical properties of 5-HT, binding sites in the rat cerebral cortex and NG 108–15 clonal cells. Biochem. Pharmacol. 40: 1541–1550.
Bylund, D. B., and Snyder, S. H. (1976). Beta-adrenergic receptor binding in membrane preparations from mammalian brain. Mol. Pharmacol. 12: 568–580.
Cesselin, F., Soubrié, P., Bourgoin, S., Artaud, F., Reisine, T. D., Michelot, R., Glowinski, J., and Hamon, M. (1981). In vivo release of met-enkephalin in the cat brain. Neuroscience, 6: 301–313.
El Mestikawy, S., Riad, M., Laporte, A. M., Vergé, D., Daval, G., Gozlan, H., and Hamon, M. (1990). Production of specific anti-rat 5-HTIA receptor antibodies in rabbits injected with a synthetic peptide. Neurosci. Lett. 118: 189–192.
El Mestikawy, S., Taussig, D., Gozlan, H., Emerit, M. B., Ponchant, M., and Hamon, M. (1989). Chromatographic analyses of the serotonin 5-HTIA receptor solubilized from the rat hippocampus. J. Neurochem. 53: 1555–1566.
Emerit, M. B., El Mestikawy, S., Gozlan, H., Cossery, J. M., Besselievre, R., Marquet, A., and Hamon, M. (1987). Identification of the 5-HT IA receptor binding subunit in rat brain membranes using the photoaffinity probe [3H]8-methoxy-2-[N,n-propyl,N-3-(2-nitro-4-azidophenyl) aminopropyl]aminotetralin. J. Neurochem. 49: 373–380.
Emerit, M. B., El Mestikawy, S., Gozlan, H., Rouot, B., and Hamon, M. (1990). Physical evidence of the coupling of solubilized 5-HTIA binding sites with G regulatory proteins. Biochem. Pharmacol. 39: 7–18.
Emerit, M. B., Gozlan, H., Marquet, A., and Hamon, M. (1986). Irreversible blockade of central 5-HTIA receptor binding sites by the photoaffinity probe 8-methoxy-3’-NAP-aminoPAT. Eur. J. Pharmacol. 127: 67–81.
Gozlan, H., El Mestikawy, S., Pichat, L., Glowinski, J., and Hamon, M. (1983). Identification of presynaptic serotonin autoreceptors using a new ligand: 3H-PAT. Nature (Lond.) 305: 140–142.
Gozlan, H., Ponchant, M., Daval, G., Vergé, D., Ménard, F., Vanhove, A., Beaucourt, J. P., and Hamon, M. (1988). [12511Bolton-Hunter-8-methoxy-2-[N-propyl-N-propylamino] tetralin as a new selective radioligand of 5-HTIA sites in the rat brain.In vitro binding and autoradiographic studies. J. Pharmacol. Exp. Ther. 244: 751–759.
Hamon, M., Emerit, M. B., El Mestikawy, S., Gallissot, M. C., and Gozlan, H. (1990a). Regional differences in the transduction mechanisms of serotonin receptors in the mammalian brain. In: Saxena, P. R., Wallis, D., Wouters, W., and Bevan, P. (eds), “Cardiovascular Pharmacology of 5-hydroxytryptamine: prospective therapeutic applications”. Kluwer: Dordrecht, pp. 41–59.
Hamon, M., Fattaccini, C. M., Adrien, J., Gallissot, M. C., Martin, P., and Gozlan, H. (1988). Alterations of central serotonin and dopamine turnover in rats treated with ipsapirone and other 5-hydroxytryptamine,A agonists with potential anxiolytic properties. J. Pharmacol. Exp. Ther. 246; 745–752.
Hamon, M., Gozlan, H., El Mestikawy, S., Emerit, M. B., Bolanos, F., and Schechter, L. (1990b). The central 5-HTIA receptors: pharmacological, biochemical, functional and regulatory properties. Ann. N.Y. Acad. Sci. 600: 114–131.
Harada, H., Ueda, H., Katada, T., Ui, M., and Satoh, M. (1990). Phosphorylated µ-opioid receptor purified from rat brains lacks functional coupling with Gil, a GTP-binding protein in reconstituted lipid vesicles. Neurosci. Lett. 113: 47–49.
Hartig, P. R. (1989). Molecular biology of 5-HT receptors. Trends Pharmacol. Sci. 10: 64–69.
Hoyer, D., Engel, G., and Kalkman, H. O. (1985). Molecular pharmacology of 5-HT, and 5-HT, recognition sites in rat and pig brain membranes: radioligand binding studies with [3H]5-HT, [3H18-OH-DPAT, (-)[1251] iodocyanopindolol, [3H]mesulergine and [3H]ketanserin. Eur. J. Pharmacol. 118: 13–23.
Innis, R. B., Nestler, E. J., and Aghajanian, G. K. (1988). Evidence for G protein mediation of serotonin-and GABAB- induced hyperpolarization of rat dorsal raphe neurons. Brain Res. 459: 27–36.
Lee, K. Y., Lew, J. Y., Tang, D., Schlesinger, D. H., Deutch, A. Y., and Goldstein, M. (1989). Antibodies to a synthetic peptide corresponding to a ser-40-containing segment of tyrosine hydroxylase: activation and immunohistochemical localization of tyrosine hydroxylase. J. Neurochem. 53: 1238–1244.
Leysen, J. E., Niemegeers, C. J. E., Van Nueten, J. M., and Laduron, P. M. (1982). [3H]ketanserin (R 41468), a selective [3H]ligand for serotonine receptor binding sites. Binding properties, brain distribution and functional role. Mol. Pharmacol. 21: 301–314.
Meller, E., Goldstein, M., and Bohmaker, K. (1990). Receptor reserve for 5-hydroxytryptamine,A-mediated inhibition of serotonin synthesis: possible relationship to anxiolytic properties of 5-hydroxytryptamine,A agonists. Mol. Pharmacol. 37: 231–237.
Morrow, A. L., and Creese, I. (1986). Characterization of a,-adrenergic receptor subtypes in rat brain: a reevaluation of [3H]WB4101 and [3H]prazosin binding. Mol. Pharmacol. 29: 321–330.
Pazos, A., and Palacios, J. M. (1985). Quantitative autoradiographic mapping of serotonin receptors in the rat brain. I. Serotonin-1 receptors. Brain Res. 346: 205–230.
Roth, B. L., Iadarola, M. J., Mehegan, J. P., and Jacobowitz, D. M. (1989). Immunohistochemical distribution of ß-protein kinase C in rat hippocampus determined with an antibody against a synthetic peptide sequence. Brain Res. Bull. 22: 893–897.
Schechter, L. E., Bolanos, F. J., Gozlan, H., Lanfumey, L., Haj-Dahmane, S., Laporte, A. M., Fattaccini, C. M., and Hamon, M. (1990). Alterations of central serotoninergic and dopaminergic neurotransmission in rats chronically treated with ipsapirone - Biochemical and electrophysiological studies. J. Pharmacol. Exp. Ther. 255: 1335–1347.
Séguéla, P., Watkins, K. C., and Descarries, L. (1989). Ultrastructural relationships of serotonin axon terminals in the cerebral cortex of the adult rat. J. Comp. Neurol. 289: 129–142.
Sotelo, C., Cholley, M., El Mestikawy, S., Gozlan, H., and Hamon, M. (1990). Direct immunohistochemical evidence of the existence of 5-HT,A autoreceptors on serotoninergic neurones in the midbrain raphe nuclei. Eur. J. Neurosci. 2: 1144–1154.
Strader, C. D., Sigal, I. S., and Dixon, R. A. F. (1989). Mapping of functional domains of the ß-adrenergic receptor. Am. J. Respir. Cell. Mol. Biol. 1: 81–86.
Vergé, D., Daval, G., Marcinkiewicz, M., Patey, A., El Mestikawy, S., Gozlan, H. and Hamon, M. (1986). Quantitative autoradiography of multiple 5-HT, receptor subtypes in the brain of control or 5,7-dihydroxytryptamine-treated rats. J. Neurosci. 6: 3474–3482.
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© 1991 Birkhäuser Verlag Basel/Switzerland
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Hamon, M., El Mestikawy, S., Riad, M., Gozlan, H., Daval, G., Vergé, D. (1991). Specific Antibodies as New Tools for Studies of Central 5-HT1AReceptors. In: Fozard, J.R., Saxena, P.R. (eds) Serotonin: Molecular Biology, Receptors and Functional Effects. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7259-1_9
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DOI: https://doi.org/10.1007/978-3-0348-7259-1_9
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