Abstract
In this last chapter, we have briefly summarized several topics treated within the main chapters of this book on the Lymnaeidae. A call of attention is placed upon the integrativeness of research towards this group of snails, from classical to the state-of-the-art approaches. While certain areas of the Lymnaeidae study have been thoroughly covered, some have only been moderately or slightly explored and would need comprehensive research. Hence, we expose some challenges lying ahead for scientists and students that may find themselves passionate with the study of such an interesting family of freshwater snails.
Access provided by Autonomous University of Puebla. Download chapter PDF
Similar content being viewed by others
Keywords
17.1 On the Acknowledgment of Lymnaeid Species: From Identification to Phylogeny
“Integrativeness” is a keyword of current research in many fields of life sciences, from taxonomy to ecology and genetics. Sometimes, it is referred to as the “Age of Integration”, when the multidisciplinary and multi-faceted approaches to research are most welcome (Clamp and Lynn 2017). For research, to be “integrative” means to use data of various origins, taken from different sources and obtained following various methods and techniques. The trend towards integrativeness is markedly expressed in biological systematics, which is thought to rescue this discipline from long-lasting disregard and neglect as well as to overcome the so-called taxonomic impediment (Gomes et al. 2015; Cao et al. 2016; Vinarski 2020).
This concluding chapter aims to review the existing challenges in the lymnaeid research and outline the benefits that different aspects of research can gain from the integrative approach.
The explicit application of the integrative taxonomic approach for classification of the pond snails has started relatively recently (Vinarski et al. 2016; Aksenova et al. 2018), however, the most prominent workers in the field always tried to utilize as many independent sources of data as possible, not using, of course, the very term “integrative taxonomy” (Hubendick 1951; Kruglov 2005). In other words, “taxonomy has been integrative for most of its history” (Valdecasas et al. 2008, p. 211).
Until now, only a very limited number of lymnaeid genera and species complexes has been studied on the “integrative” basis. We still lack molecular information on some small genera of the Lymnaeidae, whereas the phylogenetic relationships and taxonomic position of some other genera cannot be satisfactorily resolved because the available information is too scarce. The new classification scheme for the living Lymnaeidae published in this book (Aksenova et al. 2023, this volume) remains not finalized as the position of some taxa is not clear, and a large deal of further work is required to fulfil the task of classification.
Another challenge for the current lymnaeid systematics is the overload of synonyms and nominal species names in this family, which is among the highest among freshwater Mollusca (Lopes-Lima et al. 2021). Hubendick (1951) compiled a list of about 1150 nominal taxa (including nomina nuda) of the species and below-species rank which were established in this family between 1758 and 1950. Today, such a list is remarkably lengthier and approaches 1500 items (Vinarski, unpublished data). Apparently, only a minority of these names correspond to valid species, however, most of the nomenclaturally available names have not been reassessed since their description, and at least part of these can be suitable for the naming of cryptic species and other entities, which molecular taxonomy can reveal. One of the most urgent tasks of the integrative lymnaeid taxonomy is to link the genetically defined clades of the species rank to already existing species names. The use of old available names is much more preferable than introducing new ones, but such an approach requires a qualification of a “classical” taxonomist, who can work with museum collections and has experience with resolving issues related to zoological nomenclature. Unfortunately, it is not an uncommon situation today, when a team of “integrative” researchers does not include a professional taxonomist, which results in the inability to determine the proper taxonomic position of the revealed species-level clades or describe new species when it is needed. Hence, many current studies (not only in the taxonomy of freshwater Mollusca) suffer from evident incompleteness: their authors are unable to place their findings in a proper taxonomic context, i.e. name and describe biological species revealed by sophisticated analysis. The opposite examples are, of course, available (Aksenova et al. 2018; Mahulu et al. 2019; Vinarski et al. 2020, 2022).
The authors of such publications prefer to deal with “putative” species or “candidate species” rather than to undertake their formal description in accordance with the rules of zoological nomenclature (to give them binomial Latin names). Sometimes it is due to lack of information needed for the proper description, but not rarely does it indicate the absence of an experienced taxonomist in the authors’ team. This practice, however, should not be welcomed because the absence of taxonomic formalization of species hampers future progress in both the studies of biological diversity and conservation efforts (Pante et al. 2015; Vinarski 2020).
Thus, the most desirable kind of taxonomic work with lymnaeids is what combines molecular and morphological study with a thorough exploration of existing literature and available museum collections. Needless to say the truly integrative system of the family Lymnaeidae, both at the genus and below-genus levels, when it is reached will constitute a firm basis for biogeographic and ecological studies of the pond snails as well as for conservation purposes. The use of fossil-calibrated molecular trees with estimates of the absolute ages of certain evolutionary events will allow researchers to put the evolution of the family in a broad paleogeographic context (see, for example, Aksenova et al. 2018). The integrative approach is crucial for the studies of host–parasite systems which are formed by the pond snails and various trematode taxa, for example, for a deepened understanding of coevolution of these groups and the patterns of host specificity of helminths parasitizing the Lymnaeidae (Vázquez et al. 2022).
Up to now, virtually all integrative and molecular research on the pond snail systematics was based on either single-gene (like DNA barcoding) or multi-locus approaches, which has some apparent limitations. The mighty possibilities of the use of complete genomes for phylogeny and taxonomy of the family are still weakly used, and the complete mitochondrial genomes are available for only a few pond snail species (Feldmeyer et al. 2010, 2015; Liu et al. 2012; Qin et al. 2019). The time is ripe for extensive usage of the “omics-approach” for the purpose of lymnaeid classification.
The future of the Lymnaeidae taxonomy will probably benefit from the further advancements of the most innovative approaches, such as the use of machine learning and artificial intelligence (see Quenu et al. 2020; Klinkenbuß et al. 2020 for examples of the application of these techniques to systematic malacology). Today, such traditional tasks of classification as species delimitation are increasingly relegated to computers, which even creates a perspective of the predictable “dehumanization” of biological systematics, the extinction of the scientific community of taxonomists (Vinarski 2022). Are they doomed to repeat the fate of mammoths and the recent victims of the Sixth Extinction? Perhaps, the solution is that, after its “integrative” renewal, systematics will become a constituent part of a synthetic science of biodiversity (or metataxonomy), where taxonomic revisions and descriptions of new taxa, based on an integrated approach, are inseparable from reconstructions of phylogenies, historical biogeographical models, and other products of “non-descriptive” science (Vinarski 2020, 2022).
17.2 Lymnaeid Snails in Applied Medical Malacology
From an applied perspective, in this book we have reviewed several aspects of the lymnaeid studies including experimental models, biological invasions, or disease transmission by the lymnaeid snails. The processes leading to the introduction of lymnaeid snails, whether of natural or anthropic nature, are of particular importance and need further research (Lounnas et al. 2017; Schniebs et al. 2017). However, introduction not necessarily means invasion, and some alien lymnaeid species are unable to expand in the new site (López-Soriano and Quiñonero-Salgado 2020). Invasions are critical events bringing perils to local ecosystem health, threatening not only biodiversity but public health as well when the species is of medical importance. Examples exist within the Lymnaeidae in which introduced species end by resulting in main or relevant hosts of several parasites by either boosting an ongoing transmission (parasite spill-back) (Molloy and Anderson 2006; Carolus et al. 2019) or by bringing new pathogens into the introduction site (parasite spill-over) (Schols et al. 2021).
It is a fact that several lymnaeid species are important hosts of relevant parasites of wildlife, some of great zoonotic relevance (see Hurtrez-Boussès et al. 2023; Vázquez et al. 2023). However, we are still far from the complete understanding of the plethora of species interacting with lymnaeids, particularly in the microbial world (e.g. bacteria and protozoa). We have, to some extent, advanced in elucidating several host–parasite interactions of medical and/or veterinary interest, but mainly in helminth parasites. Within the latter, in the case of lymnaeid snails, the liver flukes (Fasciola spp.) have been thoroughly studied because of their relevance to both medical/veterinary health and meat/milk production (Dreyfuss et al. 2006; Rondelaud et al. 2014; Vázquez et al. 2018). However, we still lack several details on transmission dynamics (mainly related to environmental/climatic interaction), species–species (and genotypic–genotypic) compatibility, ecological risks, etc., that would ultimately determine the presence, susceptibility, and transmissibility by a given lymnaeid population. Following this line, if we dig deeper into the biology of lymnaeid–parasite interactions, research should be aimed at disentangling the effects of the environmental gradients over the population and transmission dynamics. How do differential levels of pollution (chemical, organic, etc.), human perturbations of heterogeneous sorts (habitat transformation, water diversion, etc.), climatic settings (tropical, temperate, alpine, etc.) affect lymnaeids? At the individual level, attention should be steered to the host’s microbiota interactions with pathogens, as we know it might play a major role in driving host–parasite interactions (Le Clec'h et al. 2022). Funding is, however, a big issue for this kind of investigation, but by succeeding in this, we will not only add to the general knowledge on lymnaeid biology, but also better tackle disease transmission.
In an attempt to support such research activities in the laboratory, we have devoted two chapters to explicitly applied works related to lymnaeid snails. Laboratory culture of snails is a fundamental activity to undertake much of the research we have discussed in several chapters. However, such a task has proved to be challenging to researchers around the globe. Conditions needed to rear lymnaeids in the laboratory are quite heterogeneous, as we understand that across the phylogeny of Lymnaeidae we stumble upon species with contrasted patterns of ecological requirements. Thus, the study of lymnaeid ecology is fundamental and more studies should be heading this way. We can share some experiences in some model species (e.g. Galba truncatula, Lymnaea stagnalis, Pseudosuccinea columella, etc.) that range from aquatic to amphibious behaviour, but only solid ecological knowledge would enhance our understanding of more precise conditions that would ultimately translate in better results of our experiments. In this sense, performing experimental infections are critical steps for the general understanding of lymnaeid–parasite interactions, and many studies are routinely carried out in this regard (see Dreyfuss et al. 2023). Detailed conditions for lymnaeid infection, particularly with F. hepatica, in order to measure several parasitological variables are presented in this work.
All in all, we are confident this book will serve as a reference for scientists and students related to the study of the Lymnaeidae, but a broader range of readers such as teachers, parasitologists, malacologists, epidemiologists, paleobiologists, and surveillance and vector control authorities would benefit from the works detailed in their chapters.
References
Aksenova OV, Bolotov IN, Gofarov MY et al (2018) Species richness, molecular taxonomy and biogeography of the radicine pond snails (Gastropoda: Lymnaeidae) in the Old World. Sci Rep 8:11199. https://doi.org/10.1038/s41598-018-29451-1
Aksenova MV, Bolotov IN, Kondakov AV, Vinarski MV (2023, this volume) Phylogeny and taxonomy of the family Lymnaeidae. In: Vinarski MV, Vázquez A (eds) The Lymnaeidae. A handbook on their natural history and parasitological significance. Springer, Cham, pp 67–101
Cao X, Liu J, Chen J et al (2016) Rapid dissemination of taxonomic discoveries based on DNA barcoding and morphology. Sci Rep 6:37066. https://doi.org/10.1038/srep37066
Carolus H, Muzarabani KC, Hammoud C et al (2019) A cascade of biological invasions and parasite spillback in man-made Lake Kariba. Sci Total Environ 659:1283–1292. https://doi.org/10.1016/j.scitotenv.2018.12.307
Clamp JC, Lynn DH (2017) Investigating the biodiversity of ciliates in the ‘age of integration’. Eur J Protistol 61:314–322. https://doi.org/10.1016/j.ejop.2017.01.004
Dreyfuss G, Vignoles P, Mekroud A et al (2006) The presence of uninfected Omphiscola glabra in a breeding of infected Galba truncatula enhanced the characteristics of snail infections with Fasciola hepatica. Parasit Res 99:197–199. https://doi.org/10.1007/s00436-006-0147-6
Dreyfuss G, Vignoles P, Rondelaud D et al (2023, this volume) Rearing and maintenance of lymnaeid snails. In: Vinarski MV, Vázquez A (eds) The Lymnaeidae. A handbook on their natural history and parasitological significance. Springer, Cham, pp 395–414
Feldmeyer B, Hoffmeier K, Pfenninger M (2010) The complete mitochondrial genome of Radix balthica (Pulmonata, Basommatophora), obtained by low coverage shot gun next generation sequencing. Mol Phylogenet Evol 57:1329–1333. https://doi.org/10.1016/j.ympev.2010.09.012
Feldmeyer B, Greshake B, Funke E et al (2015) Positive selection in development and growth rate regulation genes involved in species divergence of the genus radix. BMC Evol Biol 15(1):164. https://doi.org/10.1186/s12862-015-0434-x
Gomes LC, Pessali TC, Sales NG et al (2015) Integrative taxonomy detects cryptic and overlooked fish species in a neotropical river basin. Genetica 143:581–588. https://doi.org/10.1007/s10709-015-9856-z
Hubendick B (1951) Recent Lymnaeidae. Their variation, morphology, taxonomy, nomenclature and distribution. Kungliga Svenska Vetenskapsakademiens Handlingar. Fjärde Serien 3(1):1–223
Hurtrez-Boussès, S, Vittecoq, M, Alba A et al (2023, this volume) Overview of interactions between parasitic Trematoda and lymnaeid snails. In: Vinarski MV, Vázquez A (eds) The Lymnaeidae. A handbook on their natural history and parasitological significance. Springer, Cham, pp 331–357
Klinkenbuß D, Metz O, Reichert J et al (2020) Performance of 3D morphological methods in the machine learning assisted classification of closely related fossil bivalve species of the genus Dreissena. Malacologia 63(1):95–105. https://doi.org/10.4002/040.063.0109
Kruglov ND (2005) Lymnaeid snails of Europe and northern Asia. Smolensk State Pedagogical University Press, Smolensk. [in Russian]
Le Clec'h W, Nordmeyer S, Anderson TJC et al (2022) Snails, microbiomes, and schistosomes: a three-way interaction? Trends Parasitol 38:353. https://doi.org/10.1016/j.pt.2022.01.012
Liu GH, Wang SY, Huang WY et al (2012) The complete mitochondrial genome of Galba pervia (Gastropoda: Mollusca), an intermediate host snail of Fasciola spp. PLoS One 7:e42172. https://doi.org/10.1371/journal.pone.0042172
Lopes-Lima M, Riccardi N, Urbanska M et al (2021) Major shortfalls impairing knowledge and conservation of freshwater molluscs. Hydrobiologia 848(11–12):2831–2867. https://doi.org/10.1007/s10750-021-04622-w
López-Soriano J, Quiñonero-Salgado S (2020) Status of the populations of two alien species of Lymnaeidae in the Ebro Delta (NE Iberian Peninsula). Elona, Revista de Malacología Ibérica 2:17–23
Lounnas M, Correa AC, Vázquez AA et al (2017) Self-fertilization, long-distance flash invasion and biogeography shape the population structure of Pseudosuccinea columella at the worldwide scale. Mol Ecol 26:887–903. https://doi.org/10.1111/mec.13984
Mahulu A, Clewing C, Stelbrink B et al (2019) Cryptic intermediate snail host of the liver fluke Fasciola hepatica in Africa. Parasites Vectors 12:573. https://doi.org/10.1186/s13071-019-3825-9
Molloy J, Anderson G (2006) The distribution of Fasciola hepatica in Queensland, Australia, and the potential impact of introduced snail intermediate hosts. Vet Parasitol 137:62–66. https://doi.org/10.1016/j.vetpar.2005.12.024
Pante E, Schoelinck C, Puillandre N (2015) From integrative taxonomy to species description: one step beyond. Syst Biol 64:152–160. https://doi.org/10.1093/sysbio/syu083
Qin DM, Huang XC, Yang LM et al (2019) Complete mitochondrial genome of the radicine pond snail Radix plicatula (Gastropoda: Lymnaeidae). Mitochondr DNA B 4(2):2861–2862. https://doi.org/10.1080/23802359.2019.1661300
Quenu M, Trewick SA, Brescia F et al (2020) Geometric morphometrics and machine learning challenge currently accepted species limits of the land snail Placostylus (Pulmonata: Bothriembryontidae) on the isle of pines, New Caledonia. J Mollus Stud 86:35–41. https://doi.org/10.1093/mollus/eyz031
Rondelaud D, Titi A, Vignoles P et al (2014) Adaptation of Lymnaea fuscus and Radix balthica to Fasciola hepatica through the experimental infection of several successive snail generations. Parasite Vector 7:296. https://doi.org/10.1186/1756-3305-7-296
Schniebs K, Glöer P, Vinarski M et al (2017) A new alien species in Europe: first record of Austropeplea viridis (Quoy & Gaimard, 1833) (Mollusca, Gastropoda, Lymnaeidae) in Spain. J Conchol 42(5):357–370
Schols R, Carolus H, Hammoud C et al (2021) Invasive snails, parasite spillback, and potential parasite spillover drive parasitic diseases of Hippopotamus amphibius in artificial lakes of Zimbabwe. BMC Biol 19(1):160. https://doi.org/10.1186/s12915-021-01093-2
Valdecasas AG, Williams D, Wheeler QD (2008) ‘Integrative taxonomy’ then and now: a response to Dayrat (2005). Biol J Linn Soc 93:211–216. https://doi.org/10.1111/j.1095-8312.2007.00919.x
Vázquez AA, Alda P, Lounnas M et al (2018) Lymnaeid snails hosts of Fasciola hepatica and Fasciola gigantica (Trematoda: Digenea): a worldwide review. CAB Rev 13:1–15. https://doi.org/10.1079/PAVSNNR201813062
Vázquez AA, Alba A, Alda P et al (2023, this volume) Lymnaeid snails and the transmission of fasciolosis: understanding the differential risks from local to global scale. In: Vinarski MV, Vázquez A (eds) The Lymnaeidae. A handbook on their natural history and parasitological significance. Springer, Cham, pp 359–394
Vázquez AA, Alba A, Alda P et al (2022) On the arrival of fasciolosis in the Americas. Trends Parasitol 38(3):195–204. https://doi.org/10.1016/j.pt.2021.12.001
Vinarski MV (2020) Roots of the taxonomic impediment: is the “integrativeness” a remedy? Integr Zool 15:2–15. https://doi.org/10.1111/1749-4877.12393
Vinarski MV (2022) Today’s taxonomy: a way to a new synthesis or an endless deadlock? Biol Bull Rev 12(6):561–569. https://doi.org/10.1134/S2079086422060093
Vinarski MV, Aksenova OV, Bespalaya YV et al (2016) Radix dolgini: the integrative taxonomic approach supports the species status of a Siberian endemic snail (Mollusca, Gastropoda, Lymnaeidae). C R Biol 339(1):24–36. https://doi.org/10.1016/j.crvi.2015.11.002
Vinarski MV, Aksenova OV, Bolotov IN (2020) Taxonomic assessment of genetically-delineated species of radicine snails (Mollusca, Gastropoda, Lymnaeidae). Zoosyst Evol 96(2):577–608. https://doi.org/10.3897/zse.96.52860
Vinarski MV, von Oheimb PV, Aksenova OV et al (2022) Trapped on the roof of the world: taxonomic diversity and evolutionary patterns of Tibetan plateau endemic freshwater snails (Gastropoda: Lymnaeidae: Tibetoradix). Integr Zool 17:825. https://doi.org/10.1111/1749-4877.12600
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Nature Switzerland AG
About this chapter
Cite this chapter
Vázquez, A.A., Vinarski, M.V. (2023). Perspectives: An Integrated Approach on Future Studies of the Lymnaeidae. In: Vinarski, M.V., Vázquez, A.A. (eds) The Lymnaeidae. Zoological Monographs, vol 7. Springer, Cham. https://doi.org/10.1007/978-3-031-30292-3_17
Download citation
DOI: https://doi.org/10.1007/978-3-031-30292-3_17
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-031-30291-6
Online ISBN: 978-3-031-30292-3
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)