Abstract
Gene expression profile (GEP) studies have provided important insights into the histogenesis and molecular pathogenesis of aggressive T-cell lymphomas. Angioimmunoblastic T-cell lymphoma (AITL) corresponds to T-follicular helper (TFH) lymphocytes and presents consistent deregulation of genes involved in angiogenesis. PTCLs/NOS include at least three different subset characterized by specific cellular derivation (T-central memory, T-cytotoxic and TFH), and possibly different outcome. Besides that, notably, all PTCLs/NOS present with constant deregulation of certain molecules, including the PDGFRA, which represents a suitable therapeutic target in this setting. Finally, both ALK+ and ALK− ALCLs have been shown to be distinct from the other PTCLs, possibly constituting separate entities. As far as prognostication of PTCLs is concerned, the IPI (based on age, performance status, LDH, stage, and extranodal involvement) appears to be efficient as prognostic factor in PTCLs, at least in part and especially for certain PTCL subtypes. However, it is not definitely satisfactory for the two commonest PTCLs, the not otherwise specified (NOS) and the angioimmunoblastic types (AITL). Thus, novel scores, possibly based on the biological features of the tumors have been explored. More recently, GEP has been used for the identification of novel molecular prognostic factors. In particular, inactivation of the NFκB pathway, high expression of proliferation-associated genes, and cytotoxic molecular phenotype seemed to be associated to a worse outcome.
Access provided by Autonomous University of Puebla. Download chapter PDF
Similar content being viewed by others
Keywords
- Overall Survival
- Gene Expression Profile
- International Prognostic Index
- Anaplastic Large Cell Lymphoma
- Gene Expression Profile Study
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Background
In 1994, the Revised European–American Lymphoma (REAL) Classification introduced new standards in the lymphoma field [1]. In particular, it stated for the first time that a classification of lymphoid tumors should consist in a list of “real” entities, each defined by the amalgamation of cell morphology, phenotype, molecular genetics, clinical data, and identification of a normal counterpart, if possible [1]. After a validation trial [2], the REAL Classification was adopted by the World Health Organization (WHO) as guideline for lymphoma diagnosis and therapy [3]. On such occasion, its methodology was extended to all tumors of the haematopoietic system [3]. According to patients’ survival without any treatment, non-Hodgkin lymphomas (NHLs) are classified as indolent (survival measurable in years) and aggressive (survival measurable in months).
Peripheral T-cell lymphomas (PTCLs) belong to the aggressive lymphoma group [4]. They represent approximately 12% of all lymphoid neoplasms [4, 5]. Their incidence varies in different countries and races, being higher in HTLV-1 endemic areas (Asia, Caribbean basin, and some parts of the United States) [4, 5]. PTCLs are a heterogeneous group of tumors that can be roughly subdivided into specified and not otherwise specified (NOS) forms [5, 6]. In particular, the latter—corresponding to about 35% of T-cell lymphomas—cannot be further classified on the basis of morphology, phenotype, and conventional molecular studies [4]. Usually, they occur in the fifth–sixth decade of life, without sex predilection [7–10]. Although PTCLs/NOS can present as isolated disease, they more often have a widespread dissemination (stage III–IV) with nodal, skin, liver, spleen, bone-marrow, and peripheral blood involvement [7–10]. B-symptoms are recorded in about 45% of cases at diagnosis. A haemophagocytic syndrome may also be encountered [7–10].
The tumor morphology is highly variable, comprising cells of different size and shape [4]. PTCLs/NOS may contain prominent reactive components, including small lymphocytes, eosinophils, plasma cells, histiocytes, and epithelioid elements [4].
Immunohistochemistry does generally show T-cell associated molecule expression, although the phenotypic profile is aberrant in about 80% of cases [11].
Clonal rearrangements of T-cell receptor encoding genes are generally detected [12]. The karyotype is aberrant in more than 80% of cases and often characterized by complex abnormalities. However, specific alterations have not been identified [13]. Recently, some recurrent lesions have been documented by comparative genomic hybridization and SNPs analysis [14, 15].
On clinical grounds, PTCLs/NOS are among the most aggressive NHLs. In the majority of cases, the response to conventional chemotherapy is indeed frustrating, with relapse free and overall survival (OS) rates at 5 years below 30% [5].
Besides the PTCL/NOS category, histological classification remains anyway a basic prognostic indicator in the PTCL setting [5, 6, 8, 16]. First, nodal and extranodal entities are clinically well distinct, as extranodal tumors, and specially the cutaneous forms, often display a relatively good outcome [5]. In addition, among nodal PTCLs, the distinction between anaplastic large cell lymphoma (ALCL) and other entities as well as the distinction of ALK+ and ALK− cases among ALCLs retain a significant prognostic impact [5, 6, 8, 16]. In fact, ALK+ ALCL, particularly when occurring in children and young adults, have a significantly better clinical outcome if compared with all other forms [5, 6]. Importantly, it was recently suggested to include ALK− ALCL within the PTCLs/NOS basing on the lack of evidences of clear biological differences between them. However, new clinical and molecular findings demonstrated that ALK− ALCL and PTCL/NOS are distinct entities, also presenting with different clinical outcome [6, 17, 18].
In addition to the basic distinction of the different entities, in that last years several attempts have been made in order to further characterize the molecular pathology of PTCLs and identify reliable prognostic indicators to be offered to clinicians. Indeed, novel insights have been provided by gene expression profile (GEP) studies, specially as far as tumor histogenesis, molecular pathogenesis, and possibly new targeted therapies are concerned. On the other hand, novel and more refined prognostic scores have been proposed, which will be also discussed in this chapter.
Gene Expression Profiling of Peripheral T-Cell Pymphoma
The pathobiology of PTCLs has been neglected for a long time. The main reasons for that probably relied on the relative rarity of the disease, as well as the extreme difficulty to culture these neoplastic cells ex vivo. However, in the last few years, a new interest on PTCLs did emerge. Specially, these lymphomas have been then the object of different studies based on the application of high throughput technologies and several reports dealt with the GEP of the different subtypes [17–32] (Table 3.1). In particular, on one hand, some authors focused on specific topics: that is, the GEP of mycosis fungoides, ALK+ and ALK− ALCLs, angioimmunoblastic lymphomas (AITL), γδ-T-cell lymphomas, adult T-cell lymphoma/leukemia (ATLL), and extranodal NK/T lymphoma nasal-type, respectively [19, 25, 26, 28–31]. On the other hand, others analyzed larger collections of PTCLs of the NOS, AITL, and ALCL types [17, 18, 20, 23, 24, 32]. However, some of these studies suffer of limitations that vary from the usage of chips with a restricted number of genes [20, 23, 24] to the lack of a reliable normal counterpart for comparison [20, 24]. Specifically, Martinez-Delgado et al. reported that PTCL/NOS corresponds to a heterogeneous group of tumors, whose GEP is difficult to interpret due to the significant amount of infiltrating reactive cells. According to these authors, the only clinically relevant information provided by GEP pertains the expression level of genes belonging to the NFκB pathway (see below) [20]. Ballester et al. [23] found that the GEP could discriminate among PTCLs of the NOS, AITL, and ALCL types, although the former did not share a single profile. Using a multi-class predictor, the authors separated their cases into three molecular subgroups called U1, U2, and U3. The U1 gene expression signature included genes known to be associated with poor outcome in other tumors, such as CCND2. The U2 subgroup was associated with over-expression of genes involved in T-cell activation, including NFKB1 and BCL2. The third group was mainly defined by the over-expression of genes involved in the IFN/JAK/STAT pathway and comprised most histiocyte-rich tumors. This finding suggests that the signatures recorded by Ballester et al. might be at least in part influenced by reactive components. Nevertheless, at present, it is not defined yet whether the presence of specific reactive components may significantly affect the tumor behavior in the field of PTCL/NOS, as it appeared in the case of some B-cell derived lymphomas (namely, follicular and Hodgkin lymphomas) [33, 34], and possibly AITL (see below) [32].
Subsequently, Piccaluga et al. [17] have published a GEP study based on the analysis of 28 PTCLs/NOS, all corresponding to lymph node biopsies and containing an amount of neoplastic cells that exceeded the 70% value of the whole examined population. The mRNA extracted from these cases was hybridized on the HG U133 2.0 Plus gene chip. The obtained results were compared with those of six AITLs, six ALCLs (two ALK+ and four ALK−), and 20 samples of normal T-lymphocytes, purified from the peripheral blood and tonsil and corresponding to the main T-cell subsets (CD4+, CD8+, resting, and activated). Thus, the study of Piccaluga et al. significantly differs from most previous reports [20, 23, 24] in terms of methodology and selection criteria. In addition, it provides for the first time the rationale for possible targeted therapies in PTCL/NOS by offering clear evidence of their effectiveness ex vivo. In particular, the GEP detected by Piccaluga et al. [17] indicates that PTCLs/NOS are distinct from the other lymphoid malignancies and normal T-lymphocytes, establishing a clear relationship between PTCL/NOS and normal cellular counterparts and providing the basis for a better understanding of their pathogenesis.
More recently, Iqbal and Colleagues analyzed a large series of PTCLs, collected within the International T-cell lymphoma Project (ITCLP) [32]. Importantly, they could build a robust molecular classifier for ATLL, AITL and ALK+ ALCL. On the other hand, PTCLs/NOS were confirmed to have a more heterogeneous profile possibly related to those of the normal counterparts. In addition, importantly, this study provided novel evidences on AITL and PTCL/NOS prognostication (see below). Additional, important, information have been then offered by Piva et al. [18]. In their study, the authors mainly focused on the molecular pathogenesis of ALCLs but also established the relationship between ALCL and PTCL/NOS. Importantly, they showed that ALCLs are molecularly distinct from PTCL/NOS, thus flattening the diffuse, though not biologically based, proposal of including ALK− ALCL within the group of PTCL/NOS. Consistently, in the course of their analysis, Piccaluga et al. [17] already found that all ALCLs tended to cluster together, irrespectively of their ALK positivity or negativity, though in a more limited number of cases. In all, this suggests that—besides the occurrence or not of a translocation involving the ALK gene at 2p23—these tumors share a set of deregulated pathways. On the other hand, it is possible to clearly differentiate ALK+ and ALK− cases basing on GEP, as shown by different authors [18, 25, 32]. To this regard, in particular, it was demonstrated the strong biological relevance of the ALK/STAT3 signaling in characterizing the global molecular profile of ALK + ALCL [18].
Histogenesis of PTCLs
In the REAL and subsequent WHO classifications of lymphomas, the recognition of the non-neoplastic cellular counterpart is regarded as a main factor contributing to the definition of the single disease entity. However, differently from the field of B-NHLs, the vast majority of PTCLs have not yet definitely associated to a normal counterpart, mainly due to the complexity of T-cell compartment, as well as the bizarre morphology and largely aberrant phenotype of the neoplastic elements. Nevertheless, the recent GEP studies provided evidences which may be the basis for a future classification of these tumors. First, robust data were generated supporting the concept that AITL cells correspond to follicular T-helper (FTH) cells [26, 28]. Specifically, De Leval and Colleagues studied 18 AITL cases and by gene set enrichment analysis [35] found that AITL signature is significantly enriched in molecules characteristic of normal TFH, including CXCL13, BCL6, PDCD1, CD40L, and NFATC1 [26]. At the same time, Piccaluga et al., by using a different algorithm, also showed that the GEP of AITL is definitely related to that of TFH lymphocytes [28]. Noteworthy, both the studies proved that such feature is restricted to AITL cases [26, 28], though a small fraction of PTCLs/NOS, more often characterized by clear cell cytology, presence of blastic EBV+ B-cells, and sometimes, follicular architecture [4] also presents with TFH molecular pattern [26]. Importantly, GEP results were validated by the immunohistochemical demonstration of FTH markers, such as CD10, BCL6, CXCL13, PD1, CCR5, SAP, and ICOS, in AITL [26, 28, 36–40], and were largely in keeping with the observations previously made by Rüdiger et al. [41]. Subsequently, the expression of the same molecules was confirmed in follicular PTCL/NOS [42].
As far as PTCLs/NOS are concerned, interestingly, GEP results suggested that these tumors are more closely related to activated rather than resting T-cells [17]. As in normal mature T-lymphocytes, it was possible to identify two main subgroups of PTCL/NOS, with GEPs related to either CD4 or CD8 elements [17]. Notably, this characteristic did not correspond to the immunophenotype with regards to the expression of the single CD4 and CD8 molecules [17], reflecting the aberrancy in tumor phenotypes [11]. Importantly, the existence of these two molecularly distinct subgroups of PTCL/NOS was later confirmed by the ITCLP study [32]. Remarkably, the latter, also suggested that cases with cytotoxic molecular profile may be provided with a worse prognosis (see below). Finally, ALCLs, according to GEP, appeared to be related to either TH17 [32] (Piccaluga, unpublished) or TH1 lymphocytes (Piccaluga, unpublished).
Overall, the recognition of normal counterparts for different PTCLs has both biological and practical relevance. On one hand, in fact, it provides the basis for the recognition of cellular abnormalities and comprehension of the interaction with the microenvironment (i.e., the relationship of TFH-derived neoplastic cells and follicular dendritic cells, mast cells etc. in AITL). On the other, it can be used in clinics for easier differential diagnosis, by applying new cell specific markers (i.e., a panel of FTH-associated markers for the distinction of AITL and PTCL/NOS) [40].
Molecular Pathogenesis of PTCL
Besides histogenetic information, different GEP studies provided relevant insights into the functional alterations of PTCLs. First, a careful comparison of PTCL/NOS with the closest normal cellular counterparts revealed, in fact, the extensive deregulation of genes, which control functions that are typically damaged in malignant cells, such as matrix remodeling, cell adhesion, transcription regulation, proliferation, and apoptosis. In particular, the analysis of Piccaluga et al. [17] might explain the dissemination pattern of PTCL/NOS, with frequent extranodal and bone-marrow involvement and spread to peripheral blood [4], by showing the up-regulation of FN1, LAMB1, COL1A2, COL3A1, COL4A1, COL4A2, and COL12A1, that is, of genes which promote local invasion and metastasis in different types of human cancers [43–45]. In addition, it revealed the deregulation of genes involved in apoptosis (e.g., MOAP1, ING3, GADD45A, and GADD45B) [46–52] and chemo-resistance (such as CYR61 and NNMT) [43–45, 53–64], which may be responsible for the poor response to conventional chemotherapy. Secondly, a couple of GEP studies suggested the possible deregulation of NFκB pathway in a certain number of PTCL/NOS cases [20, 21, 23]. Indeed, it was shown that around 30–40% of cases present with nuclear localization (i.e., activation) of NFκB elements and peculiar GEP [65]. Noteworthy, it was then demonstrated that a fraction of PTCL/NOS presents with REL locus abnormalities, including amplifications and translocations, finally leading to NFκB constitutive activation [15]. Interestingly, on the other hand, down-regulation of BCL10 (an upstream activator of NFκB in human lymphocytes) was reported to occur in PTCL/NOS [17, 66] with consequent NFκB shut off. However, it is still debated whether (1) such cases NFκB negative cases have a worse clinical outcome and (2) whether anti-NFκB approaches can be eventually effective.
Finally, different studies characterized an aberrant tyrosine–kinase (TK) signaling in PTCLs [17, 18, 22, 31]. In particular, Piccaluga et al. showed that PTCLs/NOS constantly express the PDGRA gene and present with consistent phosphorylation (i.e., activation) of the encoded protein [17, 22]. Intriguingly, the same authors also found PDGF ligands to be produced by PTCL/NOS cells, proposing the intriguing hypothesis of an autocrine/paracrine loop activating this signaling [67]. Noteworthy, it was later on showed that also other T/NK-derived tumors present with such phenomenon with relevant therapeutic implications (see below) [31]. Moreover, Piva et al. demonstrated that STAT3 activation induced by ALK is a major contributor to ALK+ ALCL molecular signature [18].
Importantly, immunohistochemistry was largely adopted in order to provide in situ validation of the genomic data by showing correspondence between mRNA and protein expression, as seen, for example, with PDGFRA [17, 22] and BCL10 [ 17, 66]. In addition, by comparison with normal tissues, immunohistochemistry allowed the identification of staining patterns corresponding to the synthesis of ectopic or paraphysiologic products by neoplastic cells. On the other hand, the phenotypic test highlighted the possibility that some of the results obtained by gene expression profiling may depend on non-neoplastic cellular components present in the analyzed sample, as seen for caldesmon [17].
Identifying Novel Targeted Therapies for PTCL
Tyrosine–Kinase Inhibitors
Basing on the evidence of TK deregulation in different subtypes of PTCLs, the application of TK inhibitors (TKI) has been tested ex vivo [17, 31]. In particular, Piccaluga et al. [17] designed experiments aiming to test the sensitivity of PTCL/NOS cells to different TKI, including imatinib mesylate. The results obtained were of interest, with about 50% cytotoxic effect seen at 48 h with a 1 μmol concentration. Notably, imatinib exerted a limited effect on the viability of normal lymphocytes. On the other hand, Huang et al. treated with imatinib T/NK-tumor-derived cell lines, and obtained analog results [31]. Finally, as far as ALCL are concerned, consistently with GEP data, Chiarle et al. clearly showed that targeting ALK+ and its downstream STAT3 is an effective strategy in ALK+ ALCL [68, 69].
Histone–Deacetylase Inhibitors
Interestingly, GEP analyses provided evidence for the silencing of genes, possibly regulated by epigenetic mechanisms such as acetylation (e.g., GADD45A and GADD45B), and suggested to test histone–deacetylase inhibitors (HDACi) against PTCL/NOS primary cells and cell lines [17]. Notably, these compounds induced a dramatic reduction in cell viability, with G0–G1 cell cycle arrest and apoptosis at therapeutic concentrations, suggesting a possible role for this class of drugs in PTCL/NOS therapy. Noteworthy, this idea was also supported by some clinical preliminary observations [70]. Interestingly, the association of HDACi and daunorubicin apparently had a slight additive effect, as already observed in other settings [71]. Notably, the triple combination of TKI, HDACi, and anthracyclines produced a remarkable effect on cell viability: it might represent a promising option for future therapeutic applications.
Anti-angiogenetic Therapy
Increased angiogenesis is a major characteristic of AITL. However, its molecular basis has been unknown for a long time. Recently, a couple of studies documented the up-regulation of the VEGF gene in this tumor [26, 28]. Importantly, immunohistochemistry, extensively applied to a large series of cases on tissue microarrays, demonstrated that VEGF is mainly expressed by the neoplastic elements [28] and not only by the abundant vascular component, as initially proposed [26]. Remarkably, it was further shown that AITL cells do also express a VEGF-receptor±±±, VEGFR2/KDR [28], suggesting the hypothesis of an autocrine/paracrine stimulation also in this setting. In addition, it suggested the possible AITL sensitivity to anti-angiogenetic drugs, such as thalidomide and bevacizumab. Indeed, several reports have then documented their positive activity in AITL cases [72–77]
Monoclonal Antibodies
During the last few years, therapeutic monoclonal antibodies (MoAb) have become a major component of anti-lymphoma approaches. However, as far as PTCLs are concerned, a significant limitation emerged, differently from what seen in B-NHL. In particular, PTCLs were demonstrated to extensively present with the aberrant expression of T-cell-associated molecules [11, 17]. This phenomenon is indeed relevant in the clinical practice. In fact, some antigens against which, MoAb have been designed, such as CD4 [78] and, specially, CD52 [79, 80], are frequently down-regulated in T-cell tumors [11, 17, 81–83]. Based on these findings, different authors agreed that the estimation of CD52 expression may provide a rationale for the selection of patients with higher probability of responding to alemtuzumab, by avoiding the risk of unwanted toxicity [81]. Similar consideration will have to be applied to other antigens/MoAbs available in the future.
Prognostication of Peripheral T-Cell Lymphomas
Several prognostic indicators (summarised in Table 3.2) have been proposed that will be detailedly discussed in the following.
International Prognostic Index
The international prognostic index (IPI) was first introduced in 1993 with the intent of identifying patients with aggressive NHL and different risk of treatment failure, relapse, and death [84]. It is based on clinical parameters, such as tumor stage, presence of extranodal localizations, age, lactate dehydrogenase (LDH) level, and performance status (PS). Notably, it was built up on B-NHL rather than T-NHL cases, and, specially, diffuses large B-cell lymphomas (DLBCL). However, its ability to stratify PTCL patients was reported in the following years. In particular, the International Lymphoma Study Group showed that overall and relapse free survival were significantly different in patients with low (0/1) vs. high (4/5) IPI, in patients with all PTCL type but ALCL (5-year OS, 36 vs. 15%; 5-year failure free survival, FFS, 27 vs. 10%) [2]. On the other hand, subsequent studies showed that IPI was particularly effective for prognostication of both ALK+ and ALK− ALCL [6, 85]. In particular, in ALK+ ALCL, the 5-year OS was 94 ± 5% for the low/low intermediate risk group vs. 41 ± 12% for the high/high intermediate group (p < 0.0001) [85]. Noteworthy, the IPI was more relevant than ALK expression in stratifying patients with ALCL (relative risk, 3.50 vs. 0.29, respectively), though both were significant prognostic factors [85]. In addition, in a large study within the ITCLP, the IPI effectively identified risk groups with different prognoses within both ALK+ and ALK− ALCL, although those with an IPI score of 3 or more fell into the poor-risk category regardless of ALK status [6].
Furthermore, Suzumiya et al. recently reported on a large series of patients with aggressive adult T-cell leukemia/lymphoma (ATLL), providing evidence that IPI, platelet count, and B-symptoms were significant prognostic factors [86]. Interestingly, multivariate analysis indicated that only the IPI was an independent predictor of OS in this series, though the IPI significantly predicted for OS only in the lymphoma type of ATLL (p = 0.04), but not in the acute one (p = 0.24) [86].
Overall, IPI identifies patients at higher risk, being the expression of disease extension on one hand, and patient’s frailty on the other. Indeed, it does offer neither specific biological hints nor potential target for overcoming drug resistance. In addition, it was soon clear that IPI was not as effective in PTCLs/NOS and AITLs (the two commonest PTCL types) as in the original series of DLBCLs, probably reflecting, at least in part, the fact that PTCL therapy is basically derived from B-NHLs and specific trials have been lacking for a long time. In particular, the extensive use of anthracyclines did not appear to provide significant benefits in PTCL patients [5]. Thus, novel scores have been investigated in this setting in the last few years (see below).
Prognostic Index for PTCL/NOS
In 2004, an Italian group (Intergruppo Italiano Linfomi, IIL) proposed a novel prognostic model based on a retrospective multi-centric clinical analysis of 385 patients [87]. Specifically, the new model, named Prognostic Index for PTCL-U (PIT), included bone-marrow involvement, age, PS, and LDH. When these four variables were combined in four groups, the PIT could identify patient subgroups with different outcomes. Noteworthy, the PIT turned out to be slightly more effective than IPI in stratifying PTCL patients (log-rank 66.79 vs. 55.94) and was then proposed as reference tool. In addition a simplified, two-classes PIT appeared to be superior to a simplified two-classes IPI (log-rank 49.36 vs. 30.23) [87]. However, PIT was based on a series lacking systematic histological review and though its value was confirmed within the ITCLP, it did not resulted superior to IPI. In particular, the PIT was also applied to ALK+ and ALK− ALCL and was similarly predictive of FFS and OS in both groups [6]. However, given that the distribution of patients across the risk groups was very similar with the two prognostic models and that bone-marrow involvement is rarely observed in ALCL, the PIT actually seems to mirror the IPI in this setting [6].
In addition, as it does not include tumor-specific biologic factors, cannot be intended for the future application of targeted therapies.
Clinical-Pathologic Prognostic Score (Bologna Score)
Immunohistochemical markers have been largely proposed for prognostication of malignant lymphomas. As far as PTCLs are concerned, in a large collection of Italian cases, Went et al. recently found that high Ki-67 expression, Epstein–Barr virus (EBV) status, and CD15 staining were associated with the worst outcome in PTCL/NOS [11]. Interestingly, EBV has repeatedly been proposed as a negative prognosticator in PTCLs [88], both among Asian and European patients [89, 90]. Specifically, Went et al. found EBV-positivity in 5 and 3% of PTCLs, NOS, and AITL respectively: this value is definitely lower than the one recorded by Dupuis et al. in a French cohort. Such discrepancies might reflect geographic or racial differences. No other immunohistochemical marker alone or in combination was associated with a poor outcome, although patients with tumors expressing CD57 or CD4+/CD8− phenotype showed a tendency for a better outcome, the possible prognostic relevance of the latter having also been proposed by others [91, 92].
Furthermore, based on their collective largely provided with follow-up data and previous experience in the literature [87, 93–98], Went et al. developed a new score integrating both patient- and tumor-specific characteristics (age >60 years, PS, LDH, and Ki-67 marking ≥80%) and identifying three clear-cut groups of patients with different responses to therapy and life-expectancy. Such score seemed to show an improved ability to predict patient-outcome compared to previous indices, including IPI (p < 0.001 vs. 0.1) and PIT (p < 0.001 vs. 0.0043) [11]. In particular, according to the Bologna score, patients were clustered into three groups, which showed significantly different clinical outcome (median OS 37 vs. 23 vs. 6 months, respectively; p < 0.001) [11].
Interestingly, the ability of the Bologna score was recently validated by a Spanish group [66].
Remarkably, all the factors contributing to the scoring system proposed by Went et al. [11] are part of the routine workup, making their integration simple and cost-effective, and incorporate both patient- and tumor-specific characteristics.
Gene Expression Profiling
In the last few years, several studies dealt with the gene expression profiling (GEP) of nodal PTCLs [17, 18, 20–23, 25–32, 81, 99], possibly providing novel insight into PTCL prognostication. First, a few reports suggested that PTCLs/NOS may present with up- or down-regulation of NFκB molecules [20, 21, 23], with possible prognostic relevance [21, 23]. In particular, cases with higher levels of NFκB-related molecules or other evidence of NFκB activation showed a better median OS (25 months, range 0–124 months, vs. 12 months, range 0–19 months; p = 0.032) [21, 23]. This observation was then confirmed by another Spanish group, the 5-year OS being 45% vs. 0%, in NFκB+ and NFκB− cases, respectively (p = 0.04) [66]. However, all these studies included a relatively limited number of cases, by mixing different histotypes [21, 66], or cases with prominent non-neoplastic components [23], which might have influenced, at least in part the results.
In addition, basing on GEP obtained from 35 nodal PTCL cases (23 PTCLs/NOS and 12 AITLs), it was suggested that over-expression of genes involved in a so-called “proliferation signature” was associated significantly with shorter survival of patients [27]. This proliferation signature included genes commonly associated with the cell cycle, such as CCNA, CCNB, TOP2A, and PCNA [27]. Notably, this evidence of high proliferation as a possible adverse prognostic factor was definitely in line with what reported by Went et al. [11] and what observed within the ITCLP (unpublished), highlighting the importance of such parameter.
Finally, our Group, basing on GEP analyses, indicated that PTCLs/NOS can be subclassified according to their histogenesis. In particular, at least two subgroups were described, derived from activated helper and cytotoxic elements, respectively [17]. Importantly, such finding was recently confirmed by Iqbal et al. [32]. Intriguingly, in this report, it was also suggested that the cytotoxic profile may be associated with unfavorable outcome, though this evidence was based on a limited series and warrants further validation. On the other hand, a possible more favorable outcome for PTCL cases with helper phenotype had been also previously suggested by others [11, 91, 92].
Overall, GEP studies provided evidences that molecular features may be useful in defining the prognosis of PTCL patients. However, no complete explanation has been offered as far as the molecular bases of drug resistance are concerned. Notably, our group described for the first time the expression of molecules associated to drug resistance in solid tumors such as CYR61 and NNMT in PTCL/NOS [17]. Furthermore, Rodríguez-Antona et al. recently found that a high expression of cytochrome P450 3A (CYP3A), an enzyme involved in the inactivation of chemotherapy drugs, was associated to poor response to the standard PTCL chemotherapy, suggesting that CYP3A could be useful as a predictor of response [100]. Indeed, the molecular classification of PTCLs and the identification of key events in their molecular pathology will be probably the basis for future prognostication and targeted treatment in this field as in the case of DLBCL [101, 102].
Prognostication of NK/T-Cell Lymphoma, Nasal-Type
Extranodal natural killer/T-cell lymphoma, nasal-type is a distinct entity in the WHO classification of lymphoid tumors, more frequent in Asia and Central–South America than in Western countries [103–108]. Morphologically, tissue invasion, vascular destruction, and necrosis are the most prominent features; EBV is always integrated in the genome of neoplastic cells [107]. Most of the cases derive from natural killer (NK) cells and are characterized by a typical NK phenotype and T-cell receptor genes in germ-line configuration; however, in some instances a cytotoxic T lymphocyte origin was recognized [107]. The nasal cavity and the upper aerodigestive tract (nasal NK/T-cell lymphoma) are the most commonly involved sites, but skin, gastrointestinal tract, lung, testis, and soft-tissues (extra-nasal NK/T-cell lymphoma) can be also affected [103, 104, 107, 109].
The prognosis of extranodal natural killer/T-cell lymphoma is poor, being the worst among the PTCL categories [108]: survival rate is 30–40%, anyway some differences exist between nasal and non-nasal disease as the latter is more aggressive [107, 108, 110], therefore the inclusion of radiotherapy in treatment protocols improved outcome of nasal natural killer/T-cell lymphoma in stage I or II [106–108, 111, 112]. Among nasal forms, adverse prognostic factors are unfavorable IPI, advanced stage disease (stage III or IV), high circulating EBV DNA levels, and detection of EBV in bone-marrow cells by in situ hybridization [107, 108, 111, 113–117]. Some studies suggest that high proportion of large/transformed cells in tumoral population have a negative impact on survival: anyway the significance of cytological features as prognostic indicator is still uncertain [106–109]. The primary extra-nasal cases are highly aggressive with poor response to therapy even in patients with localized disease [107, 108].
Importantly, a new prognostic index, proposed by a Korean group, specifically developed for NK/T-cell tumors and based on four parameters, (“B” symptoms, LDH levels, stage and regional lymph node involvement) demonstrated a better prognostic stratification of NK/T-cell lymphomas as compared with IPI [105].
In addition, recently, another study showed that four factors (non-nasal-type, stage, performance status and numbers of extranodal involvement) were significant prognostic factors in NK/T-cell lymphomas [118]. Using these four variables, a NK prognostic index was successfully constructed, the 4-year OS of patients with zero, one, two and three or four adverse factors being 55, 33, 15, and 6%, respectively [118].
Conclusion
PTCLs have represented for a long time an orphan pathology. This can be explained by their relatively low incidence (that is anyway higher that of a “common” tumor, such as Hodgkin’s lymphoma), the difficulties encountered in their analysis, and their dismal prognosis. During the last few years, however, a great deal of interest has developed shedding new light on the pathobiology of these tumors and leading to the proposal of more effective prognosticators. In particular, though IPI is somehow effective for PTCL prognostication, novel more refined and possibly disease specific scores have been explored, and several models including clinical–pathological and molecular features have been proposed, their validation process being now ongoing. In addition, innovative therapeutic schedules have been recently proposed, based on the application of the newly developed micro-array techniques. The morning of a new era seems quite close that will actually dissipate the shadows which have wrapped PTCLs for several decades.
References
Harris NL, Jaffe ES, Stein H, et al. A revised European–American classification of lymphoid neoplasms: a proposal from the International Lymphoma Study Group. Blood. 1994;84(5):1361–92.
The Non-Hodgkin’s Lymphoma Classification Project. A clinical evaluation of the International Lymphoma Study Group classification of non-Hodgkin’s lymphoma. Blood. 1997;89(11):3909–18.
WHO. Classification of tumors of hematopoietic and lymphoid tissues. IVth ed. Lyon: IARC; 2008.
Pileri S, Ralfkiaer E, Weisenburger D, et al. Peripheral T-cell lymphoma, not otherwise specified. In: Swerdlow S, Campo E, Harris NL, et al., editors. WHO Classification of tumors of hematopoietic and lymphoid tissues. IVth ed. Lyon: IARC; 2008. p. 429.
Vose J, Armitage J, Weisenburger D. International peripheral T-cell and natural killer/T-cell lymphoma study: pathology findings and clinical outcomes. J Clin Oncol. 2008;26(25):4124–30.
Savage KJ, Harris NL, Vose JM, et al. ALK-anaplastic large-cell lymphoma is clinically and immunophenotypically different from both ALK + ALCL and peripheral T-cell lymphoma, not otherwise specified: report from the International Peripheral T-Cell Lymphoma Project. Blood. 2008;111(12):5496–504.
Evens AM, Gartenhaus RB. Treatment of T-cell non-Hodgkin’s lymphoma. Curr Treat Options Oncol. 2004;5(4):289–303.
Lopez-Guillermo A, Cid J, Salar A, et al. Peripheral T-cell lymphomas: initial features, natural history, and prognostic factors in a series of 174 patients diagnosed according to the R.E.A.L. Classification. Ann Oncol. 1998;9(8):849–55.
Gisselbrecht C, Gaulard P, Lepage E, et al. Prognostic significance of T-cell phenotype in aggressive non-Hodgkin’s lymphomas. Groupe d’Etudes des Lymphomes de l’Adulte (GELA). Blood. 1998;92(1):76–82.
Effect of age on the characteristics and clinical behavior of non-Hodgkin’s lymphoma patients. The Non-Hodgkin’s Lymphoma Classification Project. Ann Oncol. 1997;8(10):973–8.
Went P, Agostinelli C, Gallamini A, et al. Marker expression in peripheral T-cell lymphoma: a proposed clinical-pathologic prognostic score. J Clin Oncol. 2006;24(16):2472–9.
Rudiger T, Weisenburger DD, Anderson JR, et al. Peripheral T-cell lymphoma (excluding anaplastic large-cell lymphoma): results from the Non-Hodgkin’s Lymphoma Classification Project. Ann Oncol. 2002;13(1):140–9.
Zettl A, Rudiger T, Konrad MA, et al. Genomic profiling of peripheral T-cell lymphoma, unspecified, and anaplastic large T-cell lymphoma delineates novel recurrent chromosomal alterations. Am J Pathol. 2004;164(5):1837–48.
Oshiro A, Tagawa H, Ohshima K, et al. Identification of subtype-specific genomic alterations in aggressive adult T-cell leukemia/lymphoma. Blood. 2006;107(11):4500–7.
Hartmann S, Gesk S, Scholtysik R, et al. High resolution SNP array genomic profiling of peripheral T cell lymphomas, not otherwise specified, identifies a subgroup with chromosomal aberrations affecting the REL locus. Br J Haematol. 2010;148(3):402–12.
Ascani S, Zinzani PL, Gherlinzoni F, et al. Peripheral T-cell lymphomas. Clinico-pathologic study of 168 cases diagnosed according to the R.E.A.L. Classification. Ann Oncol. 1997;8(6):583–92.
Piccaluga PP, Agostinelli C, Califano A, et al. Gene expression analysis of peripheral T cell lymphoma, unspecified, reveals distinct profiles and new potential therapeutic targets. J Clin Invest. 2007;117(3):823–34.
Piva R, Agnelli L, Pellegrino E, et al. Gene expression profiling uncovers molecular classifiers for the recognition of anaplastic large-cell lymphoma within peripheral T-cell neoplasms. J Clin Oncol. 2010;28(9):1583–90.
Tracey L, Villuendas R, Dotor AM, et al. Mycosis fungoides shows concurrent deregulation of multiple genes involved in the TNF signaling pathway: an expression profile study. Blood. 2003;102(3):1042–50.
Martinez-Delgado B, Melendez B, Cuadros M, et al. Expression profiling of T-cell lymphomas differentiates peripheral and lymphoblastic lymphomas and defines survival related genes. Clin Cancer Res. 2004;10(15):4971–82.
Martinez-Delgado B, Cuadros M, Honrado E, et al. Differential expression of NF-kappaB pathway genes among peripheral T-cell lymphomas. Leukemia. 2005;19(12):2254–63.
Piccaluga PP, Agostinelli C, Zinzani PL, Baccarani M, Dalla Favera R, Pileri SA. Expression of platelet-derived growth factor receptor alpha in peripheral T-cell lymphoma not otherwise specified. Lancet Oncol. 2005;6(6):440.
Ballester B, Ramuz O, Gisselbrecht C, et al. Gene expression profiling identifies molecular subgroups among nodal peripheral T-cell lymphomas. Oncogene. 2006;25(10):1560–70.
Mahadevan D, Spier C, Della Croce K, et al. Transcript profiling in peripheral T-cell lymphoma, not otherwise specified, and diffuse large B-cell lymphoma identifies distinct tumor profile signatures. Mol Cancer Ther. 2005;4(12):1867–79.
Lamant L, de Reynies A, Duplantier MM, et al. Gene-expression profiling of systemic anaplastic large-cell lymphoma reveals differences based on ALK status and two distinct morphologic ALK + subtypes. Blood. 2007;109(5):2156–64.
de Leval L, Rickman DS, Thielen C, et al. The gene expression profile of nodal peripheral T-cell lymphoma demonstrates a molecular link between angioimmunoblastic T-cell lymphoma (AITL) and follicular helper T (TFH) cells. Blood. 2007;109(11):4952–63.
Cuadros M, Dave SS, Jaffe ES, et al. Identification of a proliferation signature related to survival in nodal peripheral T-cell lymphomas. J Clin Oncol. 2007;25(22):3321–9.
Piccaluga PP, Agostinelli C, Califano A, et al. Gene expression analysis of angioimmunoblastic lymphoma indicates derivation from T follicular helper cells and vascular endothelial growth factor deregulation. Cancer Res. 2007;67(22):10703–10.
Miyazaki K, Yamaguchi M, Imai H, et al. Gene expression profiling of peripheral T-cell lymphoma including gammadelta T-cell lymphoma. Blood. 2009;113(5):1071–4.
Pise-Masison CA, Radonovich M, Dohoney K, et al. Gene expression profiling of ATL patients: compilation of disease-related genes and evidence for TCF4 involvement in BIRC5 gene expression and cell viability. Blood. 2009;113(17):4016–26.
Huang Y, de Reynies A, de Leval L, et al. Gene expression profiling identifies emerging oncogenic pathways operating in extranodal NK/T-cell lymphoma, nasal-type. Blood. 2010;115(6):1226–37.
Iqbal J, Weisenburger DD, Greiner TC, et al. Molecular signatures to improve diagnosis in peripheral T-cell lymphoma and prognostication in angioimmunoblastic T-cell lymphoma. Blood. 2010;115(5):1026–36.
Dave SS, Wright G, Tan B, et al. Prediction of survival in follicular lymphoma based on molecular features of tumor-infiltrating immune cells. N Engl J Med. 2004;351(21):2159–69.
Steidl C, Lee T, Shah SP, et al. Tumor-associated macrophages and survival in classic Hodgkin’s lymphoma. N Engl J Med. 2010;362(10):875–85.
Watson A, Mazumder A, Stewart M, Balasubramanian S. Technology for microarray analysis of gene expression. Curr Opin Biotechnol. 1998;9(6):609–14.
Dupuis J, Boye K, Martin N, et al. Expression of CXCL13 by neoplastic cells in angioimmunoblastic T-cell lymphoma (AITL): a new diagnostic marker providing evidence that AITL derives from follicular helper T cells. Am J Surg Pathol. 2006;30(4):490–4.
Grogg KL, Attygale AD, Macon WR, Remstein ED, Kurtin PJ, Dogan A. Expression of CXCL13, a chemokine highly upregulated in germinal center T-helper cells, distinguishes angioimmunoblastic T-cell lymphoma from peripheral T-cell lymphoma, unspecified. Mod Pathol. 2006;19(8):1101–7.
Roncador G, Garcia Verdes-Montenegro JF, Tedoldi S, et al. Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma. Haematologica. 2007;92(8):1059–66.
Marafioti T, Paterson JC, Ballabio E, et al. The inducible T-cell co-stimulator molecule is expressed on subsets of T cells and is a new marker of lymphomas of T follicular helper cell-derivation. Haematologica. 2010;95(3):432–9.
Laurent C, Fazilleau N, Brousset P. A novel subset of T-helper cells: follicular T-helper cells and their markers. Haematologica. 2010;95(3):356–8.
Rudiger T, Geissinger E, Muller-Hermelink HK. ‘Normal counterparts’ of nodal peripheral T-cell lymphoma. Hematol Oncol. 2006;24(4):175–80.
Huang Y, Moreau A, Dupuis J, et al. Peripheral T-cell lymphomas with a follicular growth pattern are derived from follicular helper T cells (TFH) and may show overlapping features with angioimmunoblastic T-cell lymphomas. Am J Surg Pathol. 2009;33(5):682–90.
Tapper J, Kettunen E, El-Rifai W, Seppala M, Andersson LC, Knuutila S. Changes in gene expression during progression of ovarian carcinoma. Cancer Genet Cytogenet. 2001;128(1):1–6.
Sado Y, Kagawa M, Naito I, et al. Organization and expression of basement membrane collagen IV genes and their roles in human disorders. J Biochem (Tokyo). 1998;123(5):767–76.
van den Boom J, Wolter M, Kuick R, et al. Characterization of gene expression profiles associated with glioma progression using oligonucleotide-based microarray analysis and real-time reverse transcription-polymerase chain reaction. Am J Pathol. 2003;163(3):1033–43.
Jin S, Tong T, Fan W, et al. GADD45-induced cell cycle G2-M arrest associates with altered subcellular distribution of cyclin B1 and is independent of p38 kinase activity. Oncogene. 2002;21(57):8696–704.
Papa S, Zazzeroni F, Bubici C, et al. Gadd45 beta mediates the NF-kappa B suppression of JNK signalling by targeting MKK7/JNKK2. Nat Cell Biol. 2004;6(2):146–53.
Chen F, Lu Y, Zhang Z, et al. Opposite effect of NF-kappa B and c-Jun N-terminal kinase on p53-independent GADD45 induction by arsenite. J Biol Chem. 2001;276(14):11414–9.
Hirose T, Sowa Y, Takahashi S, et al. p53-independent induction of Gadd45 by histone deacetylase inhibitor: coordinate regulation by transcription factors Oct-1 and NF-Y. Oncogene. 2003;22(49):7762–73.
Tan KO, Tan KM, Chan SL, et al. MAP-1, a novel proapoptotic protein containing a BH3-like motif that associates with Bax through its Bcl-2 homology domains. J Biol Chem. 2001;276(4):2802–7.
Nagashima M, Shiseki M, Pedeux RM, et al. A novel PHD-finger motif protein, p47ING3, modulates p53-mediated transcription, cell cycle control, and apoptosis. Oncogene. 2003;22(3):343–50.
Gunduz M, Ouchida M, Fukushima K, et al. Allelic loss and reduced expression of the ING3, a candidate tumor suppressor gene at 7q31, in human head and neck cancers. Oncogene. 2002;21(28):4462–70.
Lee MS, Hanspers K, Barker CS, Korn AP, McCune JM. Gene expression profiles during human CD4+ T cell differentiation. Int Immunol. 2004;16(8):1109–24.
Chtanova T, Newton R, Liu SM, et al. Identification of T cell-restricted genes, and signatures for different T cell responses, using a comprehensive collection of microarray datasets. J Immunol. 2005;175(12):7837–47.
Chtanova T, Tangye SG, Newton R, et al. T follicular helper cells express a distinctive transcriptional profile, reflecting their role as non-Th1/Th2 effector cells that provide help for B cells. J Immunol. 2004;173(1):68–78.
Hosack DA, Dennis Jr G, Sherman BT, Lane HC, Lempicki RA. Identifying biological themes within lists of genes with EASE. Genome Biol. 2003;4(10):R70.
Han JS, Macarak E, Rosenbloom J, Chung KC, Chaqour B. Regulation of Cyr61/CCN1 gene expression through RhoA GTPase and p38MAPK signaling pathways. Eur J Biochem. 2003;270(16):3408–21.
Leu SJ, Liu Y, Chen N, Chen CC, Lam SC, Lau LF. Identification of a novel integrin alpha 6 beta 1 binding site in the angiogenic inducer CCN1 (CYR61). J Biol Chem. 2003;278(36):33801–8.
Schober JM, Lau LF, Ugarova TP, Lam SC. Identification of a novel integrin alphaMbeta2 binding site in CCN1 (CYR61), a matricellular protein expressed in healing wounds and atherosclerotic lesions. J Biol Chem. 2003;278(28):25808–15.
Tsai MS, Bogart DF, Castaneda JM, Li P, Lupu R. Cyr61 promotes breast tumorigenesis and cancer progression. Oncogene. 2002;21(53):8178–85.
Tsai MS, Hornby AE, Lakins J, Lupu R. Expression and function of CYR61, an angiogenic factor, in breast cancer cell lines and tumor biopsies. Cancer Res. 2000;60(20):5603–7.
Lin MT, Chang CC, Chen ST, et al. Cyr61 expression confers resistance to apoptosis in breast cancer MCF-7 cells by a mechanism of NF-kappaB-dependent XIAP up-regulation. J Biol Chem. 2004;279(23):24015–23.
Kassem H, Sangar V, Cowan R, Clarke N, Margison GP. A potential role of heat shock proteins and nicotinamide N-methyl transferase in predicting response to radiation in bladder cancer. Int J Cancer. 2002;101(5):454–60.
Xu J, Capezzone M, Xu X, Hershman JM. Activation of nicotinamide N-methyltransferase gene promoter by hepatocyte nuclear factor-1{beta} in human papillary thyroid cancer cells. Mol Endocrinol. 2005;19(2):527–39.
Piccaluga P, Agostinelli C, Righi S, et al. Expression of classical NF-kappa B pathway molecules in peripheral t-cell lymphoma not otherwise specified. In: 10th International Conference on Malignant Lymphoma; 2008 June, 4–6; 2008; Lugano, CH; 2008. p. #231.
Briones J, Moga E, Espinosa I, et al. Bcl-10 protein highly correlates with the expression of phosphorylated p65 NF-kappaB in peripheral T-cell lymphomas and is associated with clinical outcome. Histopathology. 2009;54(4):478–85.
Piccaluga P, Rossi M, De Falco G, et al. PDGFRA activity deregulation in PTCL/NOS is a consequence of miRNA deregulation and autocrine loop sustainment. In: American Association for Cancer Research Annual Meeting; 2009 April 18–22; Denver Philadelphia (PA): AACR; 2009.
Chiarle R, Simmons WJ, Cai H, et al. Stat3 is required for ALK-mediated lymphomagenesis and provides a possible therapeutic target. Nat Med. 2005;11(6):623–9.
Chiarle R, Martinengo C, Mastini C, et al. The anaplastic lymphoma kinase is an effective oncoantigen for lymphoma vaccination. Nat Med. 2008;14(6):676–80.
Duvic M, Talpur R, Ni X, et al. Phase 2 trial of oral vorinostat (suberoylanilide hydroxamic acid, SAHA) for refractory cutaneous T-cell lymphoma (CTCL). Blood. 2007;109(1):31–9.
Sanchez-Gonzalez B, Yang H, Bueso-Ramos C, et al. Antileukemia activity of the combination of an anthracycline with a histone deacetylase inhibitor. Blood. 2006;108(4):1174–82.
Strupp C, Aivado M, Germing U, Gattermann N, Haas R. Angioimmunoblastic lymphadenopathy (AILD) may respond to thalidomide treatment: two case reports. Leuk Lymphoma. 2002;43(1):133–7.
Bruns I, Fox F, Reinecke P, et al. Complete remission in a patient with relapsed angioimmunoblastic T-cell lymphoma following treatment with bevacizumab. Leukemia. 2005;19(11):1993–5.
Dogan A, Ngu LS, Ng SH, Cervi PL. Pathology and clinical features of angioimmunoblastic T-cell lymphoma after successful treatment with thalidomide. Leukemia. 2005;19(5):873–5.
Ramasamy K, Lim Z, Pagliuca A, Salisbury JR, Mufti GJ, Devereux S. Successful treatment of refractory angioimmunoblastic T-cell lymphoma with thalidomide and dexamethasone. Haematologica 2006;91(8 Suppl): ECR44.
Aguiar Bujanda D. Complete response of relapsed angioimmunoblastic T-cell lymphoma following therapy with bevacizumab. Ann Oncol. 2008;19(2):396–7.
Gottardi M, Danesin C, Canal F, et al. Complete remission induced by thalidomide in a case of angioimmunoblastic T-cell lymphoma refractory to autologous stem cell transplantation. Leuk Lymphoma. 2008;49(9):1836–8.
Kim YH, Duvic M, Obitz E, et al. Clinical efficacy of zanolimumab (HuMax-CD4): two phase 2 studies in refractory cutaneous T-cell lymphoma. Blood. 2007;109(11):4655–62.
Enblad G, Hagberg H, Erlanson M, et al. A pilot study of alemtuzumab (anti-CD52 monoclonal antibody) therapy for patients with relapsed or chemotherapy-refractory peripheral T-cell lymphomas. Blood. 2004;103(8):2920–4.
Gallamini A, Zaja F, Patti C, et al. Alemtuzumab (Campath-1 H) and CHOP chemotherapy as first-line treatment of peripheral T-cell lymphoma: results of a GITIL (Gruppo Italiano Terapie Innovative nei Linfomi) prospective multicenter trial. Blood. 2007;110(7):2316–23.
Piccaluga PP, Agostinelli C, Righi S, Zinzani PL, Pileri SA. Expression of CD52 in peripheral T-cell lymphoma. Haematologica. 2007;92(4):566–7.
Rodig SJ, Abramson JS, Pinkus GS, et al. Heterogeneous CD52 expression among hematologic neoplasms: implications for the use of alemtuzumab (CAMPATH-1 H). Clin Cancer Res. 2006;12(23):7174–9.
Chang ST, Lu CL, Chuang SS. CD52 expression in non-mycotic T- and NK/T-cell lymphomas. Leuk Lymphoma. 2007;48(1):117–21.
A predictive model for aggressive non-Hodgkin’s lymphoma. The International Non-Hodgkin’s Lymphoma Prognostic Factors Project. N Engl J Med. 1993;329(14):987–94.
Falini B, Pileri S, Zinzani PL, et al. ALK + lymphoma: clinico-pathological findings and outcome. Blood. 1999;93(8):2697–706.
Suzumiya J, Ohshima K, Tamura K, et al. The International Prognostic Index predicts outcome in aggressive adult T-cell leukemia/lymphoma: analysis of 126 patients from the International Peripheral T-Cell Lymphoma Project. Ann Oncol. 2009;20(4):715–21.
Gallamini A, Stelitano C, Calvi R, et al. Peripheral T-cell lymphoma unspecified (PTCL-U): a new prognostic model from a retrospective multicentric clinical study. Blood. 2004;103(7):2474–9.
Cheng AL, Su IJ, Chen YC, Uen WC, Wang CH. Characteristic clinicopathologic features of Epstein-Barr virus-associated peripheral T-cell lymphoma. Cancer. 1993;72(3):909–16.
Kluin PM, Feller A, Gaulard P, et al. Peripheral T/NK-cell lymphoma: a report of the IXth Workshop of the European Association for Haematopathology. Histopathology. 2001;38(3):250–70.
Dupuis J, Emile JF, Mounier N, et al. Prognostic significance of Epstein-Barr virus in nodal peripheral T-cell lymphoma, unspecified: A Groupe d’Etude des Lymphomes de l’Adulte (GELA) study. Blood. 2006;108(13):4163–9.
Bekkenk MW, Vermeer MH, Jansen PM, et al. Peripheral T-cell lymphomas unspecified presenting in the skin: analysis of prognostic factors in a group of 82 patients. Blood. 2003;102(6):2213–9.
Kojima H, Hasegawa Y, Suzukawa K, et al. Clinicopathological features and prognostic factors of Japanese patients with “peripheral T-cell lymphoma, unspecified” diagnosed according to the WHO classification. Leuk Res. 2004;28(12):1287–92.
Caulet-Maugendre S, Patey M, Granier E, Joundi A, Gentile A, Caulet T. Quantitative analysis of cellular proliferative activity in 35 T-cell non-Hodgkin’s lymphomas. Use of proliferating cell nuclear antigen and Ki-67 (MIB-1) antibodies and nucleolar organizer regions. Anal Quant Cytol Histol. 1996;18(5):337–44.
Miller TP, Grogan TM, Dahlberg S, et al. Prognostic significance of the Ki-67-associated proliferative antigen in aggressive non-Hodgkin’s lymphomas: a prospective Southwest Oncology Group trial. Blood. 1994;83(6):1460–6.
Mochen C, Giardini R, Costa A, Silvestrini R. MIB-1 and S-phase cell fraction predict survival in non-Hodgkin’s lymphomas. Cell Prolif. 1997;30(1):37–47.
Montalban C, Obeso G, Gallego A, Castrillo JM, Bellas C, Rivas C. Peripheral T-cell lymphoma: a clinicopathological study of 41 cases and evaluation of the prognostic significance of the updated Kiel classification. Histopathology. 1993;22(4):303–10.
Sheval EV, Churakova JV, Dudnik OA, Vorobjev IA. Examination of the proliferative activity of tumor cells in human lymphoid neoplasms using a morphometric approach. Cancer. 2004;102(3):174–85.
Tiemann M, Schrader C, Klapper W, et al. Histopathology, cell proliferation indices and clinical outcome in 304 patients with mantle cell lymphoma (MCL): a clinicopathological study from the European MCL Network. Br J Haematol. 2005;131(1):29–38.
Gazzola A, Bertuzzi C, Agostinelli C, Righi S, Pileri SA, Piccaluga PP. Physiological PTEN expression in peripheral T-cell lymphoma not otherwise specified. Haematologica. 2009;94(7):1036–7.
Rodriguez-Antona C, Leskela S, Zajac M, et al. Expression of CYP3A4 as a predictor of response to chemotherapy in peripheral T-cell lymphomas. Blood. 2007;110(9):3345–51.
Agostinelli C, Piccaluga PP, Went P, et al. Peripheral T cell lymphoma, not otherwise specified: the stuff of genes, dreams and therapies. J Clin Pathol. 2008;61(11):1160–7.
Lenz G, Staudt LM. Aggressive lymphomas. N Engl J Med. 2010;362(15):1417–29.
Quintanilla-Martinez L, Franklin JL, Guerrero I, et al. Histological and immunophenotypic profile of nasal NK/T cell lymphomas from Peru: high prevalence of p53 overexpression. Hum Pathol. 1999;30(7):849–55.
Oshimi K, Kawa K, Nakamura S, et al. NK-cell neoplasms in Japan. Hematology. 2005;10(3):237–45 (Amsterdam, Netherlands).
Lee J, Suh C, Park YH, et al. Extranodal natural killer T-cell lymphoma, nasal-type: a prognostic model from a retrospective multicenter study. J Clin Oncol. 2006;24(4):612–8.
Barrionuevo C, Zaharia M, Martinez MT, et al. Extranodal NK/T-cell lymphoma, nasal type: study of clinicopathologic and prognosis factors in a series of 78 cases from Peru. Appl Immunohistochem Mol Morphol. 2007;15(1):38–44.
Chan J, Quintanilla-Martinez L, Ferry J, Peh S-C. Extranodal NK/T-cell lymphoma, nasal-type. In: Swerdlow S, Campo E, Harris NL, et al., editors. WHO Classification of tumors of hematopoietic and lymphoid tissues. IVth ed. Lyon: IARC; 2008. p. 285.
Au WY, Weisenburger DD, Intragumtornchai T, et al. Clinical differences between nasal and extranasal natural killer/T-cell lymphoma: a study of 136 cases from the International Peripheral T-Cell Lymphoma Project. Blood. 2009;113(17):3931–7.
Kwong YL, Chan AC, Liang R, et al. CD56+ NK lymphomas: clinicopathological features and prognosis. Br J Haematol. 1997;97(4):821–9.
Chan JK. Natural killer cell neoplasms. Anat Pathol. 1998;3:77–145.
Cheung MM, Chan JK, Lau WH, Ngan RK, Foo WW. Early stage nasal NK/T-cell lymphoma: clinical outcome, prognostic factors, and the effect of treatment modality. Int J Radiat Oncol Biol Phys. 2002;54(1):182–90.
Kuo TT, Shih LY, Tsang NM. Nasal NK/T cell lymphoma in Taiwan: a clinicopathologic study of 22 cases, with analysis of histologic subtypes, Epstein-Barr virus LMP-1 gene association, and treatment modalities. Int J Surg Pathol. 2004;12(4):375–87.
Au WY, Pang A, Choy C, Chim CS, Kwong YL. Quantification of circulating Epstein-Barr virus (EBV) DNA in the diagnosis and monitoring of natural killer cell and EBV-positive lymphomas in immunocompetent patients. Blood. 2004;104(1):243–9.
Ng SB, Lai KW, Murugaya S, et al. Nasal-type extranodal natural killer/T-cell lymphomas: a clinicopathologic and genotypic study of 42 cases in Singapore. Mod Pathol. 2004;17(9):1097–107.
Chim CS, Ma SY, Au WY, et al. Primary nasal natural killer cell lymphoma: long-term treatment outcome and relationship with the International Prognostic Index. Blood. 2004;103(1):216–21.
Huang WT, Chang KC, Huang GC, et al. Bone marrow that is positive for Epstein-Barr virus encoded RNA-1 by in situ hybridization is related with a poor prognosis in patients with extranodal natural killer/T-cell lymphoma, nasal type. Haematologica. 2005;90(8):1063–9.
Lee J, Suh C, Huh J, et al. Effect of positive bone marrow EBV in situ hybridization in staging and survival of localized extranodal natural killer/T-cell lymphoma, nasal-type. Clin Cancer Res. 2007;13(11):3250–4.
Suzuki R, Suzumiya J, Yamaguchi M, et al. Prognostic factors for mature natural killer (NK) cell neoplasms: aggressive NK cell leukemia and extranodal NK cell lymphoma, nasal type. Ann Oncol. 2010;21(5):1032–40.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this chapter
Cite this chapter
Piccaluga, P.P., Pileri, S.A. (2013). Molecular Profiling and Prognosis in T-Cell Lymphomas. In: Foss, F. (eds) T-Cell Lymphomas. Contemporary Hematology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-170-7_3
Download citation
DOI: https://doi.org/10.1007/978-1-62703-170-7_3
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-169-1
Online ISBN: 978-1-62703-170-7
eBook Packages: MedicineMedicine (R0)