Abstract
Cerebrospinal fluid (CSF), a clear colorless fluid with a viscosity similar to water, is produced at a rate of approx 500 mL p/d. The volume averages 135–150 mL in an adult and approx 40 mL in neonates with a turnover rate of 6 h. Approximately two-thirds of the CSF is secreted by the choroid plexuses, whereas the rest comes from leakage of plasma from the capillary bed found in the central nervous system (CNS) and the metabolism of glucose by cells in the CNS.
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References
Fishman, R. A. (1992) Cerebrospinal Fluid in Diseases of the Nervous System, 2nd ed. W. B. Saunders, Philadelpha, PA.
McFarlin, D. E. and McFarlin, H. F. (1982) Multiple sclerosis. N. Engl. J. Med. 307, 1974–1977.
Tourtellote, W. W., Haerer, A. F., Fleming, J. O., Murthy, K. N., Levy, J., and Brandes, D. W. (1975) Cerebrospinal fluid (CSF) immunoglobulins-G (IgG) of extravascular orgin in normals and patients with multiple sclerosis (MS): clinical correlation. Trans. Am. Neurol. Assoc. 100, 250–252.
Johnson, K. P., Arrigo, S. C., and Nelson, B. J. (1977) Agarose electrophoresis of cerebospinal in multiple sclerosis. Neurology 27, 273–277.
Link, H. and Miller, R. (1971) Immunoglobins in muliple sclerosis and infections of the nervous system. Arch. Neurol. 25, 326–344.
Olsson, J. E. and Pettersson, B. (1976) A comparsion between agar gel electrophoresis and CSF serum quotients of IgG and albumin in neurological diseases. Acta. Neurol. Scand. 53, 308–322.
Epstein, E., Zak, B., Baginski, E. J., and Civin, H. (1976) Interpertation of cerebospinal fluid by gel electrophoresis. Ann. Clin. Lab. Sci. 6, 27–37.
Thompson, E. J. (1977) Laboratory diagnosis of multisclerosis: immunological and biochemical aspects. Br. Med. Bull. 33, 28–33.
Crowley, G., Gould, B., Rees, J., and Firth, G. (1990) Separation and detection of alkaline oligoclonal IgG band in cerbrospinal fluid using immobilized pH gradients. Electrophoresis 16, 1922–1926.
Wiederkehr, F., Ogilivie, A., and Vonderschmitt, D. (1987) Cerbrospinal fluid proteins studied by two-demsional gel electrophoresis and immunoblotting technique. J. Neurochem. 49, 363–372.
Mehta, P. D. (1984) Silver staining of unconcentrated cerbrospinal fluid in agarose gel (Panagel) elecctrophoresis. Clin. Chem. 30, 735–736.
Cowdrey, G., Firth, M., and Firth, G. (1995) Separation of cerebrospinal fluid proteins using capillary electrophoresis: a potential method for the diagnosis of neurological disorders. Electrophoresis 16, 1922–1926.
Cowdrey, G., Firth, M., and Firth, G. (1999) Electrophoresis of cerebrospinal fluid, in Methods in Molecular Medicine, vol. 27: Clinical Applications of Capillary Electrophoresis. Palfrey, S. M., ed., Humana Press, Totowa, NJ.
Sanders, E., Katzmann, J. A., Clark, R., Oda, R. P., Shihabi, Z., and Landers, J. P. (1998) Development of capillary electrophoresis as an alternative to high resolution agarose electrophoresis for the diagnosis of multiple sclerosis. Clin. Chem. Lab. Med. 37, 37–45.
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Petersen, J.R., Mohammad, A.A. (2001). Cerebrospinal Fluid Protein Electrophoresis. In: Petersen, J.R., Mohammad, A.A. (eds) Clinical and Forensic Applications of Capillary Electrophoresis. Pathology and Laboratory Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-120-6_6
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DOI: https://doi.org/10.1007/978-1-59259-120-6_6
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