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Capillary Coatings for Protein Analysis

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Clinical and Forensic Applications of Capillary Electrophoresis

Part of the book series: Pathology and Laboratory Medicine ((PLM))

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Abstract

Protein analysis has become very important to the biotechnology, pharmaceutical, and food industries. Analytical techniques that provide fast, automated analysis, but still give high resolution separation of proteins derived from various sources, such as plasma, blood, and vaccine products, are in high demand. Chromatography and electrophoresis continue to be the preferred means for analyzing proteins. Capillary electrophoresis (CE), a micro-format of electrophoresis, has been successfully used for separating various proteins. A major problem encountered in CE analysis of proteins using a fused silica capillary is the interaction of basic analytes, such as proteins, with exposed surface silanol groups on the capillary wall. This interaction results in loss of efficiency and irreproducible separations. Several groups have worked on evaluating protein interactions with the silica surface and have reported mathematical models and computer simulations of these interactions (1–3). Typical approaches in addressing the aforementioned problem include working at conditions where the silanol groups are either unionized (4) or fully ionized (5). However, these conditions require working at extremes of pH and which may be unsuitable for many analytes. In addition, silica dissolves at extreme pHs, another limitation of this approach (6). Other approaches in addressing the aforementioned problem include adding compounds (7–9) that compete with the analytes for the sites of interaction on the capillary wall. These additives, however, may adversely affect the separation of analytes.

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Authors
  1. Kannan Srinivasan
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  2. Chris Pohl
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  3. Nebojsa Avdalovic
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Editors and Affiliations

  1. University of Texas Medical Branch, Galveston, TX, USA

    John R. Petersen  & Amin A. Mohammad  & 

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Srinivasan, K., Pohl, C., Avdalovic, N. (2001). Capillary Coatings for Protein Analysis. In: Petersen, J.R., Mohammad, A.A. (eds) Clinical and Forensic Applications of Capillary Electrophoresis. Pathology and Laboratory Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-120-6_3

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  • DOI: https://doi.org/10.1007/978-1-59259-120-6_3

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-113-4

  • Online ISBN: 978-1-59259-120-6

  • eBook Packages: Springer Book Archive

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