Abstract
The synaptic region may be defined as the site of contact between two excitable cells having a specific structural, biochemical, and functional differentiation for the transmission of nerve impulses. As suggested by Du Bois Raymond in 1877, synaptic transmission may be either chemical or electrical, and both mechanisms have been found to occur in the peripheral and central nervous systems. However, chemical synapses are by far the more common and are the only ones that will be considered here from the point of view of their structural and biochemical organization.
The original research contained in this chapter was supported by grants from the Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina, and from the National Institutes of Health, No. NB-06953-02.
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References
J. C. Eccles, The Physiology of Synapses, Springer Verlag, Berlin (1964).
E. De Robertis and H. S. Bennett, Submicroscopic vesicular component in the synapse, Federation Proc. 13: 35 (1954);
E. De Robertis and H. S. Bennett, Some features of the submicroscopic morphology of synapses in frog and earthworm, J. Biophys. Biochem. Cytol. 1: 47–58 (1955).
J. del Castillo and B. Katz, Biophysical aspects of neuromuscular transmission, in Progress in Biophysics (J. A. Butler and B. Katz, Eds.), Vol. 6, pp. 121–170, Pergamon Press, London (1956).
E. De Robertis, Histophysiology of Synapses and Neurosecretion, Pergamon Press, New York (1964).
E. De Robertis, Ultrastructure and cytochemistry of the synaptic region, Science 156: 907–914 (1967).
V. P. Whittaker, The isolation and characterization of acetylcholine-containing particles from brain, Biochem. J. 72: 694–706 (1959).
E. G. Gray and V. P. Whittaker, The isolation of synaptic vesicles from the central nervous system, J. Physiol. (London) 153: 35–37P (1960).
E. De Robertis, A. Pellegrino de Iraldi, G. Rodriguez de Lores Arnaiz, and C. J. Gómez, On the isolation of nerve endings and synaptic vesicles (Meeting of the Sociedad Argentina de Biologia, Mendoza, October, 1960), J. Biophys. Biochem. Cytol. 9: 229–235 (1961).
E. De Robertis, A. Pellegrino de Iraldi, G. Rodriguez de Lores Arnaiz, and L. Salganicoff, Cholinergic and non-cholinergic nerve endings in rat brain. I. Isolation and subcellular distribution of acetylcholine and acetylcholinesterase, J. Neurochem. 9: 23–35 (1962).
K. Kataoka and E. De Robertis, Histamine in isolated small nerve endings and synaptic vesicles of rat brain cortex, J. Pharmacol. Exptl. Therap. 156: 114–125 (1967).
L. Salganicoff and E. De Robertis, Subcellular distribution of glutamic decarboxylase and gamma-aminobutyric alpha-ketoglutaric transaminase, Life Sci. 2: 85–91 (1963);
L. Salganicoff and E. De Robertis, Sub-cellular distribution of the enzymes of the glutamic acid, glutamine and y-aminobutyric acid cycles in rat brain, J. Neurochem. 12: 287–309 (1965).
E. De Robertis, G. Rodriguez de Lores Arnaiz, and A. Pellegrino de Iraldi, Isolation of synaptic vesicles from nerve endings of the rat brain, Nature 194: 794–795 (1962).
E. De Robertis, L. Salganicoff, L. M. Zieher, and G. Rodriguez de Lores Arnaiz, Acetylcholine and cholinacetylase content of synaptic vesicles, Science 140:300–301 (1963); E. De Robertis, G. Rodriguez de Lores Arnaiz, L. Salganicoff, A. Pellegrino de Iraldi, and L. M. Zieher, Isolation of synaptic vesicles and structural organization of the acetylcholine system within brain nerve endings, J. Neurochem. 10: 225–235 (1963).
E. De Robertis, M. Alberici, G. Rodriguez de Lores Arnaiz, and J. M. Azcurra, Isolation of different types of synaptic membranes from the brain cortex, Life Sci. 5: 577–582 (1966).
G. Rodriguez de Lores Arnaiz, M. Alberici, and E. De Robertis, Ultrastructural and enzymatic studies of cholinergic and noncholinergic synaptic membranes isolated from brain cortex, J. Neurochem. 14: 215–225 (1967).
E. G. Lapetina, E. F. Soto, and E. De Robertis, Gangliosides and acetylcholinesterase in isolated membranes of the rat brain cortex, Biochim. Biophys. Acia 135: 33–43 (1967).
V. P. Whittaker, I. A. Michaelson, and R. J. A. Kirkland, The separation of synaptic vesicles from nerve ending particles (“synaptosomes”), Biochem. J. 90: 293–303 (1964).
R. E. McCaman, G. Rodriguez de Lores Arnaiz, and E. De Robertis, Species differences in subcellular distribution of choline acetylase in the CNS. A study of choline acetylase, acetylcholinesterase, 5-hydroxytryptophan decarboxylase, and monoamine oxidase in four species, J. Neurochem. 12: 927–935 (1965).
E. De Robertis, J. M. Azcurra, and S. Fiszer, Ultrastructure and cholinergic binding capacity of junctional complexes isolated from rat brain, Brain Res. 5: 45–56 (1967).
E. De Robertis, Adrenergic endings and vesicles isolated from brain, Pharmacol. Rev. 18: 413–424 (1966).
K. Uchizono, Characteristics of excitatory and inhibitory synapses in the central nervous system of the cat, Nature 207: 642–643 (1965).
E. De Robertis, O. Z. Sellinger, G. Rodriguez de Lores Arnaiz, M. Alberici, and L. M. Zieher, Nerve endings in methionine sulphoximine convulsant rats, a neurochemical and ultrastructural study, J. Neurochem. 14: 81–89 (1967).
C. W. Cotman and H. R. Mahler, Resolution of insoluble proteins in rat brain subcellular fractions, Arch. Biochem. Biophys. 120: 384–396 (1967).
L. Austin and I. G. Morgan, Incorporation of 14C-labelled leucine into synaptosomes from rat cerebral cortex “in vivo, ” J. Neurochem. 14: 377–387 (1967).
K. von Hungen, H. R. Mahler, and W. J. Moore, Turnover of protein and RNA in synaptic subcellular fractions of rat brain, J. Biol. Chem. 243: 1415–1423 (1968).
V. P. Whittaker, Some properties of synaptic membranes isolated from the central nervous system, Ann. N.Y. Acad. Sci. 137: 982–998 (1966).
E. G. Lapetina, E. F. Soto, and E. De Robertis, Lipids and Proteolipids in isolated sub-cellular membranes of rat brain cortex, J. Neurochem. 15: 437–445 (1968).
H. Weigant, The subcellular localization of gangliosides in the brain, J. Neurochem. 14: 671674 (1967).
V. P. Whittaker and M. N. Sheridan, The morphology and acetylcholine content of isolated cerebral cortical synaptic vesicles, J. Neurochem. 12: 363–372 (1965).
L. A. Barker, J. Amaro, and P. S. Guth, Release of acetylcholine from isolated synaptic vesicles. I. Methods for determining the amount released, Biochem. Pharmacol. 16: 2181–2187 (1967).
A. Sattin, The synthesis and storage of acetylcholine in the striatum, J. Neurochem. 13: 515–524 (1966).
R. I. Birks and F. C. MacIntosh, Acetylcholine metabolism of a sympathetic ganglion, Can. J. Biochem. Physiol. 39: 787–827 (1961).
E. De Robertis, Electron microscope and chemical study of binding sites of brain biogenic amines, in Progress in Brain Research (H. E. and W. A. Himwich, Eds.), Vol. 8, pp. 118–136, Elsevier, Amsterdam (1964).
E. De Robertis, A. Pellegrino de Iraldi, G. Rodriguez de Lores Arnaiz, and L. M. Zieher, Synaptic vesicles from rat hypothalamus. Isolation and norepinephrine content, Life Sci. 4: 193–201 (1965).
L. M. Zieher and E. De Robertis, Distribuci6n subcelular de noradrenalina y dopamina en cerebro de rata, VI Congreso de la Asociacibn Latinoamericana de Ciencias Fisiolôgicas, Vina del Mar, Chile, November, 1964, p. 150 (1964).
L. M. Zieher and E. De Robertis, Subcellular localization of 5-hydroxytryptamine in rat brain, Biochem. Pharmacol. 12: 596–598 (1963).
E. W. Maynert, R. Levi, and A. J. De Lorenzo, The presence of norepinephrine and 5-hydroxytryptamine in vesicles from disrupted nerve-ending particles, J. Pharmacol. Exptl. Therap. 144: 385–392 (1964).
E. W. Maynert and K. Kuriyama, Some observations on nerve ending particles and synaptic vesicles, Life Sci. 3: 1067–1087 (1964).
Dj. Palaie and Z. Supek, Liberation of brain 5-hydroxytryptamine and noradrenaline by X-ray treatment in the new born and adult rat, J. Neurochem. 13: 705–709 (1966).
G. Rodriguez de Lores Arnaiz and E. De Robertis, 5-Hydroxytryptophan decarboxylase activity in nerve endings of the rat brain, J. Neurochem. 11: 213–219 (1964).
E. Roberts and K. Kuriyama, Biochemical-physiological correlations in studies of the y-aminobutyrkc acid system, Brain Res. 8: 1–35 (1968).
J. K. Saelens and L. T. Potter, Subcellular localization of choline acetyltransferase in rat brain cortex, Federation Proc. 25: 451 (1966).
S. Tucek, On the subcellular localization and binding of choline acetyltransferase in the cholinergic nerve endings of the brain, J. Neurochem. 13: 1317–1327 (1966).
S. Tuk, Subcellular distribution of acetyl-CoA synthetase, ATPcitrate lyase, citrate synthase, choline acetyltransferase, fumarate hydratase and lactate dehydrogenase in mammalian brain tissue. J. Neurochem. 14: 531–545 (1967).
R. W. Albers, G. Rodriguez de Lores Arnaiz, and E. De Robertis, Sodium-potassiumactivated ATPase and potassium-activated p-nitrophenylphosphatase: a comparison of their subcellular localizations in rat brain, Proc. Natl. Acad. Sci. U.S. 53: 557–564 (1965).
R. J. A. Hosie, The localization of adenosine triphosphatases in morphologically characterized fractions of guinea-pig brain, Biochem. J. 96: 404–412 (1965).
M. Germain and P. Proulx, Adenosintriphosphatase activity in synaptic vesicles of rat brain, Biochem. Pharmacol. 14: 1815–1819 (1965).
K. Kadota, S. Mori, and R. Imaizumi, The properties of ATPase of synaptic vesicles fraction. J. Biochem. (Tokyo) 61: 424–432 (1967).
H. Yoshida, K. Kadota, and H. Fujisawa, Adenosine triphosphate dependent calcium binding of microsomes and nerve endings, Nature 212: 291–292 (1966).
E. G. Lapetina, G. Rodriguez de Lores Arnaiz, and E. De Robertis, 32P incorporation into different membranous structures separated from rat cerebral cortex, J. Neurochem. 16: 101–106 (1969).
L. M. Seminario, N. Hren, and C. J. Gomez, Lipid distribution in subcellular fractions of the rat brain, J. Neurochem. 11: 197–207 (1964).
G. B. Koelle, Evidence for differences in primary fractions of acetylcholinesterase at different synapses and neuroeffector junctions, in Regional Neurochemistry (S. S. Kety and J. Elkes, Eds.), pp. 312–323, Pergamon Press, Oxford (1961).
J. C. Skou, Enzymatic basis for active transport of Na and K+ across cell membrane, Physiol. Rev. 45: 596–617 (1965).
J. Clausen and B. Formby, Effect of noradrenaline on phosphatase activity in synaptic membranes of the rat brain, Nature 213: 389–390 (1967).
O. Z. Sellinger and I. De Balbian Verster, Glutamine synthetase of rat cerebral cortex: intracellular distribution and structural latency, J. Biol. Chem. 237: 2836–2844 (1963).
E. W. Sutherland, I. Dye, and R. W. Butcher, The action of epinephrine and the role of the adenyl cyclase system in hormone action, Recent Progr. Hormone Res. 21: 623–642 (1965).
E. De Robertis, G. Rodriguez de Lores Arnaiz, M. Alberici, R. W. Butcher, and E. W. Sutherland, Subcellular distribution of adenyl cyclase and cyclic phosphodiesterase in rat brain cortex, J. Biol. Chem. 242: 3487–3493 (1967).
S. Kakiuchi and T. W. Rall, Effects of norepinephrine and histamine on levels of adenosine 3’,5’ phosphate (3,5-AMP) in brain slices, Federation Proc. 24: 150 (1965).
J. M. Azcurra and E. De Robertis, Binding of dimethyl-14C-d-tubocurarine, methyl 14C-hexamethonium and 3H alloferine by isolated synaptic membranes of brain cortex, Intern. J. Neuropharmacol. 6: 15–26 (1967).
S. Fiszer and E. De Robertis, Subcellular distribution and nature of the a-adrenergic receptor in the CNS. Lift Sci. 7: 1093–1103 (1968).
E. De Robertis, S. Fiszer, and E. F. Soto, Cholinergic binding capacity of proteolipids from isolated nerve-endings membranes, Science 158: 928–929 (1967).
E. De Robertis, E. G. Lapetina, J. Pecci Saavedra, and E. F. Soto, In vivo and in vitro action of antisera against isolated nerve ending of brain cortex, Life Sci. 5: 1979–1989 (1966).
F. Wald, A. Mazzuchelli, E. Lapetina, and E. De Robertis, The effect of antiserum against nerve ending membranes from cat cerebral cortex on bioelectrical activity of mollusc neurons, Exptl. Neurol. 21: 336–345 (1968).
G. Rodriguez de Lores Arnaiz and E. De Robertis, Cholinergic and non-cholinergic nerve endings in the rat brain. II. Subcellular localization of monoamine oxidase and succinate dehydrogenase, J. Neurochem. 9: 503–508 (1962).
S. H. Barondes, On the site of synthesis of the mitochondria) protein of nerve endings, J. Neurochem. 13: 721–727 (1966).
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De Robertis, E., de Lores Arnaiz, G.R. (1969). Structural Components of the Synaptic Region. In: Lajtha, A. (eds) Handbook of Neurochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-7321-4_15
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