Abstract
This paper presents part of the results obtained during an ICBT short term scientific exchange mission at the University of Pisa, Dipartimento di Chimica e Chimica Industriale. Several samples of 3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) with comonomer contents in the range of 2,5 to 76 % were produced in fed batch fermentations with different strains under varying cultivation conditions. These samples were characterized by DSC, TGA, GPC, 1H-NMR. Under certain PHA producing conditions, PHA quality and processability are rather bad due to an inhomogeneous distribution of the 3HV units in the polymer while polymers from other fermentations have excellent properties. Analysis of these fermentation conditions resulted in very important hints for improvement of existing and development of future fermentation processes. A strong dependence of the molecular weight distribution of the polymer on the main carbon source used in the fermentation was also found.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Dawes, E. A., and Senior, P. J., 1973, The role and regulation of energy reserve polymers in micro-organisms. Adv. Microbiol. Physiol. 10: 135
Anderson, A. J., and Dawes, E. A., 1990, Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Rev. 54: 450
Braunegg, G., Lefebvre, G., and Genser, K., 1998, Polyhydroxyalkanoates, Biopolyesters from renewable resources. J. Biotechnol. 65: 127
Braunegg, G., and Bogensberger, B., 1985, Zur Kinetik des Wachstums und der Speicherung von Poly-D(-)-3-hydroxybuttersäure bei Alcaligenes latus. Acta Biotechnol. 4: 339
Braunegg, G., Lefebvre, G., Renner, G., Zeiser, A., Haage, G., and Loidl-Lanthaler, K., 1995, Kinetics as a tool for polyhydroxyalkanoate production optimisation. Can.J. Microbiol. 41: 239
Fritsche, K., Lenz, R.W., and Fuller, R.C., 1990, Bacterial polyesters containing branched poly(f3-hydroxyalkanoate) units. Int.J.Biol.Macromol. 12: 92
Fritsche, K., Lenz, R.W., and Fuller, R.C., 1990, Production of unsaturated polyesters by Pseudomonas oleovorans. Int.J.Biol.Macromol. 12:85
Bear, M. M., Leboucherdurand, M. A., Langlois, V., Lenz, R. W., Goodwin, S., and Guerin, P., 1997, Bacterial Poly-3-Hydroxyalkanoates with Epoxy Groups in the SideChains. React. Funct. Polymers 34: 65
Kim, Y. B., Lenz, R. W., and Fuller, R. C., 1991, Preparation and characterization of poly(ß-hydroxyalkanoates) obtained from Pseudomonas oleovorans grown with mixtures of 5-phenylvaleric acid and n-alkanoic acids. Macromolecules 24: 5256
Abe, C., Taima, Y., Nakamura, Y., and Doi, Y., 1990, New bacterial copolyesters of 3-hydroxyalkanoates and 3-hydroxy-ω-fluoroalkanoates by Pseudomonas oleovorans. Polym. Commun. 31: 404
Doi, Y., and Abe, C., 1990, Biosynthesis and characterization of a new bacterial copolyester of 3-hydroxyalkanoates and 3-hydroxy-ω-chloroalkanoates. Macromolecules 23: 3705
Kim, Y. B., Lenz, R. W., and Fuller, R. C., 1992, Poly(3-hydroxyalkanoate) copolymers containing brominated repeating units produced by Pseudomonas oleovorans. Macromolecules 25: 1852
Saito, Y., Nakamura, S., Hiramitsu, M., and Doi, Y., 1996, Microbial Synthesis and Properties of Poly(3- Hydroxybutyrate-co-4-Hydroxybutyrate). Polym. Int. 39: 169
Doi, Y., Tamaki, A., Kunioka, M., and Soga, K., 1987, Biosynthesis of terpolyestecs of 3-hydroxybutyrate, 3-hydroxyvalerate, and 5-hydroxyvalerate in Alcaligenes eutrophus from 5-chloropentanoic and pentanoic acids. Makromol. Chem., Rapid Commun. 8: 631
Kunioka, M., Nakamura, Y., and Doi, Y., 1988, New bacterial copolyesters produced in Alcaligenes eutrophus from organic acids. Polym. Commun. 29: 174
Doi, Y., 1990, Microbial polyesters, VCH Publishers Inc., New York
Madden L.A. et al., 1999, Chain termination in polyhydroxyalkanoate synthesis: involvement of exogenous hydroxy-compounds as chain transfer agents. lnt. J. Biol. Macromol. 25: 43
Hori K. et al., 1994, Effects of culture conditions on molecular weights of poly(3-hydroxyalkanoates) produced by Pseudomonas putida from octanoate. Biotechnol. Lett. 16 (7): 709
Madden L.A. and Anderson, A.J., 1998, Synthesis and characterization of poly(3-hydroxy-butyrate and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) polymer mixtures produced in high-density fed-batch cultures of Ralstonia eutropha (Alcaligenes eutrophus). Macromolecules 31: 5660
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer Science+Business Media New York
About this chapter
Cite this chapter
Schellauf, F., Fernandes, E.G., Braunegg, G., Chiellini, E. (2001). Properties of PHAs and their Correlation to Fermentation Conditions. In: Chiellini, E., Gil, H., Braunegg, G., Buchert, J., Gatenholm, P., van der Zee, M. (eds) Biorelated Polymers. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-3374-7_10
Download citation
DOI: https://doi.org/10.1007/978-1-4757-3374-7_10
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4419-3369-0
Online ISBN: 978-1-4757-3374-7
eBook Packages: Springer Book Archive