Abstract
Previous investigations have indicated a positive correlation between the proliferative and metabolic activities of tumor cells in monolayer culture (Freyer et al., 1984; Freyer and Sutherland, 1985; Walenta and Mueller-Klieser, 1987). On the other hand, no difference in the local oxygen consumption has been found between highly proliferating outer cell areas and non-proliferating inner cellular regions in multicellular tumor spheroids (Mueller-Klieser, 1984, 1987). Therefore, the interrelationship among metabolism, proliferation, and cellular morphology was investigated systematically in tumor cells both in monolayer and spheroid culture.
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References
J.P. Freyer, R.M. Sutherland (1985). A reduction in the in situ rates of oxygen and glucose consumption of cells in EMT6/R0 spheroids during growth. J. Cell. Physiol. 124: 516–524.
J.P. Freyer, E. Tustanoff, A.J. Franko, R.M. Sutherland (1984). In situ oxygen consumption rates of cells in V-79 multicellular spheroids during growth. J. Cell. Physiol. 11818: 53–61.
D.P. Jones (1984). Effect of mitochondrial clustering on O2 supply in hepatocytes, Am. J. Physiol. 247: C83–C89.
W. Mueller-Klieser (1984). Method for determination of oxygen consumption rates and diffusion coefficients in multicellular spheroids, Biophys. J. 46: 343–348.
W. Mueller-Klieser (1987). Multicellular spheroids. A review on cellular aggregates in cancer research, J. Cancer Res. Clin. Oncol. 113: 101–122.
W. Mueller-Klieser, R. Zander, P. Vaupel (1986). A new photometric method for oxygen consumption measurements in cell suspensions, J. Applied Physiol. 61: 449–455.
R.M. Sutherland (1988). Cell and environment interactions in tumor micro-regions: The multicell spheroid model, Science 240: 177–184.
S. Walenta, W. Mueller-Klieser (1987). Oxygen consumption rate of tumor cells as a function of their proliferative status, Adv. Exp. Med. Biol. 215: 389–391.
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© 1989 Plenum Press, New York
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Walenta, S., Bredel, A., Karbach, U., Kunz, L., Vollratt, L., Mueller-Klieser, W. (1989). Interrelationship Among Morphology, Metabolism, and Proliferation of Tumor Cells in Monolayer and Spheroid Culture. In: Rakusan, K., Biro, G.P., Goldstick, T.K., Turek, Z. (eds) Oxygen Transport to Tissue XI. Advances in Experimental Medicine and Biology, vol 248. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5643-1_96
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DOI: https://doi.org/10.1007/978-1-4684-5643-1_96
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