Abstract
Alkaline phosphatase (EC 3.1.3.1), a zinc metallo-enzyme, is irreversibly inhibited by ascorbate, probably through a modification of active site environment (Martorana et al., 1986; Miggiano et al., 1984). A metal substituted form was prepared from bovine kidney alkaline phosphatase purchased from Calbiochem (La Jolla, CA, U.S.A.) and further purified as described (Martorana et al., 1986). Cobalt can replace zinc yielding a partially active phosphohydrolase, which lacks phosphotransferase activity (Ensinger et al., 1978) and presents a relevant visible absorption (Simpson and Vallee, 1968).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Chappelet-Tordo, D., Iwatsubo, M., and Lazdunski, M., 1974, Biochemistry, 13:3754.
Chen, R. F., 1971, Arch. Biochem. Biophys., 142:552.
Csopak, H., Falk, K. E., and Szajn, H., 1972, Biochim. Biophys. Acta, 258:466.
Donovan, J. W., 1969, in: “Physical Principles and Techniques of Protein Chemistry”, S. J. Leach, ed., Academic Press, New York.
Ensinger, H. A., Pauly, H. E., Pfleiderer, G., and Stiefel, T., 1978, Biochim. Biophys. Acta, 527:532.
Martorana, G. E., Meucci, E., Ursitti, A., Miggiano, G.A.D., Mordente, A., and Castelli, A., 1986, Biochem. J., 240, in press.
Miggiano, G.A.D., Mordente, A., Martorana, G.E., Meucci, E., and Castelli, A., 1984, Biochim. Biophys. Acta, 789:343.
Myer, Y. P., Thallum, K. K., and Pande, A., 1980, J. Biol. Chem., 255:9666.
Simpson, R. T., and Vallee, B. L., 1968, Biochemistry, 7:4343.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Plenum Press, New York
About this paper
Cite this paper
Martorana, G.E., Meucci, E., Miggiano, G.A.D., Mordente, A., Santini, S.A., Castelli, A. (1989). Metal Substitution and Fluorescence Modifications in Ascorbate-Alkaline Phosphatase Interaction. In: Jameson, D.M., Reinhart, G.D. (eds) Fluorescent Biomolecules. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5619-6_38
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5619-6_38
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5621-9
Online ISBN: 978-1-4684-5619-6
eBook Packages: Springer Book Archive