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Estimation of Concentration Ratios and the Redox States of the Cytochromes from Noisy Reflection Spectra Using Multicomponent Analysis Methods

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Oxygen Transport to Tissue VIII

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 200))

Abstract

To quantify tissue reflection spectra, multicomponent analysis methods have been successfully used. These methods reconstruct the measured reflection spectra using a set of basic absorption spectra, which describe the single chromophoric substances of the multicomponent system, and applying the Kubelka-Munk algorithm (Kubelka and Munk, 1931; Hoffmann et al., 1984). Since basic absorption spectra differ in distinctness and sharpness of absorption peaks, multicomponent analysis methods give concentration ratios of the components with different uncertainties.

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References

  • Dahlquist, G., and Anderson, N., 1974, Numerical Methods, Englewood Cliffs, New York.

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  • Kubelka, K., and Munk, F., 1931, Ein Beitrag zur Optik der Farbanstriche, Z. Techn. Phys., 11a: 593–603.

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  • Hoffmann, J., Heinrich, U., Ahmad, H.R., and Lü;bbers, D.W., 1984, Analysis of tissue reflection spectra obtained from brain or heart, using two-flux theory for non-constant light scattering, in: Oxygen Transport to Tissue VI, D. Bruley, H.I. Bicher, D. Reneau (eds.), Plenum Press, New York and London, 1984, pp. 555–563.

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  • Hoffmann, J. and Lü;bbers, D.W., Improved quantitative analysis of reflection spectra obtained from the surface of the isolated perfused guinea pig heart. (this volume)

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  • Lawson, Ch., L., and Hanson, R.J., 1974, Solving least squares problems, Englewood Cliffs, New York.

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© 1986 Plenum Press, New York

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Hoffmann, J., Lübbers, D.W. (1986). Estimation of Concentration Ratios and the Redox States of the Cytochromes from Noisy Reflection Spectra Using Multicomponent Analysis Methods. In: Longmuir, I.S. (eds) Oxygen Transport to Tissue VIII. Advances in Experimental Medicine and Biology, vol 200. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5188-7_16

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  • DOI: https://doi.org/10.1007/978-1-4684-5188-7_16

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5190-0

  • Online ISBN: 978-1-4684-5188-7

  • eBook Packages: Springer Book Archive

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