Abstract
Quantification by NMR of hypoxic stress is best afforded by the ratio of phosphocreatine (PCr) to inorganic phosphate (Pi) (1). The thin cranium and sternum of neonates affords ideal conditions for NMR evaluation of impaired oxidative metabolism (2,3). Brains of animal models (dog and cat) are best studied with muscles retracted with the rf coil placed on the bare skull.31 P and 1H (lactate) signals are time shared with a doubly tuned coil (4). Servo-stabilized hypoxia gives simplified steady state analysis. Hypoxic insults of the brain are evaluated by the integral of the deviation of the PCr/Pi ratio from the normoxic values. Generally, a deviation of 1 unit of PCr/Pi for an interval of 3 hr will lead to a severe metabolic damage and recovery from hypoxia is often 30-fold delayed. Comprehensive monitoring of energy metabolism (PCr/Pi), respiratory chain redox states, intracellular pH and lactate accumulation allow the calculation of integrated pH and lactate “insults” and thus offer new insights on the effects of hypoxia on brain oxidative metabolism.
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Chance, B., Eleff, S., Sokolow, D. and Sapega, A. (1981) Proc. Natl. Acad. Sci. USA 78: 6714–6718.
Chance, B., Younkin, D., Eleff, S., Warnell, R. and Delivoria-Papdopoulos, M. (1983) Ped.Res. 17: 397.
Younkin, D.P., Delevoria-Papadopoulos, M., Kelley, R., Clancy, R., Leigh, Jr. J.S. and Chance, B., (1984) Ped.Res. 18: 385.
Schnall, M.D., Harihara Subramanian,H., Leigh, Jr.J.S., Gyulai, L., McLaughlin, A. and Chance, B. (1985) Soc.Mag.Res.in Med. 63: 401–406.
Subramanian, H.V., Chance, B., Leigh, Jr. J.S., Clark, B.J., Osbakken, M., Egan, J., Nioka, S., Hilberman, M., Donlon, E. and Richardson, M. (1985) Fed. Proc. 44: 676.
Hilberman, M., Harihara Subramanian, V., Haselgrove, J., Egan, J., Gyulai, L. and Chance, B. (1984) J. Cereb. Blood Flow and Metab. 4: 334–342.
Chance, B. and Schoener, B. (1966) J. Biol. Chem. 241: 4577–4587.
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© 1986 Plenum Press, New York
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Chance, B. et al. (1986). P NMR Evaluation of Hypoxic Stress in Brain of Animal Models. In: Longmuir, I.S. (eds) Oxygen Transport to Tissue VIII. Advances in Experimental Medicine and Biology, vol 200. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5188-7_14
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DOI: https://doi.org/10.1007/978-1-4684-5188-7_14
Publisher Name: Springer, Boston, MA
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