Abstract
Central nervous system pharmacology is in the midst of a new stage of development. With the advent of sensitive and specific radioreceptor binding assays for studying drug-receptor interactions, rapid advances have been made in the biochemical investigation of drug and transmitter mechanisms. Our knowledge of the physiological actions of neuroactive drugs has also progressed in recent years, due in large part to the extensive use of the iontophoretic technique coupled with extra- and intracellular recording from cells in the intact brain. Although such in vivo studies provide an important and necessary foundation for any serious investigation of the effects of a drug on the nervous system, detailed information about the site and mode of action of drugs is very difficult to obtain. In vivo electrophysiological studies of neurons in the mammalian brain generally suffer from two difficulties that prohibit detailed analysis: (1) insufficient mechanical stability to permit long-lasting intracellular recordings on a routine basis and (2) an inability to know the equilibrium concentration of drugs in the interstitial space. These drawbacks can be overcome with the use of brain slices, which forms the basis of a strong rationale for the utility of these preparations in neuropharmacological investigations.
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References
Alger, B. E. and Nicoll, R. A., 1979, GABA-mediated biphasic inhibitory responses in hippocampus, Nature, (London) 281:315–317.
Alger, B. E. and Nicoll, R. A., 1982, Feed-forward dendritic inhibition in rat hippocampal pyramidal cells studied in vitro, J. Physiol. (London) 328:105–123.
Andersen, P., 1975, Organization of hippocampal neurons and their connections, in: The Hippocampus, Volume 1 (R. L. Isaacson and K. H. Pribram, eds.), Plenum Press, New York, pp. 155–175.
Andersen, P., Bie, B., Ganes, T., and Mosfeldt Laursen, A., 1978a, Two mechanisms for effects of GABA on hippocampal pyramidal cells, in: Iontophoresis and Transmitter Mechanisms in the Mammalian Central Nervous System (R. Ryall and J. S. Kelly, eds.), Elsevier N. Holland Biomedical Press, pp. 178–181.
Andersen, P., Silfvenius, H., Sundberg, S. H., Sveen, O., and Wigström, H., 1978b, Functional characteristics of unmyelinated fibres in the hippocampal cortex, Brain Res. 144:11–18.
Andersen, P., Eccles, J. C., and Løyning, Y., 1964, Pathway of postsynaptic inhibition in the hippocampus, J. Neurophysiol. 27:608–619.
Andersen, P., Dingledine, R., Gjerstad, L., Langmoen, I. A., and Mosfeldt-Laursen, A., 1980, Two different responses of hippocampal pyramidal cells to application of gamma-amino butyric acid, J. Physiol. (London) 305:279–296.
Azmitia, E. C. and Segal, M., 1979, An autoradiographic analysis of the differential ascending projections of the dorsal and median raphe nuclei in the rat, J. Comp. Neurol. 179:641–668.
Benardo, L. S. and Prince, D. A., 1982a, Cholinergic excitation of mammalian hippocampal pyramidal cells, Brain Res. 249:315–331.
Benardo, L. S. and Prince, D. A., 1982b, Ionic mechanisms of cholinergic excitation in mammalian hippocampal pyramidal cells, Brain Res. 249:333–344.
Ben-Ari, Y., Krnjevic, K., Reiffenstein, R., and Ropert, N., 1981, Intracellular observations on disinhibitory action of acetylcholine in hippocampus, Neuroscience 6:2475–2484.
Biscoe, T. J. and Straughan, D. W., 1966, Micro-electrophoretic studies of neurones in the cat hippocampus, J. Physiol. (London) 183:341–359.
Bostock, E., Dingledine, R., Xu, G., and Chang, K.-J., Epileptiform effect of a mu-opioid receptor agonist, morphiceptin, in the hippocampal slice, submitted.
Chang, K.-J., Cooper, B. R., Hazum, E., and Cuatrecasas, P., 1979, Multiple opiate receptors: Different regional distribution in the brain and differential binding of opiates and opioid peptides, Mol. Pharmacol. 16:91–104.
Collingridge, G. L., Kehl, S. J., and McLennan, H., 1982a, Antagonism of amino acid induced excitation of CA1 hippocampal neurones in vitro, J. Physiol. (London) 322:52P.
Collingridge, G. L., Kehl, S. J., and McLennan, H., 1982b, The effect of excitatory amino acid agonists and antagonists on the Schaffer collateral input to rat CA1 hippocampal neurones, J. Physiol. (London) 322:53P.
Corrigall, W. A. and Linseman, M. A., 1980, A specific effect of morphine on evoked activity in the rat hippocampal slice, Brain Res. 192:227–238.
Crutcher, K. A., Madison, R., and Davis, J. N., 1981, A study of the rat septohippocampal pathway using anterograde transport of horseradish peroxidase, Neuroscience 6:1961–1973.
Dingledine, R., 1981, Possible mechanisms of enkephalin action on hippocampal CA1 pyramidal neurons, J. Neurosci. 1:1022–1035.
Dingledine, R., 1982, Amino acid activated calcium conductance in hippocampal pyramidal cells, Soc. Neurosci. Abstr. 8:796.
Dingledine, R., 1983a, N-Methyl-aspartate activates voltage dependent calcium conductance in rat hippocampal pyramidal neurones, J. Physiol. (London) in press.
Dingledine, R., 1983b, Excitatory amino acids: Modes of action on hippocampal pyramidal cells, Fed. Proc., 42:2281–2285.
Dingledine, R. and Gjerstad, L., 1979, Penicillin blocks hippocampal IPSPs, unmasking prolonged EPSPs, Brain Res. 168:205–209.
Dingledine, R. and Gjerstad, L., 1980, Reduced inhibition during epileptiform activity in the in vitro hippocampal slice, J. Physiol. (London) 305:297–313.
Dingledine, R. and Langmoen, I. A., 1980, Conductance changes and inhibitory actions of hippocampal recurrent IPSPs, Brain Res. 185:277–287.
Dodd, J. and Kelly, J. S., 1979, Is somatostatin an excitatory transmitter in the hippocampus? Nature (London) 273:674–675.
Dodd, J. and Kelly, J. S., 1981, The actions of cholecystokinin and related peptides on pyramidal neurones of the mammalian hippocampus, Brain Res. 205:337–350.
Dodd, J., Kelly, J. S., and Said, S. I., 1979, Excitation of CA1 neurones of the rat hippocampus by the octacosapeptide, vasoactive intestinal polypeptide (VIP), Br. J. Pharmacol. 66:125P.
Dodd, J., Dingledine, R., and Kelly, J. S., 1981, The excitatory action of acetylcholine on hippocampal neurones of the guinea pig and rat maintained in vitro, Brain Res. 207:109–127.
Dunwiddie, T., Mueller, A., Palmer, M., Steward, J., and Hoffer, B., 1980, Electrophysiological interactions of enkephalins with neuronal circuitry in the rat hippocampus. I. Effects on pyramidal cell activity, Brain Res. 184:311–330.
Eccles, J. C., Nicoll, R. A., Oshima, T., and Rubia, F. J., 1977, The anionic permeability of the postsynaptic membrane of hippocampal pyramidal cells, Proc. R. Soc. Lond. [Biol] 198:345–361.
Gähwiler, B. H. and Maurer, R., 1981, Involvement of µ-receptors in the opioid-induced generation of bursting discharges in hippocampal pyramidal cells, Regulatory Peptides 2:91–96.
Gall, C., Brecha, N., Karten, H. J., and Chang, K.-J., 1981, Localization of enkephalin-like immunoreactivity to identified axonal and neuronal populations of the rat hippocampus, J. Comp. Neurol. 198:335–350.
Gold, M. R. and Martin, A. R., 1982, Intracellular Cl- accumulation reduces Cl- conductance in inhibitory synaptic channels, Nature 299:828–830.
Greenwood, R. S. and Winstead, K. K., 1981, Immunocytochemical double labeling for cholecystokinin and somatostatin in the rat hippocampus, Soc. Neurosci. Abstr. 7:100.
Greenwood, R. S., Godar, S. E., Reaves, T. A., and Hayward, J. N., 1981, Cholecystokinin in hippocampal pathways, J. Comp. Neurol. 203:335–350.
Haas, H. L., 1982, Cholinergic disinhibition in hippocampal slices of rat, Brain Res. 233:200–204.
Haas, H. L. and Gähwiler, B. H., 1980, Do enkephalins directly affect calcium-spikes in hippocampal pyramidal cells? Neurosci. Lett., 19:89–92.
Haas, H. L. and Ryall, R. W., 1980, Is excitation by enkephalins of hippocampal neurons in the rat due to presynaptic facilitation or to disinhibition? J. Physiol. (London) 308:315–330.
Haas, H. L., Felix, D., Celio, M. R., and Inagami, T., 1980, Angiotensin II in the hippocampus. A histochemical and electrophysiological study, Experientia 36:1394–1395.
Haas, H. L., Felix, D., and Davis, M. D., 1982, Angiotensin excites hippocampal pyramidal cells by two mechanisms, Cell. Mol. Neurobiol. 2:21–32.
Hablitz, J. J. and Langmoen, I. A., 1982, Excitation of hippocampal pyramidal cells by glutamate in the guinea-pig and rat, J. Physiol. (London) 325:317–331.
Halliwell, J. V. and Adams, P. R., 1982, Voltage clamp analysis of muscarinic excitation in hippocampal neurons, Brain Res. 250:71–92.
Handelmann G., Meyer, D. K., Beinfeld, M. C., and Oertel, W. H., 1981, CCK-containing terminals in the hippocampus are derived from intrinsic neurons: An immunohisto-chemical and radioimmunological study, Brain Res. 224:180–184.
Henricksen S. J., Bloom, F. E., McCoy, F., Ling, N., and Guillemin, R., 1978, ß-endorphin induces non-convulsive limbic seizures, Proc. Nat’l. Acad. Sci. USA 75:5221–5225.
Hounsgaard, J., 1978, Presynaptic inhibitory action of acetylcholine in area CA1 of the hippocampus, Exp. Neurol. 62:787–797.
Kimura, H., McGeer, P. L., Peng, J. H., and McGeer, E. G., 1981, The central cholinergic system studied by choline acetyltransferase immunohistochemistry in the cat, J. Comp. Neurol. 200:151–200.
Knowles, W. D. and Schwartzkroin, P. A., 1981, Local circuit synaptic interactions in hippocampal brain slices, J. Neurosci. 1:318–322.
Koerner, J. F. and Cotman, C. W., 1981, Micromolar L-2-amino-4-phosphonobutyric acid selectively inhibits perforant path synapses from lateral entorhinal cortex, Brain Res. 216:192–198.
Koerner, J. F. and Cotman, C. W., 1982, Response of Schaffer collateral-CA1 pyramidal cell synapses of the hippocampus to analogues of acidic amino acids, Brain Res. 251:105–115.
Krnjevic, K., Pumain, R., and Renaud, L., 1971,The mechanism of excitation by acetylcholine in the cerebral cortex, J. Physiol. (London) 215:247–268.
Lanthorn, T. H. and Cotman, C. W., 1981, Baclofen selectively inhibits excitatory synaptic transmission in the hippocampus, Brain Res. 225:171–178.
Lee, H. K., Dunwiddie, T., and Hoffer, B., 1980, Electrophysiological interactions of enkephalins with neuronal circuitry in the rat hippocampus. II. Effects on interneuron excitability, Brain Res. 184:331–342.
Lindvall, O. and Bjorkland, A., 1974, The organization of the ascending catecholamine neurone systems in the rat brain, as revealed by the glyoxylic acid fluorescence method, Acta Physiol. Scand. 73(suppl 412): l-48.
Loren, I., Emson, P. C., Fahrenkrug, J., Bjorklund, A., Alumets, J., Hakänson, R., and Sundler, F., 1979, Distribution of vasoactive intestinal polypeptide in the rat and mouse brain, Neuroscience 4:1953–1976.
Lorente de Nó, R., 1934, Studies on the structure of the cerebral cortex. II. Continuation of the study of the amnionic system, J. Psychol. Neurol. (Leipzig) 46:113–177.
Lynch, G., Rose, G., and Gall, C., 1978, Anatomical and functional aspects of the septo-hippocampal projections, in: Functions of the Septo-Hippocampal System, Ciba Foundation Symposium no. 58, Elsevier-North Holland, Amsterdam, pp 5–20.
Lynch, G. S., Jensen, R. A., McGaugh, J. L., Davila, K., and Oliver, M. W., 1981, Effects of enkephalin, morphine and naloxone on the electrical activity of the in vitro hip-pocampal slice preparation, Exp. Neurol. 71:527–540.
MacDonald, J. F. and Wojtowicz, J. M., 1982, The effects of L-glutamate and its analogues upon the membrane conductance of central murine neurones in culture, Canad. J. Physiol. Pharmacol. 60:282–296.
Masukawa, L. M. and Prince, D. A., 1982, Enkephalin inhibition of inhibitory input to CA1 and CA3 pyramidal neurons in the hippocampus, Brain Res. 249:271–280.
McGinty, J. F., Henricksen, S. J., Goldstein, A., Terenius, L., and Bloom, F. E., 1983, Dynorphin is contained within hippocampal mossy fibers: Immunochemical alterations after kainic acid administration and colchicine-induced neurotoxicity, Proc. Natl. Acad. Sci. USA 80:589–593.
Moore, R. Y. and Halaris, A. E., 1975, Hippocampal innervation by serotonin neurons of the midbrain raphe in the rat, J. Comp. Neurol. 152:163–174.
Nadler, J. V., Vaca, K. W., White, W. F., Lynch, G. S., and Cotman, C. W., 1976, Aspartate and glutamate as possible transmitters of excitatory hippocampal afférents, Nature (London) 260:538–540.
Nadler, J. V., White, W. F., Vaca, K. W., Perry, B. W., and Cotman, C. W., 1978, Biochemical correlates of transmission mediated by glutamate and aspartate, J. Neurochem. 31:147–155.
Nicoll, R. A., Alger, B. E., and Jahr, C. E., 1980, Enkephalin blocks inhibitory pathways in the vertebrate CNS, Nature (London) 287:22–25.
Ribak, C. E., Vaughn, J. E., and Saito, K., 1978, Immunocytochemical localization of glutamic acid decarboxylase in neuronal sonata following colchicine inhibition of axonal transport, Brain Res. 140:315–332.
Robinson, J. H. and Deadwyler, S. A., 1981, Intracellular correlates of morphine excitation in the hippocampal slice preparation, Brain Res. 224:375–387.
Schwartzkroin, P. A. and Mathers, L. H., 1978, Physiological and morphological identification of a non-pyramidal hippocampal cell type, Brain Res. 157:1–10.
Segal, M., 1982, Multiple actions of acetylcholine at a muscarinic receptor studied in the rat hippocampal slice, Brain Res. 246:77–87.
Silfvenis, H., Olofsson, S., and Ridderheim, P.-A., 1980, Induced epileptiform activity evoked from dendrites of hippocampal neurones, Acta Physiol. Scand. 108:109–111.
Storm-Mathisen, J., 1977, Localization of transmitter candidates in the brain: The hippo- campal formation as a model, Prog. Neurobiol. 8:119–181.
Thalmann, R. H., Peck, E. J., and Ayala, G. F., 1981, Biphasic response of hippocampal pyramidal neurons to GABA, Neurosci. Lett. 21:319–324.
Urea, G., Frenk, H., Liebeskind, J. C., and Taylor, A. N., 1977, Morphine and enkephalin: Analgesic and epileptic properties, Science 197:83–86.
Valentino, R. J. and Dingledine, R., 1981, Presynaptic inhibitory effect of acetylcholine in the hippocampus, J. Neurosci. 1:787–792.
Valentino, R. J. and Dingledine, R., 1982, Pharmacological characterization of opioid effects in the rat hippocampal slice, J. Pharmacol. Exp. Ther. 223:502–509.
Vincent, S. R., Kimura, H., and McGeer, E. G., 1981, Organization of substance P fibers within the hippocampal formation demonstrated with a biotin-avidin immunoperoxidase technique, J. Comp. Neurol. 199:113–123.
Watkins, J. C. and Evans, R. H., 1981, Excitatory amino acid transmitters, Annu. Rev. Pharmacol. Toxicol. 21:165–204.
White, W. F., Nadler, J. V., Hamberger, A., Cotman, C. W., and Cummins, J. T., 1977, Glutamate as a transmitter of hippocampal perforant path, Nature (London) 270:356–357.
White, W. F., Nadler, J. V., and Cotman, C. W., 1979, The effect of acidic amino acid antagonists on synaptic transmission in the hippocampal formation in vitro, Brain Res. 164:177–194.
Wieraszko, A. and Lynch, G., 1979, Stimulation-dependent release of possible transmitter substances from hippocampal slices studied with localized perfusion, Brain Res. 160:372–376.
Wong, R. K. S. and Prince, D. A., 1979, Dendritic mechanisms underlying penicillin-induced epileptiform activity, Science 204:1228–1231.
Wong, R. K. S. and Watkins, D. J., 1982, Cellular factors influencing GABA response in hippocampal pyramidal cells, J. Neurophysiol. 48:938–951.
Yamamoto, C. and Kawai, N., 1967, Presynaptic action of acetylcholine in thin sections from the guinea pig dentate gyrus in vitro, Exp. Neurol. 19:176–187.
Zieglgänsberger, W., French, E., Siggins, G., and Bloom, F., 1979, Opioid peptides may excite hippocampal pyramidal neurons by inhibiting adjacent inhibitory interneurons, Science 205:415–417.
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Dingledine, R. (1984). Hippocampus. In: Dingledine, R. (eds) Brain Slices. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4583-1_5
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