Abstract
The expression of DNP- and TNP-specific B cells in spleens of neonatal BALB/c mice was analyzed by the in vitro splenic focus technique. B cells of these specificities were found to be present in slightly higher frequency in neonatal than in adult spleens. The parameters of stimulation of neonatal B cells were similar to those of adult B cells but the antibody-forming cell progeny of neonatal B cells produce predominantly γM rather than γG antibody and produce less antibody than the progeny of adult B cells. Isoelectric focusing analyses of monoclonal antibodies derived from neonatal B cells stimulated in vitro with DNP or TNP revealed that over 90% of the antibodies could be identified as belonging to one of six predominant clonotypes, three specific for DNP and three for TNP. While individual neonates rarely expressed all of the predominant clonotypes, B cells of each of the six clonotypes were found in several donors. When B cells of a given predominant clonotype were present in an individual many such B cells could be found and in many cases the entire DNP- or TNP-specific B cell population of an individual could be accounted for by B cells of a single clonotype. These findings are discussed in terms of the diversity of clonotype specificities available in neonates, the kinetics of development of cells within a clonotype, and factors that may play a role in controlling the expression of B cell clones.—Klinman, N. R. and J. L. Press. Expression of specific clones during B cell development. Federation Proc. 34: 47–50, 1975.
From Session II, Gene Regulation in Differentiation and Development, of the FASEB Conference on Biology of Development and Aging, presented at the 58th Annual Meeting of the Federation of American Societies for Experimental Biology, Atlantic City, N.J., April 9, 1974.
Supported by Research Grant Al-08778 from the Public Health Service.
Recipient of a Public Health Service Career Development Award (1-K04-A1-33983) from the National Institute of Allergy and Infectious Diseases.
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Abbreviations
- B cell:
-
antibody forming cell precursor
- DNP:
-
2, 4-dinitrophenyl
- Fl:
-
fluorescein
- Hy:
-
Limulus polyphemus hemocyanin
- TNP:
-
2, 4, 6-trinitrophenyl
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Klinman, N.R., Press, J.L. (1975). Expression of specific clones during B cell development. In: Thorbecke, G.J. (eds) Biology of Aging and Development. Faseb Monographs, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2631-1_10
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